Preclinical validation characterization - Equipment - Nanbiosis

U16-E02. UVISEL ellipsometer (Horiba JovinYvon)

UVISEL ellipsometer (Horiba JovinYvon), range 190-2100 nm, based on phase modulation with cell for liquid media:

•• Measurement of thicknesses from 0.1 nm to 30 μm.
•• Measurement of thickness of various superimposed layers.
•• Simultaneous measurement of the proportion of various different materials present on a surface.
•• Estimation of the porosity as percentage of pores vs. deposited material.
•• Measurement of roughness of coating between 2.5 and 15 nm.

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U16-E01. TAM III isothermal nanocalorimeter system. (TA Instruments)

TAM III isothermal nanocalorimeter system including options for titration and perfusion. Also solution quasi-adiabatic calorimetry is available. (TA Instruments). Thermostatic bath: •• Working temperatures between 15 and 150 ºC •• Temperature scan < 2 ºC/h •• Stability: /- 10 μK on short timescales and /- 100 μK drift over 24 hours Calorimetric response: •• Precision better than 100 nW •• Reproducibility better than 1% •• Stability: < 10 nW on short timescales and < 40 nW drift over 24 hours Solution: •• Maximum temperature 80ºC •• Quasi-adiabatic mode of operation

Temporarily OUT OF ORDER

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U15-E02. T1 and T2 Nuclear Magnetic Resonance Relaxometry

T1 and T2 Nuclear Magnetic Resonance Relaxometry:

Stelar SmarTRACER (Italy) + Bruker (Germany) 2 T electromagnet. Fast Field Cycling designed to measure longitudinal nuclear magnetic relaxation as a function of the magnetic field intensity. Measurements of the longitudinal (T1) and transverse (T2) time constants as a function of the Larmor frequency.
•• Measurement range: continuous measurement from 10 kHz (almost null field) to 10 MHz (0.25T) to obtain T1.
•• Measurement range: measurement from 10 MHz (0.25T) to 80MHz (1.9T) at desired intervals to obtain T1 and T2.
•• Inhomogeneity lower than 150 PPM.
•• Main pulse sequences implemented with the possibility of modifying parameters to the design and programming of new sequences.
•• Temperature control from -120°C to +140°C with accuracy and stability of 0.1°C.

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U15-E01. Alternating Gradient Magnetometer

Alternating Gradient Magnetometer:

MicroMag M2900-4 AGM (Princeton Measurements Corporation, USA) for the magnetic and mechanical characterization of nanoparticles in various different media:
•• Magnetic Moment Range: 1nA.m² to 5mA.m² full scale (1μemu to 5emu).
•• Resolution: 0,005% of full scale with 60% overrange capability.
•• Speed of measurement: 100 ms/point.
•• High sensitivity: 10 pA.m² (10 nemu) of standard deviation at room temperature and with 1 second of averaging time.
•• High performance with samples of very small magnetization and dimensions (few nanometers).
•• The AGM can accommodate a large range of samples with widely different properties.

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U14-E01. Cell culture rooms

Cell Culture Room 1:
Vertical Laminar Flow Cabinet Herasafe® (Formimetal).
CO2 incubator Heracell 150 (Thermo)
Nomarski phase inverted microscope and fluorescencia con cámara (Nikon)
Camera Color 1CCD (Nikon)
Cooled Camera black and white (Nikon)
Vacuum Pump ME4 (Vacuubrand)
Cell culture water Baths (Controltecnica)

Cell Culture Room 2:
Vertical Laminar Flow Cabinet Herasafe® (Formimetal)
CO2 incubator Heracell 150 (Thermo)
CO2 incubator Heracell 150 with CO2 control (Thermo)
phases inverted microscope with fluorescence (NIKON)
Vacuum Pump ME4 (Vacuubrand)
Cell Counter (Invitrogen)

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U14-E02. Molecular biology

Molecular Biology
Fume Hood (Formimetal)
Stereo microscope with camera and digital image set (NIKON)
light source (for the glass) (NIKON)
Refrigerated centrifuge for microtubs Biofuge® Pico (HERAEUS)
Digital circulation thermostatic bath (GRANT)
Thermostatic bath (for cell cultures temperar medios) (MEDIGEN))
Pipette controller pipette Powerpette-plus (IZASA)
Pipettes de volumen variable continuo with accessories (RAININ)
Vertical Freezer -20ºC (FIOCHETTI)
Freezer +2/+8ºC (CLASS ELECTRONIC)
Laboratory vertical freezer Herfreeze ®HFU-486-Basic (-80ºC) (HERAEUS)
Crioconservtion system with accessories
Liquid Nitrogen tank (taylor wharton)
Thermocycler (THERMO)
Sterilization incubator (Modelo UT ) (HERAEUS)
G-Box Translumitator and accessories (PC, Sofware…) (SYNGENE)
Electrophoresis system (IZASA)
Electrophoresis vertical jar
Electrophoresis horizontal (big) jar
Electrophoresis horizontal (small) jar
Rotary shaker with incubation (HEIDOLPH)
Magnetic stirrer with heating (ARE)
Analytical balance 210gr, 0,01 gr. (ACCULAB)
Precision analytical balance (OHAUS)
Ultrasonic water bath (SELECTA)
Crushed ice machine (Schottman)
Microwave (LG)
Pipette controller (POWERPETTE PLUS)
Thermoblock (Labolan)
Vortex 230 V, EU (VWR)
Freezer +4ºC/-20ºC
UV mini-1240 Spectrophotometer (SHIMADZU)
ELISA plate reader, Synergy MX (BIOTEK)
Flow cytometry FACSCalibur (BD)
pH-Meter (Symphony)
i-Blot (Invitrogen)
Autoclave

