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News U2

News U2

New device could monitor treatments with anticoagulant to achive personalized therapies

  • Researchers from NANBIOSIS units 2 and 4 have designed a biosensor device based on gold nanostructures that precisely detects the presence of the anticoagulant drug from a small blood sample.
  • Treatments with anticoagulants such as Sintrom® (acenocoumarol) face the difficulty of adjusting the drug doses. This device will allow patients to self-regulate the dose.

 

Researchers of NANBIOSIS unit 4  Biodeposition and Biodetection Unit of the Consejo Superior de Investigaciones Científicas (CSIC), the Catalan Institute of Nanoscience and Nanotechnology (ICN2) and CIBER Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) have developed a biosensor device which allows the monitoring of anticoagulants such as Sintrom® (acenocoumarol) to deliver a personalized therapy in which the patient or doctor can adjust the drug dose to achieve the optimal effect.

The ICN2 Nanobiosensors and Bioanalytical Applications (NanoB2A) Group, led by CSIC researcher Prof. Laura Lechuga, Scientific Director of NANBIOSIS U4 (Biodeposition and Biodetection Unit), designed this plasmonic device containing gold nanostructures to which specific bioreceptors can be attached, in order to detect biomarkers in a reproducible and accurate way using a small sample of the patient’s blood and without the need for any prior treatment.

Previously, the Nanobiotechnology for Diagnostics (Nb4D) Group of the CSIC’s Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), led by Prof. M. Pilar Marco, Scientific Director of NANBIOSIS U2 (Custom Antibody Service (CAbS),  produced haptenized proteins (similar to Sintrom®) and polyclonal antibodies specific for this anticoagulant in the U2 of NANBIOSIS. The large experience of U2 unit in the preparation of labeled proteins and production of monoclonal and polyclonal antibodies has resulted in the generation of specific antibodies capable of recognizing Sintrom®. Their usefulness has been demonstrated in a clinical study using an immunoanalytical technique with high sample throughput.

Building on their previous results, the two groups collaborated to develop an automated biosensor device allowing a personalized dose adjustment of this oral anticoagulant. Their results were recently published in Biosensors and Bioelectronics.

As stated by CSIC researcher Prof. Laura Lechuga, “we have studied the optimal conditions to develop this biosensor device and have obtained excellent sensitivity to detect the anticoagulant, which indicates that it is possible to measure very low concentrations of this drug”.

Since it is a quantitative and highly sensitive technology, this device is very suitable for its use in clinical environments, where it could provide a real time follow-up of the medication, or even for patients to manage the medication themselves, a significant breakthrough for people receiving anticoagulants.

Patients suffering from cardiovascular diseases or thromboembolic disorders are usually treated with anticoagulants such as Sintrom® to prevent blood clots formation. The problem is that this treatment entails risks, explain the researchers. “For example, in case the dose is too low, it would not achieve the expected effect and blood clotting may occur; On the other hand, in case the dose is too high, it could cause side effects such as internal bleeding. The right dose depends on many factors such as weight, age, diet and interaction with other medications. ”

 

Reference articles:

Nanoplasmonic biosensor device for the monitoring of acenocoumarol therapeutic drug in plasma Cristina Peláez, M Carmen Estévez, Alejandro Portela, Pablo Salvador, Pilar Marco, Laura M. Lechuga. Biosensors and Bioelectronics, 119, 2018, 149-155 – DOI: 10.1016/j.bios.2018.08.011

Enzyme-linked immunosorbent assays for therapeutic drug monitoring coumarin oral anticoagulants in plasma J.Pablo Salvador, Dolors Tassies, Joan-Carles Reverter, M.-PilarMarco. Analytica Chimica Acta. https://doi.org/10.1016/j.aca.2018.04.042

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Two units of NANBIOSIS in the project selected by La Marató TV3 to fight against bacterial infections

La Marató TV3 in its call for biomedical projects in infectious diseases has selected the project “Uses of molecular analysis of Quorum Sensing with the diagnosis of bacterial infections” of the Nb4D group, led by Mª Pilar Marco, Scientific Director of unit 2 of NANBIOSIS and in which Miriam Royo also participates Scientific Coordinator of unit 3 of NANBIOSIS, both in the IQAC-CSIC of Barcelona

