CAbS offers personalized development of immunoassays. Antibodies and other necessary immunoreagents can be synthesized.
The development of the immunoassay includes different stages:
The purification of the antibodies includes the isolation of the antibody from the serum (polyclonal antibodies) or culture supernatant from hybridoma cell lines (monoclonal antibodies). The services offers previous advise on the different possibilities for purification according to the needs of the user.
The methods of purification that we offer vary from crude methods (precipitation of the proteins from the sample including any antibody present) to general purification (purification by affinity of certain classes of antibodies without taking account of the specificity to the antigen) to specific (purification by affinity of the antibodies which join specifically to a definite antigen).
Hybridoma cell culture in ultralow serum medium. We use CELLine™ Bioreactor Technologys to produce monoclonal antibodies.
The mAb development contains the following phases:
The service has a standard immunization protocol that includes monthly inoculations with Freund adjuvant during a period of 4 months. This protocol can be adapted to final project objective.
To determine the progress of the immune response, a bleed is taken after 10 days after the second immunization to obtain antiserum and the titer is determined through ELISA.
Among the controls is included obtaining preimmune serum from the animals obtained from the blood previously to the first injection. At the end of the process 50-70 mL of hyperimmune serum is obtained per animal from final production bleed.
Protein and hapten conjugation services with different types of molecules such as: Enzymes, Fluorophores, Biotin, KLH, BSA, gold particles, magnetic particles, nanotubes…
Preparation of antibody fragments.
Modification of the peptides during and post synthesis to meet user requirements: