+34 620 10 75 37info@nanbiosis.com

U6. Biomaterial Processing and Nanostructuring Unit

U6. Biomaterial Processing and Nanostructuring Unit

You are here : Home / U6. Biomaterial Processing and Nanostructuring Unit (Page 3)

U6-S05

Freeze dryer

Freeze drying (also known as lyophilization) is a water (or other solvents) removal process typically used to preserve materials, with the goal of extending their shelf life or reducing its weight. Freeze drying works by freezing the material, then reducing the pressure and adding heat to allow the frozen water in the material to change directly to a vapor (sublimation).

Freeze drying occurs in three phases:

  1. Freezing: Freezing can be done in a freezer, a chilled bath (shell freezer) or on a shelf in the freeze dryer. Cooling the material below its triple point ensures that sublimation, rather than melting, will occur. This preserves its physical form.
  2. Primary Drying: Freeze drying’s second phase is primary drying (sublimation), in which the pressure is lowered and heat is added to the material in order for the water to sublimate. About 95% of the water in the material is removed in this phase. Primary drying can be a slow process.
  3. Secondary Drying: Freeze drying’s final phase is secondary drying (adsorption), during which the ionically-bound water molecules are removed. Most materials can be dried to 1-5% residual moisture.

Customer benefits

  • Solvent removal typically used to preserve materials, with the goal of extending their shelf life or reducing its weight.
  • The design of the equipment offers the best performance in the smallest possible space.
  • Equipment suitable for laboratories: compact and easy to install.
  • Technical reliability and excellent performance.
  • Ease of use: touch screen user interface.

Target customer

  • Pharmaceutical industry
  • Food Industry
  • Chemical industry
  • Materials research centers
• Read More

U6-S06.

Pulse-free microfluidic system

Our device offers a pulse-free miniaturized platform designed to manipulate and control small volumes of fluids at the microscale, ranging from microliters to picoliters. The compact size, reduced sample consumption, faster reaction times, and potential for automation make microfluidic devices advantageous for a wide range of scientific and biomedical purposes.

Customer benefits

Offers precise handling of fluids within microchannels or microstructures, enabling various applications such as chemical analysis, biological assays, drug delivery, and point-of-care diagnostics.

Target customer

  • Chemical and biochemical companies.
  • Biology and chemistry research groups.
• Read More

U6-S07.

Interactions of two biomolecules with respect to binding kinetics and affinity by surface plasmon resonance (SPR).

Technically speaking, SPR refers to an optical phenomenon that enables monitoring of changes in refractive index via a quantum mechanical principle.

In a traditional SPR experiment:

  • A target is immobilized or captured onto a surface known as a sensor chip.
  • A pump is used to flow analytes over the sensor chip.
  • An optical measurement system captures changes occurring on the surface of the sensor chip.
  • Software plots time-dependent responses in the form of a graph called a sensorgram.

Customer benefits

With SPR, you can determine the rates and affinity of interactions between biomolecules and answer multiple questions using a single instrument.
SPR is the gold standard for analyzing biomolecules, providing:
High quality kinetics (association and dissociation constants, plus equilibrium)

  • Real-time data acquisition.
  • Label-free analysis.
  • Faster, automated experiments.
  • Lower sample consumption.

SPR’s flexibility lets you study biomolecular interactions in a wide variety of analytes, from small molecules in drug discovery to peptides, proteins, DNA, viruses, and even whole cells. SPR allows for:

  • Simple yes/no binding
  • Equilibrium studies
  • Complex kinetic analyses
  • Thermodynamic analysis
  • Concentration determination

Target customer

  • Pharmaceutical industry
  • Food Industry
  • Chemical industry
  • Materials research centers

• Read More

U6-S08.

Tangential flow filtration

Tangential flow filtration (TFF) is a process of separation widely used in bio-pharmaceutical and food industries. It is different from other filtration systems in that the fluid is passed parallel to the filter, rather than being pushed through a membrane perpendicularly which can clog the filter media. This method is preferred for its continuous filtration and reproducible performance. The particles that pass through the membrane, the permeate, are put off to the side, while the rest, the retentate, is recycled back to the feed.

Customer benefits

Tangential flow filtration is used in the following processes:

  • Concentration: Increases the concentration of a solution by removing fluids while keeping the solute molecules. This process is done by selecting a filter significantly smaller than the solute molecules to allow for a higher retention of solute molecules.
  • Diafiltration: The separation of small and large particles, leaving the smaller particles behind without altering the overall concentration.

Target customer

  • Pharmaceutical industry
  • Food Industry
  • Chemical industry
  • Materials research centers

References

M.Köber, et al., J.C.I.S 2023, 631, 202-211

• Read More

U6-S09.

Multimode plate reader

The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable high-performance detection solutions empowered by monochromator- or filter-based technologies. The six new tailored configurations provide excellence in ELISA assays, nucleic acid quantifications, reporter assay technologies, and drug discovery assays including HTRF® and fluorescence polarization.
Dual-mode plate reader with monochromator-based optics for absorbance and sensitive fluorescence (top and bottom reading) applications. Your adjustable tool, even for low concentration nucleic acid and protein quantification.
The Infinite M Nano+ has an excitation monochromator optimized for wavelength accuracy and precision, ensuring excellent performance for every absorbance and fluorescence assay.Engineered for absorbance and fluorescence measurements, the system’s highly sensitive Quad4 Monochromators™ minimize stray light, delivering exceptional flexibility with sensitivity levels close to comparatively priced filter-based instruments.

Customer benefits

Intuitive, workflow-oriented software (i-control) which allows you to create a workflow for each application, using ‘drag and drop’ processing steps to generate your assay protocol, which can be saved for future use.

Highlights:

  • Real-time export data
  • Extended dynamic range
  • Automated z-focusing

Key applications:

  • Absorbance-based DNA/RNA quantification and purity checks
  • Fluorescence-based DNA/RNA quantification (PicoGreen, RiboGreen®)
  • Absorbance-based protein quantification (BCA, Bradford, Lowry, etc.)
  • Fluorescence-based protein quantification (eg. NanoOrange®)
  • Absorbance- and fluorescence-based ELISAs600 nm growth curves (bacteria, yeast)
  • Enzyme kineticsCompound characterization

Target customer

  • Pharmaceutical industry
  • Food Industry
  • Chemical industry
  • Materials research centers

• Read More

U6-S02. High-pressure phase analysis – solubility, emulsification (Remote) OUTSTANDING

High-pressure phase analysis – solubility, emulsification.

This phase equilibria unit is built for the measurement and detection of phase equilibria and phase transitions by optical means.
The mixture of solute and solvent gas is agitated by the magnetic stirrer. Whenever samples are drawn from the top or the bottom connection in the cell, the directly connected counterbalance piston moves towards the centre of the cell, this keeping the pressure in the measuring cell constant even during the sampling operation. No need to add additional solvent gas, which would change mass ratio and temperature and consequently result in a disturbed equilibrium.

Customer benefits

Phase equilibria cell:
• Capacity: 29-55 mL (depending on piston position)
• Operating pressure max.: 200 Bar
• Operating temperature max.: 150 °C

Counterbalance piston to maintain constant pressure during sampling operation
• Optical windows: 2x ø28mm (sapphire)
• Optical path length: 58 mm

Double wall heating jacket for heating with external thermostat.
High-pressure thermocouple type K (inner temperature)

Target customer

  • Pharmaceutical industry
  • Food Industry
  • Chemical industry
  • Materials research centers

References

N-Grimaldi, et al. ACS Nano 2017, 11, 10774-10784

• Read More

U6-S01. Use of High-pressure laboratory-scale plant

Use of High-pressure laboratory-scale plant

with 50, 100 and 300 m reactors for the processing of biomaterials. Processing of cytotoxic compounds when required.

Preparation of soft molecular materials with controlled structure at supramolecular, micro- and nanoscopic level, using one-step methodologies based on green compressed fluids (i.e. compressed and supercritical CO2).Micro- and nanoparticulate single compounds with high supramolecular homogeneity (i.e., pure polymorphic phases, materials with single polymer folding, etc.).