The project of the Nanobiotechnology for Diagnosis (Nb4D) group of the CSIC and the CIBER-BBN has been selected among more than 200 candidates as a project of scientific excellence to be funded in the call for biomedical projects of the Fundació la Marató de TV3 and Catalonia Radio. The project, led in the Nb4D group by Dr. M.-Pilar Marco, Scientific Director of unit 2 of NANBIOSIS and Dr. Lluïsa Vilaplana, will be carried out in collaboration with Dr. M.-Teresa Martín (Hospital Vall d’Hebrón) and aims to study the potential of various molecules of Quorum Sensing as diagnostic tools for bacterial infections. The project, financed with € 227,684.84, also has the collaboration of Dr. Miriam Royo, Scientific Coordinator of Unit 3 of NANBIOSIS, of the Multivalent Systems for Nanomedicine group, also belonging to the CSIC and the CIBER-BBN.

The funds raised in the 2017 edition of the Marató de TV3 and Cataluña Radio will finance 36 biomedical research projects of excellence in infectious diseases to promote the creation of new tools for prevention and diagnosis, as well as more efficient treatments with the aim of earning more and more quality of life for patients.

Link to the news:
http://www.ccma.cat/324/la-marato-impulsa-36-projectes-de-recerca-biomedica-en-malalties-infeccioses/noticia/2884103/

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NANBIOSIS in the Posters presentation in CIBER-BBN ANNUAL CONFERENCE 2018

Last 12 and 13 of November, CIBER-BBN  has celebrated its 12th Annual Conference in Hotel Felipe IV in Valladolid. In poster session participated  NANBIOSIS  itself and some of its units.

