U6-E19. Dynamic Light Scattering equipment

U6-E19.Dynamic LightScattering equipment

(Zetasizer ULTRA)

The Zetasizer ULTRA is used to measure the particle size of dispersed systems from sub-nanometer to several micrometers in diameter, using the technique of Dynamic Light Scattering (DLS). Zetasizer systems are also used to analyze particle mobility and charge (Zeta potential) using the technique of Electrophoretic Light Scattering (ELS), and the molecular weight of particles in solution using Static Light Scattering (SLS).

Technical specifications:

Particle size and molecular size

• Measurement principle: Non-Invasive Back Scatter (NIBS) Dynamic Light Scattering. Dynamic Light Scattering (90° and 13°) Multi-Angle Dynamic Light Scattering (MADLS)
• Measurement angle: 173º, 13º, 90º
• Size ranges: from 0.3 nm to 10 µm.
• Minimum volume: 3 µL
• Minimum sample concentration:
  • Blue Label: 0.2 mg/mL 15 kDa Protein
  • Red Label: 0.1 mg/mL 15 kDa Protein
• MADLS: 0.1 mg/ml 15 kDa Protein
• MADLS Particle Concentration Enabled
  • Blue Label: No
  • Red Label: Yes
• Maximum sample concentration: 40% w/v

Zeta potential

• Measurement principle: Mixed-Mode Measurement phase analysis light scattering (M3-PALS)
• Concentration range. Minimum sample concentration: 
  • Blue Label: 10 mg/mL to 40% w/v
  • Red Label: 1 mg/mL to 40% w/v
• Sample conductivity range: Maximum 260 mS/cm
• Size ranges: 3.8 nm to 100 µm
• Minimum volume: 20 µL

Compatible cells

A range of disposable and reusable cells are available to optimize the measurement in terms of sample volume and concentration, including the new low volume disposable sizing cell kit, which offers the dual benefits of size measurements in as little as 3 µL, and extension to the upper size range of DLS measurements, due to the suppression of convection currents.

Applications: The Zetasizer Ultra is suitable for a wide range of applications, including colloidal Silica, titanium dioxide, ceramics, proteins, carbon dots (C-dots), and adeno-associated virus (AAV).