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Nanbiosis

U2. Custom Antibody Service Unit (CAbS)

U2 CAbS-hybridoma clone
Ciber bbn

U2. Custom Antibody Service (CAbS)

  • Scientific Director: Prof. Pilar Marco pilar.marco@cid.csic.es
  • Scientific Coordinator: Dra. Nuria Pascual npdqob@cid.csic.es
  • Entities: Institute of Avanced Chemistry of Catalonia (IQAC) & Consejo Superior de Investigaciones Científicas (CSIC)
  • Address: Jordi Girona, 18-26, 088034, Barcelona, Spain
  • Phone: +34 934 006 100
  • Web: IQAC- CSIC

IQAC CSIC

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Description

The facility is located in the Institute of Advanced Chemistry of Catalonia (IQAC-CSIC; Barcelona). It is equipped with a laboratory for the development and production of monoclonal antibodies. The laboratory is fully equipped for obtaining, selecting and storing monoclonal antibody producing hybridomas. In addition, the facility has infrastructure for the production of polyclonal antibodies, as well as laboratories for the synthesis of bioconjugates, haptens, the purification of antibodies and the performance of immunoassays. In addition to the above mentioned equipment, the IQAC-CSIC provides animal husbandry facilities and a unit for the synthesis of organic molecules.

The equipment consists in an inverted phase contrast microscope with a camera, two Class II laminar flow biological safety cabinets, two CO2 incubators, a bioreactor for hybridoma culture, and tangential filtration unit for purification of the antibodies produced in the culture. Among the items of available equipment it should be highlighted a spotter for selection of high antibody producing cell lines and an automated ELISA microplate washer an ELISA reader, two AKTA Prime Liquid chromatography systems for antibody purification, plus standard equipment (centrifuges, fridges, liquid nitrogen containers, autoclave, baths, and vacuum equipment) and all the necessary routine laboratory apparatus.Cabs also has access to a fully equipped chemistry laboratory for the synthesis of haptens and immunoreagents.

News U2

17 Apr

Unit 2 Nanbiosis have signed an agreement with a Belgian Enterprise.

U2 unit of Nanbiosis (Custom antibody service) has recently signed a 3-year contract with UNISENSOR S.A. (Liege, Belgium) for producing on a high scale a collection of immunoreagents used in their diagnostic kits. UNISENSOR S.A. is a SME biotech company that develops on site diagnostic systems for food safety. Unit 2 of NANBIOSIS, coordinated by Nb4D-CSIC holds a research collaboration for more than 7 years developing new bioproducts and transferring knowledge to UNISENSOR S.A. As result of the collaboration several diagnostic kits are nowadays in the market, consequently this contract has been signed with the aim to supply UNISENSOR S.A. with immunoreagents for their[...]

19 Dec

NANBIOSIS in three projects funded by CaixaIMPULSE.
NANBIOSIS in three projects funded by CaixaIMPULSE

La Caixa has just presented the twenty projects selected by CaixaImpulse in a call with more than 70 proposals from different research centres, hospitals and universities. CaixaImpulse is an initiative unique in Spain that aims to boost research in biotechnology and life sciences to develop projects that generate value in society. Among the selected projects there are two coordinated by NANBIOSIS researchers: Pseudomonas aeruginosa diagnosis led by Miriam Corredor Sánchez (Unit 2 of NANBIOSIS) and ISCHEMSURG, led by Monica Mir (Unit 7 of NANBIOSIS). In addition, the project ExoLiver, in which participates Rosa Villa and the research group coordinating Unit 8[...]

06 Dec

Unit 2 of NANBIOSIS participates in the FoodSmartPhone project of Marie Sklodowska Curie.
Unit 2 of NANBIOSIS participates in the FoodSmartPhone project of Marie Sklodowska Curie.

The Nb4D group of CSIC, coordinator of Unit 2 of NANBIOSIS led by Pilar Marco has joined the consortium for the FoodSmartPhone project funded by the Marie Sklodowska Curie Research Program. The Marie Sklodowska-Curie Actions (MSCA) support training in research and professional development aimed at innovation skills. The objective of the project is to develop a smartphone-based diagnostic and detection tool for simplified quick pre-selection of food quality and safety parameters. This is because food safety and quality tests are currently inefficient, expensive and ineffective and can not guarantee food safety for all consumers or prevent possible incidents of food[...]

20 Apr

Nanbiosis_U2_Nanobiotechnology for Diagnostics Group awarded with TECNIO grant to encourage Technology Transfer
Nanobiotechnology for Diagnostics Group awarded with TECNIO grant to encourage Technology Transfer

The Nanobiotechnology for Diagnostics Group (Nb4D), the host group for the Custom Antibody Service of Unit 2 of NANBIOSIS was recently awarded with grant that ACCIÓ provides to groups who are seeking to be accredited with the TECNIO seal. The aim is to help define and boost their action plans for technology transfer. These grants are destined at financing for 3 years the integration of these groups in the UAB-CEI sphere of the TECNIO network. The group will use this finance for activities related to the execution during the first year of a growth plan to increase their technology transfer[...]

U2-Custom Antibody Service (CAbS)
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U3. Synthesis of Peptides Unit

Ciber bbn

U3. Synthesis of Peptides Unit

  • Scientific Director: Prof. Fernando Albericio albericio@irbbarcelona.org
  • Scientific Coordinator: Dr. Miriam Royo mroyo@pcb.ub.cat
  • Entities: Parc Cientific de Barcelona (PCB) & Universidad de Barcelona (UB)
  • Address: Baldiri Reixac 10, 08028, Barcelona, Spain
  • Phone: +34 934 037 122
  • Web:PCB – UB
PCB
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Description

This Unit is coordinated by the Peptides and Nanoparticles Group at the Parc Científic de Barcelona (PCB) and has the equipment necessary to provide services of synthesis of peptides at different scales (mg to g), purification, characterization, and post-synthesis modification, such as, conjugation to proteins and fluorescent. It counts with laboratories for synthesis and other to carry out modification of the peptides during and post synthesis labels. Also counts with a laboratory equipped with several preparative and analytical chromatography systems for purification and characterization. This facility benefits from the wide experience of Dr. Fernando Albericio in the design and synthesis of peptides with specific biological activity and the introduction of modifications necessary for these peptides to be bound to therapeutic nanoconjugates and other molecules, either to take advantage of the pharmacological activity of the peptide itself or to facilitate the introduction of nanoconjugates or other molecules into the cells in order to reach the therapeutic target. Its location allows the access to the rest of services of the PCB with an occupy area of 5.000m2.

News U3

28 Jun

“a-Galactosidase A Loaded Nanoliposomes with Enhanced Enzymatic Activity and Intracellular Penetration”
“a-Galactosidase A Loaded Nanoliposomes with Enhanced Enzymatic Activity and Intracellular Penetration”

Unit 1 of NANBIOSIS, Protein Production Platform (PPP), Unit 3, Synthesis of Peptides, Unit 20, In Vivo Experimental Platform and Unit 6, Biomaterial Processing and Nanostructuring Unit, have jointly developed the research conducted in relation with a CO2-based methodology for the one-step production of protein-nanoliposome conjugates as bio-active nanomaterials with therapeutic interest. The results have been published in Advanced Healthcare Materials: http://www.ncbi.nlm.nih.gov/pubmed/26890358 “a-Galactosidase A Loaded Nanoliposomes with Enhanced Enzymatic Activity and Intracellular Penetration I. Cabrera, I. Abasolo, J. L. Corchero, E. Elizondo,  P. Rivera, E. Moreno, J. Faraudo, S. Sala, D. Bueno, E. González-Mira, M. Rivas, M. Melgarejo, D. Pulido,[...]

U3-Synthesis of Peptides Unit
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U4. Biodeposition and Biodetection Unit

Ciber bbn

U4. Biodeposition and Biodetection Unit

  • Scientific Director: Prof. Laura Lechuga laura.lechuga@icn2.cat
  • Scientific Coordinator: Dr. MªCarmen Estevez mcarmen.estevez@icn2.cat
  • Entities: Research Center on Nanoscience and Nanotechnology CIN2 & CSIC
  • Address: Campus Bellaterra, 08193 Cerdanyola del Vallés, Barcelona, Spain
  • Service Contact mail: nanob2a@icn2.cat
  • Phone: +34 937 374 617
  • Web: CIN2
CIN2_U4 CSIC
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Description

Located at the Research Center onNanoscience and Nanotechnology(CIN2), CSIC, at the campus of Universidad Autónoma de Barcelona (UAB) the Biodeposition Unit consists of a versatile and automated molecular printer that can dispense minute volumes of solutions (from atto to femtoliter volumes) on a wide variety of surfaces to create user-defined patterns of spots or lines with high spatial resolution. The system is suitable for the functionalization of biosensors with any kind of biomolecule, nanoparticles, graphene or other nanostructures,for surface patterning for cell culture, or for the preparation of micro/nanoarrays, among other applications. The laboratory also harbors a Surface Plasmon Resonance (SPR) service. The Unit is coordinated by Prof. LauraM. Lechuga, group leader from the Nanobiosensorsand Bioanalytical Applications Group (nanoB2A) at CIN2-CSIC.

News U4

22 Jun

New methodology for cancer screening paves the way for more targeted treatment options

Laura Lechuga, Scientific Director of Unit 4 of NANBIOSIS, is co-author of a new paper published in Scientific Reports. According to Cesar Huertas, co-author of the article, the Gene expression is the process by which information from a gene is converted into a functional product, such as a protein. It is controlled by a complex regulatory network wherein diverse cellular mechanisms enable the cell to respond to its ever-changing environment. One such mechanism is alternative splicing (AS) of mRNA precursors, a particularly flexible regulatory control point where diverse protein isoforms of differing, even opposing, functions are generated. However, these regulation[...]

04 May

Laura Lechuga in the fight against cancer

Dr. Laura Lechuga, Scientific Director of Unit 4 of NANBIOSIS is highlighted in Cinco Días. Elpais Economía  for its fight against cancer and, in particular, for the device developed with its team to detect the disease in matter of minutes “Have you ever wondered why glucose meters are so smart that they just measure sugar? The answer is that they carry specific proteins that only interact with sugar, “explains the doctor.  The same idea lies in the device designed by Laura Lechuga and her research group “With a minimum sample of the patient, it is possible to detect the presenc[...]

06 Feb

A multiplexing nanophotonic biosensor for cancer diagnosis
A multiplexing nanophotonic biosensor for cancer diagnosis

The group Biosensors and bio-analytic applications, led by Laura Lechuga,​ coordinator of Unit 4 of NANBIOSIS, has developed a methodology through a nano-photonic sensor (known as bimodal waveguide interferometer) capable of quantifying, directly and mark-free, the different messenger RNA isoforms generated by alternative splicing. The expertise in biodeposition and biodetection systems of Lechuga’s group has permitted a thorough analytical study and optimization of the methodology, achieving not only total selectivity, but also excellent levels of sensitivity and reproducibility, and obtaining the lowest levels of detection in direct detection of messenger RNA isoforms generated by this mechanism without the need for PCR amplification (polymerase chain reaction). T[...]

19 Dec

Laura Lechuga received the Physics, Innovation and Technology Prize
Laura Lechuga received the Physics, Innovation and Technology Prize

On December 14th, the Physics Awards ceremony was held at the BBVA Foundation’s headquarters, organized by the Spanish Royal Society of Physics (RSEF) and the BBVA Foundation, that each year select the most relevant achievements of Spanish physics. On this occasion the achievements of nanoscience have been recognized and it has been highlighted the power of physics to expand the territory of the known universe and the role of scientists as a core element of social progress. Professor Laura Lechuga, Scientific Director of Unit 4 of NANBIOSIS received the Physics, Innovation and Technology Prize was the only woman awarded. Lechuga[...]

24 Oct

Physics Prize of the Royal Spanish Society of Physics (RSEF) to Laura Lechuga
Physics Prize of the Royal Spanish Society of Physics (RSEF) to Laura Lechuga

Laura M. Lechuga, Scientific Director of the Unit 4 of NANBIOSIS has won the Physics Prize of the Royal Spanish Society of Physics (RSEF) -Foundation BBVA 2016 in the form of Physics, Innovation and Technology, endowed with 8,000 euros. Physics Awards recognize this year to researchers who have revealed the potential of the “nanoworld” to create new materials and fight disease. The jury highlighted her “excellent track record as a scientist, technologist and innovative” in the field of biosensor devices. “Prof. Lechuga – emphasizes the jury- has managed to walk the path from idea to product with great success. Her[...]

03 Oct

Laura Lechuga, Scientific Director of the Unit 4 of NANBIOSIS in the Spanish Selection of Science.
Laura Lechuga, Scientific Director of the Unit 4 of NANBIOSIS in the Spanish Selection of Science.

The selection of the best Spanish researchers in 2016 is an initiative of the magazine QUO to disseminate the work of scientists. The jury formed by eight eminent scientists (Amils, Ainhoa Goni, Emilio Lora-Tamayo, Vladimir de Semir, Fernando Peláez, Antonio Calvo, Pilar López and Manuel Toharia, has chosen the twelve scientists of international prestige who will form the Spanish National Science 2016: – Pedro Cavadas, plastic surgeon specializing in transplants – Mariano Barbacid, a biochemist at the National Cancer Research Centre (CNIO) – Susana Marcos, director of the Institute of Optics ‘Daza Valdes’ CSIC – Alberto Ruiz Jimeno, founder of[...]

26 Aug

Nanotechnology bio applications in the clinical diagnosis II biosensor BiMW for the early diagnosis of bacterial infections in cirrhotic patients
Nanotechnology bio applications in the clinical diagnosis II: biosensor BiMW for the early diagnosis of bacterial infections in cirrhotic patients

The peritonitis bacterial spontaneous is an infection of the fluid ascetic that has high incidence in cirrhotic patients and is associated with a high mortality. In such situation, a diagnosis early is essential for the survival of the patient. However, the identification of the causative pathogen of infection is based currently on cultivation methods that are slow and laborious. A research coordinated by the Group of Nanobiosensors and Bioanalytical applications of the CIBER-BBN, which coordinates Unit 4 of NANBIOSIS led by Laura Lechuga, has presented the development of a biosensor interferometric based in guides bimodal (BiMW), capable of identify and[...]

25 Aug

Nanotechnology bio applications in the clinical diagnosis nanoplasmodic biosensor device for the early diagnosis of colorectal cancer
Nanotechnology bio applications in the clinical diagnosis: nanoplasmodic biosensor device for the early diagnosis of colorectal cancer

The group of Nano-biosensors and Bioanalytical applications of CIBER-BBN which coordinates Unit 4 of NANBIOSIS directed by Dr. Laura Lechuga, has led the final preparations of a technology with potential application in the early diagnosis of colorectal cancer, based in the detection of specific biomarkers in blood. The nanoplasmonic biosensor based on LSPR (Localized Surface Plasmon Resonance) is generated on structured surfaces of nano discs of gold, that allow monitoring of biological interactions in real time and without the need of markers. The nanoplasmonic biosensor allows analysis of human samples in a few minutes, without requiring reagents or additional instrumentation.[...]

U4-Biodeposition and Biodetection Unit
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Nanbiosis