  • NANBIOSIS, Infrastructure for the Production & Characterization of Biomaterials, Nanomaterials and medical devices up to preclinical validation. Nanbiosis management team.
  • Divalent cation effects on assembly of histidine-rich protein nanoparticles. López-Laguna, U. Unzueta, O. Conchillo-Solé, A. Sánchez-Chardi, M. Pesarrodona, O. Cano-Garrido, E. Voltà, L. Sánchez-García, N. Serna, P. Saccardo, R. Mangues, A. Villaverde, E. Vázquez (NBT-UAB) (U1 -U18)
  • Antimicrobial peptides (AMPs) anchored on the surface of contact lenses to prevent corneal infections. Emiliano Salvagni, Clara García, Àngels Manresa, Carlos Rodríguez, María José García-Celma, Claudia Müller-Sánchez, Manuel Reina, Jordi Esquena (QCI-CSIC) (U12)
  • Preliminary studies on ultrasound characterization of perfluorocarbon-loaded polymeric nanocapsules. Gabriela Calderó, Marie Pierre Krafft, Da Shi, María José García-Celma, Conxita Solans, Carlos Rodríguez-Abreu (QCI-CSIC) (U12)
  • Multiplexed analytical platforms based on the use of antibodies for monitoring pollutants in marine environment samples. -Pablo Salvador, Ana Sanchís, Klaudia Kooper, Andrea Miti, M.-Pilar Marco (Nb4D-IQAC-CSIC) (U2)
  • In vitrodiagnostics of neurological disorders through the immunochemical detection of kynurenine. Montserrat Rodríguez Núñez, Ana Sanchís, Lluïsa Vilaplana, Roger Galve, M.-Pilar Marco (Nb4D-IQAC-CSIC) (U2)
  • Custom Antibody Service: From the molecule to the bioassay. Núria Pascual, Ana González-Gomzález, M.-Pilar Marco (Nb4D-IQAC-CSIC) (U2)
  • Hyaluronic acid enhances insulin release of microencapsulated pancreatic progenitors differentiated from mesenchymal stem cells. Alberto Cañibano-Hernández, Laura Sáenz del Burgo, Albert Espona-Noguera, Gorka Orive, Rosa María Hernández, Jesús Ciriza, Jose Luis Pedraz (NANOBIOCEL) (U10 )
  • Type 1 Diabetes Mellitus reversal via implantation of magnetically purified microencapsulated pseudoislets. Albert Espona-Noguera, J. Etxebarria-Elezgarai, L. Saenz del Burgo, A. Cañibano-Hernández, H. Gurruchaga, Gorka Orive, Rosa M. Hernández, F. Benito-Lopez, J. Ciriza, L. Basabe-Desmonts and J.L. Pedraz (NANOBIOCEL) (U10 )
  • Unraveling the immune system participation in therapy response in GL261 glioblastoma: correlation with MRSI-based molecular imaging techniques. Calero, N. Arias-Ramos, R. Rabanal, M. Pumarola, C. Arús, A.P. Candiota (GABRMN-UAB) (U25)
  • Dual T1/T2 nanoscale coordination polymers as novel contrast agents for MRI: a preclinical study for brain tumor. Suárez-García, N. Arias-Ramos, C. Frias, A.P. Candiota, C. Arús, J. Lorenzo, D. Ruiz-Molina, F. Novio (GABRMN-UAB) (U25)
  • Impact of high glucose levels and ketoacidosis associated to diabetic complications on bacterial growth and attachment to Ti6Al4V. Miguel A. Pacha-Olivenza, María Fernández-Grajera, Daniel Romero-Guzmán, M. Luisa González-Martín (AM-UEX) (U16)
  • Preparation, characterization and bacterial performance on PLDA and PLDA 10% Mg films. Verónica Luque-Agudo, Daniel Romero-Guzmán, Coronada Fernández-Calderón, Miguel A. Pacha-Olivenza, María Fernández-Grajera, Margarita Hierro-Oliva, M. Luisa Navarro-Pérez, Ciro Pérez-Giraldo, M. Luisa González-Martín, Amparo M. Gallardo-Moreno (AM-UEX) (U16)
  • A wavelet-based approach for automatic diagnosis of strict left bundle branch block. Alba Martín-Yebra, Juan Pablo Martínez (BSICoS) (U27)
  • ECG-derived respiration in atrial fibrillation. Spyridon Kontaxis, Jesús Lázaro, Valentina D. A. Corino, Frida Sandberg, Raquel Bailón, Pablo Laguna, and Leif Sörnmo (BSICoS) (U27)
  • Development of highly metastatic, CXCR4-overexpressing, colorectal cancer models. Rita Sala, Alberto Gallardo, Ugutz Unzueta, Patricia Álamo, Carmen Cabrera, Esperanza Medina, Isolda Casanova, Irene Arroyo, Aïda Falgás, Carlos Navas, Manuel Trías, Antonio Villaverde, Esther Vázquez, Ramón Mangues, Mª Virtudes Céspedes (GOA-HSPau) (U18)
  • PATHGATE: Oligonucleotide-gated sensing nanodevices for pathogen detection. Luis Pla, Angela Ribes, M. Angeles Tormo-Mas, Javier Pemán, Félix Sancenón, Elena Aznar, Ramón Martínez-Máñez and Sara Santiago-Felipe (IQMA-IDM-UPV) (U26)
  • Towards chemical communication between abiotic nanoparticles and living systems. Beatriz de Luis, Paola Ricón, Cristina de la Torre, Antoni LLopis, Jose Gadea, Jose R. Murguía, Félix Sancenón, Ramón Martínez-Máñez, Elena Aznar (IQMA-IDM-UPV) (U26)
  • Development  and  in vitro evaluation  of  antimicrobial  polymer  coatings  for the prophylactic treatment of hernia repair materials. Bárbara Pérez-Köhler, Selma Benito, Mar Fernández-Gutiérrez, Gemma Pascual, Marta Rodríguez, Verónica Gómez-Gil, Francisca García-Moreno, Julio San Román, Juan Manuel Bellón (GITBIT-UAH) (U17)
  • Experimental study of the application of a new bone cement loaded with broad spectrum antibiotics for the treatment of bone infection. Joaquín García, Galo Azuara, Blanca Ibarra, Miguel A. Ortega, Ángel Asúnsolo, Blanca Vázquez, Julio San Román, Julia Buján, Basilio De la Torre, Natalio García-Honduvilla (GITBIT-UAH) (U17)
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Nanoplasmonic biosensor device for the monitoring of acenocoumarol therapeutic drug in plasma

