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Nanoparticles as Contrast Agents for MRI and OCT

Compressed fluid-based technology platform for biomaterials processing

Scientific leaders of the project: Prof. Nora Ventosa, Prof. Jaume Veciana and Prof. Jesús Santamaria.
Coordinator of the project: Dr Gemma Martinez and Dr. Nathaly Segovia

Industrial problem/gap covered

The conventional approaches used for the production of particulate molecular biomaterials usually follow easy preparation methods at the laboratory scale, but they fail when scaling-up to industrial level.

Description

NANBIOSIS approach offers novel synthetic strategies and experimental setup for the advanced preparation of a wide range of nanoparticles for MRIcontrast agents.

In the development of this biomedical solution, the following services are involved:

Au Magnetic /SiO2nanoshellwith a spions located inside

Unit Type of NP Specifications of the NP (Size, composition, etc) Applications & reference
U9 Au Magnetic /SiO2nanoshellwith a spions located inside  

 

Magnetic nanoparticles in the core, covered by a shell of SiO2 with an external layer of gold, and average diameter of 100 nm

 

Application: Photothermal therapy and MRI signaling (theranosticnanoparticles)

 

Ref:

– Nanoscale. 2014 Aug 7;6(15):9230-40.

Hollow Au nanoshell with a spion located inside

Unit Type of NP Specifications of the NP (Size, composition, etc) Applications & reference
U9 Hollow Au nanoshell with a spion located inside  

 

Magnetic Gold-based nanoparticles with magnetic and optical properties. Spherical shape and average diameter of 150 nm

 

Application: Photothermal therapy and MRI signaling (theranostic nanoparticles)

 

Ref:

– Nanoscale. 2014 Aug 7;6(15):9230-40.

Au nanorods capped with lysine

Unit Type of NP Specifications of the NP (Size, composition, etc) Applications & reference
U9 Au nanorods capped with lysine  

 

Gold-based nanorods

 

Application: Contrast agents for optical coherence tomography

 

Ref:

 

– ChemCommun (Camb). 2012 Jul 7; 48(53): 6654–6656

Magnetic nanoparticles

Unit Type of NP Specifications of the NP (Size, composition, etc) Applications & reference
U9 Magnetic nanoparticles  

 

Iron-based nanoparticles with spherical shape and average diameters ranged between 6 -13 nm

 

Application: MRI diagnosis (T2 contrast agents)

 

Ref:

 

J Nanopart Res (2014) 16:2292

Polymeric nanoparticles with an Au nanoparticles inside

Unit Type of NP Specifications of the NP (Size, composition, etc) Applications & reference
U9 Polymeric nanoparticles with an Au nanoparticles inside  

 

Application: Drug delivery and contrast agents (theranostics)

 

Ref:

 

Nanoscale, 2016,8, 6495-6506

Radical dendrimers as CA for MRI

Unit Type of NP Specifications of the NP (Size, composition, etc) Applications & reference
U6 Schematic representation of a Radical dendrimer  

 

Application: Contrast agents

 

Ref:

Organic Letters, 2013. 15(14): p. 3490-3493.

Macromolecules, 2014. 47(22): p. 7717-7724.

ChemPhysChem, 2015. 16(15): p. 3302-3307.

 

It gathers several laboratories, perfectly equipped, to perform the mission of this facility: the development, characterization, and large-scale production of molecular biomaterials of therapeutic or biomedical interest, with controlled micro-, nano- and supramolecular structure. One example of Key-Enabling-Technology (KET) available in this unit is a simple one-step methodology, DELOS-SUSP, based on the use of compressed fluids (CF), such as CO2, to prepare particulate materials with precise and reproducible structural characteristics at micro-, nano- and supramolecular levels (size, shape, internal structural gradients, supra­molecular organization and crystalline purity).This example shows one of the singularities of this unit is that counts with CF–based plants at different scales, from mL to L, which allow process development by QbD and process scale-up.

This unit offers total characterization of radical dendrimers (structural characterization, paramagnetic properties, in vitro CA imaging properties)

– Structural characterization: NMR, UV-vis, FTIR
– Paramagnetic properties: EPR
– Relaxivity properties (MRI in vitro)

 

This unit offers novel synthetic strategies and experimental setup for the advanced preparation of a wide range of nanoparticles, including inorganic and soft nanoparticles with biomedical application.

The new methodologies include microreactors, laser pyrolysis reactor and electro spinning. Both laser pyrolysis and microreactors as belonging to the group of enabling technologies, which allow new goals in reproducibility and scale-up production of nanomaterials. As for the electro spinning, this is a new infrastructure that allows the preparation of nanowires and fibres, formed by different materials.

This unit offers total characterization of nanoparticles, nanstructures and/or colloidal suspension of nanoparticles:

– Composition (chemical analysis)
– Structure (UV-vis, NIR, XPS, FTIR, TEM, SEM)
– Suface (Gas adsorption)
– Specific properties of the nanoparticles, such as, magnetic and optical properties.

Additionally, the U9 offers study and capacity for controlled adsorption and drug delivery.