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Equipments

Equipments

U7-E02. ToF-SIMS (Time of the Flight Secondary Ion Mass Spectroscopy)

• ToF-SIMS (Time of the Flight Secondary Ion Mass Spectroscopy)

ION-TOF IV (IONTOF, Münster, Germany) equipped with the state-of-the –art Bi cluster primary ion gun for analysis, and O2 and Cs ion guns for sputtering in the depth profiling mode.
Charge compensation capability using a pulsed electron-gun and/or O2 flood gas allows surface analysis and depth profiling of highly insulating samples
Large sample stage capable of holding wafers up to 3″ in diameter/width and 0.5″ in thickness. Samples sizes from few mm up to 10cm. Motorized sample stage that allows for automated analysis of large areas. Heating/cooling stage.

• High mass resolution, to distinguish species of similar nominal mass (mass resolution is at least 0.00x amu); Particles with the same nominal mass (e.g. Si2 and Fe, both with amu = 56 ) can be clearly distinguish.
• Mass range of 0-10,000 amu; ions (positive or negative), isotopes, and molecular compounds (including polymers, organic compounds, and up to amino acids fragments) can be detected.
• High sensitivity for trace elements or compounds, on the order of ppm to ppb for most species;
• Elemental and chemical mapping on a sub-micron scale;
• Depth profiling capabilities; sequential sputtering of surfaces allow analysis of elemental composition on materials from 1nm up to 10µm in depth (typical sputtering rates are ~10nm/minute).
• Retrospective analysis. Every pixel of a ToF-SIMS map represents a full mass spectrum. This allows an analyst to retrospectively produce maps for any mass of interest, and to interrogate regions of interest (ROI) for their chemical composition via computer processing after the dataset has been instrumentally acquired.
• Surveys of all masses on material surfaces; these may include single ions (positive or negative), individual isotopes, and molecular compounds;
• Surface analysis of insulating and conducting samples;

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U7-E01. Ebeam/SEM (Nova NanoSEM 230/Raith)

• Nova NanoSEM 230

›› High and Low Vacuum field emission Scanning
Electron Microscope (SEM ) incorporating:

•• Resolution: 1.6 nm at 1 kV (ETD and TLD detectors) in High Vacuum.
•• Resolution: 1.8 nm at 3 kV (LVD detectors) in Low Vacuum.
•• Field emission filament -> High-resolution images even of non-conductive samples.
•• Backscattered electron detector (vCD detector):
High-resolution images of backscattered electrons.
•• 6-channel preamplifier for detectors of solid state.
•• Compressor.
•• Cryo Can, anti-contamination system.
•• Thermoflex closed wáter circuit.
›› Electron beam lithography system with:
•• High writing resolution (maximum speed of 6 MHz and control of the dwell time < 2 ns). •• Electrostatic Beam Blanker. •• Picoammeter exchanger box. •• Keithley picoammeter. •• Advanced nanolithography system for microscope. •• Beam Blanker amplification electronics •• Software for correcting the Proximity Effect. •• Universal sample carrier for Lithography. •• EBL Starter kit.

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U14-E01. Cell culture rooms

Cell Culture Room 1:
Vertical Laminar Flow Cabinet Herasafe® (Formimetal).
CO2 incubator Heracell 150 (Thermo)
Nomarski phase inverted microscope and fluorescencia con cámara (Nikon)
Camera Color 1CCD (Nikon)
Cooled Camera black and white (Nikon)
Vacuum Pump ME4 (Vacuubrand)
Cell culture water Baths (Controltecnica)

Cell Culture Room 2:
Vertical Laminar Flow Cabinet Herasafe® (Formimetal)
CO2 incubator Heracell 150 (Thermo)
CO2 incubator Heracell 150 with CO2 control (Thermo)
phases inverted microscope with fluorescence (NIKON)
Vacuum Pump ME4 (Vacuubrand)
Cell Counter (Invitrogen)

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U14-E02. Molecular biology

Molecular Biology
Fume Hood (Formimetal)
Stereo microscope with camera and digital image set (NIKON)
light source (for the glass) (NIKON)
Refrigerated centrifuge for microtubs Biofuge® Pico (HERAEUS)
Digital circulation thermostatic bath (GRANT)
Thermostatic bath (for cell cultures temperar medios) (MEDIGEN))
Pipette controller pipette Powerpette-plus (IZASA)
Pipettes de volumen variable continuo with accessories (RAININ)
Vertical Freezer -20ºC (FIOCHETTI)
Freezer +2/+8ºC (CLASS ELECTRONIC)
Laboratory vertical freezer Herfreeze ®HFU-486-Basic (-80ºC) (HERAEUS)
Crioconservtion system with accessories
Liquid Nitrogen tank (taylor wharton)
Thermocycler (THERMO)
Sterilization incubator (Modelo UT ) (HERAEUS)
G-Box Translumitator and accessories (PC, Sofware…) (SYNGENE)
Electrophoresis system (IZASA)
Electrophoresis vertical jar
Electrophoresis horizontal (big) jar
Electrophoresis horizontal (small) jar
Rotary shaker with incubation (HEIDOLPH)
Magnetic stirrer with heating (ARE)
Analytical balance 210gr, 0,01 gr. (ACCULAB)
Precision analytical balance (OHAUS)
Ultrasonic water bath (SELECTA)
Crushed ice machine (Schottman)
Microwave (LG)
Pipette controller (POWERPETTE PLUS)
Thermoblock (Labolan)
Vortex 230 V, EU (VWR)
Freezer +4ºC/-20ºC
UV mini-1240 Spectrophotometer (SHIMADZU)
ELISA plate reader, Synergy MX (BIOTEK)
Flow cytometry FACSCalibur (BD)
pH-Meter (Symphony)
i-Blot (Invitrogen)
Autoclave

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U13-E02. Machines for mechanical tests

Machines for mechanical tests:

• Instron MicroTester 5848 and 5548: electromechanical machines for displacement or load control tests:

•• load range from 1mN to 2kN at full scale.
•• load cells of:+/- 5, 50, 500 and 1000N.
•• synchronous data capture in all the channels up to
500 Hz.
• Biaxial Instron MicroTester 8874: axial-torsional, servohydraulic testing machine:
•• load capacity of 25 kN and 100 Nm.
•• fatigue tests at a low number of cycles.

›› Software for data control and analysis.
›› Accessories and tools especially designed by the group, which allow a wide flexibility in designing tests, allowing the verification and/or validation of theoretical models.
›› AVE video extensometer connected to the machines; resolution of 0.5 μm. To determine the axial and transverse deformation without contact (tension, dynamic properties and viscoelasticity) of the samples.

• E1000 Test Instrument: Dynamic systems to offer slow-speed static testing and high-frequency dynamic fatigue testing with hundreds of Hertz capability: Load range: 250 N to 2.0 KN.
• Nano BIONIX MTS: For nanomechanical characterization of biomaterials. Load range to 0.001N to 0.5N.

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U13-E01. MicroCT GE eXplore Locus SP (GE)

MicroCT GE eXplore Locus SP (GE): Computed X-ray Axial Tomograph for the non-destructive study of the microstructure and in vitro tomography: ›› Samples of up to 40 mm in diameter; resolution of 10 μm. ›› Different types of tissues, implants, scaffolds, etc. ›› Complete “section-by-section” imaging with a single rotation (cone beam volumetric technology); excellent image quality and “signal-to-noise” ratio. ›› “Bone Analysis Tool” software provided with: •• BMD (Bone Mineral Density): It calculates the density of minerals in the bone, the bone volume fraction and the mineral content in the bone. •• Anisotropy: It determines the degree of symmetry and the orientation of the trabecular structure. •• Cortical Analysis: It determines “section-by-section” the width, surface area and the BMD value of the cortical bone. •• Stereology: It provides the bone volume fraction, the ratio between the surface of the bone and its volume and the width of the trabecular plate. •• Direct Measures: It determines the local trabecular width of a bone and allows obtaining a visual representation of this local width.

Temporarily OUT OF ORDER

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U12-E14. Avanti J30I ultracentrifuge (Beckman Coulter)

U12-E14. Avanti J30I ultracentrifuge (Beckman Coulter)

Description:
High-speed centrifuge with aluminum rotors at fixed angle – Rotor JA-25.15 (maximum centrifugal force = 74200g, maximum speed = 25000 rpm, capacity for 24 tubes of 15 mL each, total capacity = 360 mL) – Rotor JLA-16.250 (maximum centrifugal force = 38500g, maximum speed = 16000 rpm, capacity for 6 tubes of 250 mL each, total capacity = 1500 mL) – Rotor JA-10 (maximum centrifugal force = 17700g, maximum speed = 10000 rpm, capacity for 6 tubes of 500 mL each, total capacity = 3 L)

Applications:
It allows to centrifuge samples such as emulsions, dispersions, etc at angular velocities up to 25000 rpm (74200 g) at various temperatures (from -20˚C a 40˚C), which can be used for phase separation, stability studies, etc. in colloidal systems and for the separation of cells and proteins.

U12-E14. Avanti J30I ultracentrifuge (Beckman Coulter)
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U12-E13. Turbiscan Lab backscattering stability analyzer (Formulaction)

U12-E13. Turbiscan-lab-backscattering-stability-analyzer-formulaction

Description:
Instrument for the evaluation of colloidal stability. It measures turbidity by multiple light scattering, as a function of time along the vertical axis by both transmitted and backscattered light. It can analyse samples up to 95 vol% with a wide range of particle sizes (10 nm – 1 mm). The samples are placed inside a temperature-controlled chamber, and the measurements can be carried out from room temperature up to 60 ˚C.

Applications:
Allows to study the colloidal stability of emulsions, suspensions and foams and to determine whether there is sedimentation, creaming, flocculation or coalescence.

U12-E13. Turbiscan-lab-backscattering-stability-analyzer-formulaction
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U12-E12. BX51TRF6 Olympus Optical Microscope

U12-E12. BX51TRF6 Olympus Optical Microscope

Description:
Optical microscope that can work with transmitted or reflected light. Different observation methods can be used: bright field, dark field, phase contrast, differential interference contrast, polarized light, and fluorescence. It includes two revolvers to work at short or long distances. Moreover, it is equipped with a digital camera (Olympus DP73) connected to the computer with a software that captures images and videos.

Applications:

Observation of samples at microscale

U12-E12
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U12-E11. Densitometer DMA 4500M (Anton Paar)

U12-E11. DMA -46 densitometer (Anton Paar)

Description:

Instrument for the Determination of the density of liquid samples using Pulsed Excitation. It is a highly precise instrument, measuring density by the method of resonance vibration frequency of a glass capillary.

Applications:

Measurement of the density of liquid samples at controlled temperature

U12-E11. DMA -46 densitometer (Anton Paar)
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