As a result of the collaboration carried aout by two units of NANBIOSIS, U4. Biodeposition and Biodetection Unit and U2. Custom Antibody Service (CAbS), it has been developed a compact and simple nanoplasmonic sensing device based on gold nanodisks for the rapid monitoring of acenocoumarol, using highly specific polyclonal antibodies produced against the drug Acenocoumarol (Sintrom®) which implies:

  • A label-free nanoplasmonic device for the rapid monitoring of acenocoumarol in plasmaD.
  • Direct quantification in real time requiring low sample volume is achieved.
  • Non-specific interferences from plasma are minimized using the developed methodology.
  • Excellent accuracy has been observed measuring blind plasma samples.
  • Potential to be implemented as a POC device in decentralized settings.

 

The research details have been published in the article authored by E. CristinaPeláezM.-CarmenEstevezAlejandroPortelaJ.-PabloSalvadorM.-PilarMarco and Laura M.Lechuga, “Nanoplasmonic biosensor device for the monitoring of acenocoumarol therapeutic drug in plasma, Biosensors and Bioelectronic, 119, 2018, 149-155 – DOI: https://doi.org/10.1016/j.bios.2018.08.011

 

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Interferometric nanoimmunosensor for label-free and real-time monitoring of Irgarol 1051 in seawater

As a result of a collaboration in the context of the EC-FP7 program OCEAN 2013, NANBIOSIS Unit 2 Custom Antibody Service (CAbS) and  Unit 4. Biodeposition and Biodetection,  the research groups coordinating this NANBIOSIS Units have recently published an article titled “Interferometric nanoimmunosensor for label-free and real-time monitoring of Irgarol 1051 in seawater” in the scientific magzine  iosensors and Bioelectronics.

The CIBER-BBN-ICN2 group  Nanobiosensors and Bioanalytical Applications, led by Prof. Laura Lechuga has developed the immunosensor that is described in the article using immunoreactive, produced in the group of  Nanobiotechnology for Diagnostics Group (Nb4D), led by Prof. Pilar Marco, with the collaboration of CAbS, for Irgarol 1051. This compound is an alguicide used as an additive in the paintings of ships. Over time, the compose is being released  into the marine environment causing a risk to the ecosystem and to the health of the population.

In the article, an interferometric nanobiosensor for the specific and label-free detection of the pollutant Irgarol 1051 directly in seawater has been settled. Due to the low molecular weight of Irgarol pollutant and its expected low concentration in seawater, the sensor is based on a competitive inhibition immunoassay. Parameters as surface biofunctionalization, concentration of the selective antibody and regeneration conditions have been carefully evaluated. The optimized immunosensor shows a limit of detection of only 3 ng/L, well below the 16 ng/L set by the EU as the maximum allowable concentration in seawater. It can properly operate during 30 assay-regeneration cycles using the same sensor biosurface and with a time-to-result of only 20 min for each cycle. Moreover, the interferometric nanosensor is able to directly detect low concentrations of Irgarol 1051 in seawater without requiring sample pre-treatments and without showing any background signal due to sea matrix effect.

Article of referencehttps://doi.org/10.1016/j.bios.2018.05.044

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NANBIOSIS U2 incorporates a new Nitrogen tank to store cells

NANBIOSIS U2. Custom Antibody Service (CAbS), is incorporating new equipment as a result of its participation in the project FICTS1420-27, selected by the MINECO for co-financing by the FEDER Program in ICTS 2014-2020.

Recently the purchase and installation and set-up of a new nitrogen tank to store cells has taken place. The storage of cell lines of monoclonal antibody-producing hybridomas is a process in which the cells are conserved when they are cooled to temperatures typically at -196 ° C in liquid Nitrogen tanks. With temperatures below -130 ° C the activity Biological ceases and storage at these temperatures is vital to keep the material unaltered. The increasing activity of the NANBIOSIS U2 the makes necesary to incorporate a new tank of liquid Nitrogen for the storage of new hybridoma lines.

For further information:

 

Liquid nitrogen tank
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Agreements signed with MINECO for the allocation of FEDER funds for NANBIOSIS ICTS

In the framework of the FEDER Program in ICTS 2014-2020, several projects related to the ICTS NANBIOSIS have been selected by the MINECO for co-financing with FEDER funds of the European Regional Development Funds program.