  • Particulate polymeric matrix uniformly loaded with active compounds (therapeutics, cosmetic ingredients, catalyst, pigments, and dyes, etc.)
  • Dispersed systems (suspensions, liposomes, emulsions, vesicles) with narrow particle size distribution and high morphological homogeneity.
  • Porous materials, either crystalline or amorphous, with defined porosity and porous size.

Customer benefits

Lab-scale high pressure systems, based on a 50 mL, a 100 mL and a 300 mL stirred high pressure autoclaves equipped with pumps for the supply of compressed fluids and liquid solutions. The high-pressure systems can also optionally be equipped with several filters, manometers, thermocouples, and back pressure regulators. The maximum operative pressure is 23 MPa and the maximum operative temperature is 200 °C.
The 300 mL system is also equipped with a mass flowmeter, and a data acquisition system.
All the plants have been designed for micro- and nanostructuring molecular and soft materials.

Target customer

  • Pharmaceutical industry
  • Food Industry
  • Chemical industry
  • Materials research centers

References

• Read More

U6-E05. Nanosight NS-300 for Nanoparticle Tracking Analysis by fluorescence mode

Nanosight NS300; Malvern Instruments

Descripción: Automated analysis of the size distribution and concentration

The Malvern NanoSight NS300 uses the technology of Nanoparticle Tracking Analysis (NTA). This unique technology utilizes the properties of both light scattering and Brownian motion in order to obtain the size distribution and concentration measurement of particles in liquid suspension. A laser beam is passed through the sample chamber, and the particles in suspension in the path of this beam scatter light in such a manner that they can easily be visualized via 20x magnification microscope onto which is mounted a camera. The camera operates at 30 frames per second (fps), capturing a video file of the particles moving under Brownian motion. The software tracks many particles individually and using the Stokes-Einstein equation calculates their hydrodynamic diameters.

Location: Laboratory SCF.  (MATGAS)

More info:  http://www.malvern.com/en/products/product-range/nanosight-range/nanosight-ns300/default.aspx

Especificaciones técnicas:

Wavelength: 405nm (violet), 488nm (blue), 532nm (green) or 642nm (red).

Temperature control range: 5°C below ambient to 55°C.

Stage: Fixed stage.

Focus: Computer controlled motorized focus.

Camera:

Fluorescence: Motorized 6 place filter wheel with choice of filters.

Particle size: 10nm to 2000nm.

Concentration range: 106to 109 particles per mL.

• Read More

U6-S14. Quantitative studies of biomolecular interactions by calorimetric measurements (On-site) OUTSTANDING

Quantitative studies of biomolecular interactions by calorimetric measurements

Isothermal Titration Calorimetry (ITC) is a thermodynamic technique that directly measures the heat released or absorbed during a biomolecular binding event (protein-small molecule, protein-protein, target-drug, enzyme-inhibitor, antibody-antigen, protein-DNA, protein-lipid, small molecule-small molecule). Measurement of this heat allows accurate determination of binding constants (KB), reaction stoichiometry (n), enthalpy (ΔH) and entropy (ΔS), thereby providing a complete thermodynamic profile of the molecular interaction in a single experiment. Because ITC goes beyond binding affinities and can elucidate the mechanism of the molecular interaction, it has become the method of choice for characterizing biomolecular interactions.
The equipment used for this purpose is VP-ITC (GE HealthCare-Microcal).

Customer benefits

Applications range goes from drug design to fundamental research, such as understanding and regulating signal transduction pathways. These systems provide direct marker-free and in-solution measurement of binding affinity and thermodynamic parameters in a single experiment. They have high sensitivity, low sample consumption and automation options to minimise handling time.

Target customer

  • Biochemical and Pharmaceutical companies.
  • Biology and biochemistry research groups.

Additional information

M.Köber, et al., Journal of Colloid and Interface Science 631 (2023) 202–211. DOI: 10.1016/j.jcis.2022.10.104

Compra al mejor precio MALVERN MicroCal VP-ITC | Bimedis

• Read More