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U13-E02. Machines for mechanical tests

Machines for mechanical tests:

• Instron MicroTester 5848 and 5548: electromechanical machines for displacement or load control tests:

•• load range from 1mN to 2kN at full scale.
•• load cells of:+/- 5, 50, 500 and 1000N.
•• synchronous data capture in all the channels up to
500 Hz.
• Biaxial Instron MicroTester 8874: axial-torsional, servohydraulic testing machine:
•• load capacity of 25 kN and 100 Nm.
•• fatigue tests at a low number of cycles.

›› Software for data control and analysis.
›› Accessories and tools especially designed by the group, which allow a wide flexibility in designing tests, allowing the verification and/or validation of theoretical models.
›› AVE video extensometer connected to the machines; resolution of 0.5 μm. To determine the axial and transverse deformation without contact (tension, dynamic properties and viscoelasticity) of the samples.

• E1000 Test Instrument: Dynamic systems to offer slow-speed static testing and high-frequency dynamic fatigue testing with hundreds of Hertz capability: Load range: 250 N to 2.0 KN.
• Nano BIONIX MTS: For nanomechanical characterization of biomaterials. Load range to 0.001N to 0.5N.

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U13-E01. MicroCT GE eXplore Locus SP (GE)

MicroCT GE eXplore Locus SP (GE): Computed X-ray Axial Tomograph for the non-destructive study of the microstructure and in vitro tomography: ›› Samples of up to 40 mm in diameter; resolution of 10 μm. ›› Different types of tissues, implants, scaffolds, etc. ›› Complete “section-by-section” imaging with a single rotation (cone beam volumetric technology); excellent image quality and “signal-to-noise” ratio. ›› “Bone Analysis Tool” software provided with: •• BMD (Bone Mineral Density): It calculates the density of minerals in the bone, the bone volume fraction and the mineral content in the bone. •• Anisotropy: It determines the degree of symmetry and the orientation of the trabecular structure. •• Cortical Analysis: It determines “section-by-section” the width, surface area and the BMD value of the cortical bone. •• Stereology: It provides the bone volume fraction, the ratio between the surface of the bone and its volume and the width of the trabecular plate. •• Direct Measures: It determines the local trabecular width of a bone and allows obtaining a visual representation of this local width.

Temporarily OUT OF ORDER

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U12-E14. Avanti J30I ultracentrifuge (Beckman Coulter)

Description:
High-speed centrifuge with aluminum rotors at fixed angle – Rotor JA-25.15 (maximum centrifugal force = 74200g, maximum speed = 25000 rpm, capacity for 24 tubes of 15 mL each, total capacity = 360 mL) – Rotor JLA-16.250 (maximum centrifugal force = 38500g, maximum speed = 16000 rpm, capacity for 6 tubes of 250 mL each, total capacity = 1500 mL) – Rotor JA-10 (maximum centrifugal force = 17700g, maximum speed = 10000 rpm, capacity for 6 tubes of 500 mL each, total capacity = 3 L)

Applications:
It allows to centrifuge samples such as emulsions, dispersions, etc at angular velocities up to 25000 rpm (74200 g) at various temperatures (from -20˚C a 40˚C), which can be used for phase separation, stability studies, etc. in colloidal systems and for the separation of cells and proteins.

U12-E14. Avanti J30I ultracentrifuge (Beckman Coulter)

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U12-E13. Turbiscan Lab backscattering stability analyzer (Formulaction)

U12-E13. Turbiscan-lab-backscattering-stability-analyzer-formulaction

Description:
Instrument for the evaluation of colloidal stability. It measures turbidity by multiple light scattering, as a function of time along the vertical axis by both transmitted and backscattered light. It can analyse samples up to 95 vol% with a wide range of particle sizes (10 nm – 1 mm). The samples are placed inside a temperature-controlled chamber, and the measurements can be carried out from room temperature up to 60 ˚C.

Applications:
Allows to study the colloidal stability of emulsions, suspensions and foams and to determine whether there is sedimentation, creaming, flocculation or coalescence.

U12-E13. Turbiscan-lab-backscattering-stability-analyzer-formulaction

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Preclinical validation characterization - Equipment

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