An agreement has been signed between MINECO and CIBER (partner of NANBIOSIS for the co-financing of the Project: “Purchase, installation and set-up of production and characterization equipment to complement the Units: U3-Synthesis of Peptides Unit, U18-Nanotoxicology and U20- In Vivo Experimental Platform”. The total budget of the project amounts to € 307,566.16, with 50% financing with FEDER Funds.

Also CSIC (The State Agency Superior Council of Scientific Investigations), institution that houses some of the NANBIOSIS units,  as distributed ICTS,  has signed an agreement with MINECO for the co-financing of the Project: “Purchase and installation and set-up of equipment and production and characterization laboratories to complement the units U2-Production of antibodies, U4-Biodeposition and biosensing, U6-Processing of biomaterials and U8-Micro, nanotechnology. The total budget of the project amounts to € 312.800,00 €, with 50% financing with FEDER Funds.

These two projects aim to increase the quantity and quality of the services offered by th implied units, with the objetive of positioning them as national and international benchmark in their respective fields of application. As a consequence, an increase in the performance (number of services and number of users) of each unit is expected, especially from companies (pharmaceutical and small biotechnology).

CSIC and CIBER are processing the necessary contracting procedures for the execution of these projects.

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How to validate your favourite antibodies?

Are you interested in learning how to validate your favourite antibodies?

Do not miss the opportunity to attend the 4th EuroMAbNet Antibody Validation Workshop!

The Antibody Validation Workshop brings together highly specialized scientists in monoclonal antibody (mAbs) technology with PhD students and young scientists who are using antibodies in their research projects. The workshop will provide a set of criteria and recommendations that will help you to select the most effective mAbs from those available in the market and provide the strategic guidance needed to perform any essential antibody validation. This includes verifying antibody specificity, selectivity, sensitivity and reproducibility.

Nuria Pascual, Scientific Coordinator of NANBIOSIS U2, Custom Antibody Service (CAbS), is member of EUROMABNET and member of  organizing committee of the antibody validation workshop

Click  here to see the program of the, 4th EuroMAbNet Antibody Validation Workshop, 1st of July 2018, University of Ghent’s Congress Center, Ghent , Belgium. The 4th EuroMAbNet Antibody Validation Workshop will be held as a satellite event within the Core Technologies for Life Sciences (CTLS) meeting 2018.

Register  here before the 20th of June

 

  • Registration fee: 80€  (Coffee breaks and lunches are included in the registration)
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Designing nanomedicines

Next May 3, will take place in Barcelona, at the Academy of Medical and Health Sciences of Catalonia and the Balearic Island, an informative session and debate about Nanotecnology and Health “Designing Nanomedicines” in wich Prof. Antonio Villaverde, Strategy Director of NANBIOSIS U1. Protein Production Platform (PPP) will give a talk on “Nanobiotechnological approaches to cancer medicine“. Prof. Jesús Martínez de la Fuente, (ICMAB-CSIC / Universidad de Zaragoza – CIBER-BBN) will speak about “the nanorevolution: towards a healthier world“.

The organization of the even has been in charge of Pilar Marco, Scientific Director of NANBIOSIS U2  Custom Antibody Service (CAbS), as a member of the board of directors of the Catalan Society of Medical Biotechnology.

Programe

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Unit 2 Nanbiosis have signed an agreement with a Belgian Enterprise.

U2 unit of Nanbiosis (Custom antibody service) has recently signed a 3-year contract with UNISENSOR S.A. (Liege, Belgium) for producing on a high scale a collection of immunoreagents used in their diagnostic kits.

UNISENSOR S.A. is a SME biotech company that develops on site diagnostic systems for food safety. Unit 2 of NANBIOSIS, coordinated by Nb4D-CSIC holds a research collaboration for more than 7 years developing new bioproducts and transferring knowledge to UNISENSOR S.A. As result of the collaboration several diagnostic kits are nowadays in the market, consequently this contract has been signed with the aim to supply UNISENSOR S.A. with immunoreagents for their diagnostic products. The biomolecules produced by CAbS are completely characterized and respond to quality control criteria required by UNISENSOR S.A.

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