The mAb development contains the following phases:
- Immunisation of mice, titre determination in the antiserum.
- Cultivation of the myeloma cell line, cell fusion, selection in HAT medium and screening.
- Cloning , screening and cultivation of positive cultures. Further cloning and obtaining stable clones.
- Expansion and Cryo conservation of the selected clones. Delivery of cell culture supernatants and frozen hybridoma cells.