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U1. Protein Production Platform (PPP)

U1. Protein Production Platform (PPP)

U1-S05. Training courses in protein Production (On-site&Remote)

e.g. Course on “Strategies for optimization of recombinant protein production”)

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U1.E05. Tangential filtration Unit for purification

Tangential filtration Unit for purification.

U1.E05_TANGENTIAL FILTRATION UNIT FOR PURIFICATION_1
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U1.E04. FPLC ÄKTA Chromatography systems for purification

ÄKTA pure™ is a flexible and intuitive chromatography system for fast purification of proteins, peptides, and nucleic acids from microgram levels to tens of grams of target product.

ÄKTA pure™ chromatography system provides you with the following benefits:
• Modular system design with a large range of options to allow flexibility in purification of proteins and peptides
• Intuitive and flexible method creation, system control, and evaluation with UNICORN™ software
• Practical size, for easy placement on laboratory bench or in cold cabinet
• Reliable system with components and integrated
features based on the proven design of ÄKTA™ protein purification systems
• Predefined method settings for all our laboratory-scale chromatography columns

A double-door refrigerator is available to contain FPLC-AKTA purification equipment inside, refrigerated, to be able to carry out low-temperature purifications of proteins that are sensitive to room temperature.

It also has a probe for acquiring temperature data from the refrigerator, to be able to track at all times the status of the samples that are stored inside the services during the protein production and purification service process and after

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ÄKTA Purifier equipment for protein purification
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U1.E03. Incubator shaker with cooling unit for insect cells suspension cultures

Incubator shaker with cooling unit for insect cells suspension cultures.

U1.E03_INCUBATOR SHAKER WITH COOLING UNIT FOR INSECT CELL SUSPENSION
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U1.E02. 10 liter WAVE bioreactor for insect cells

10 liter WAVE bioreactor for insect cells.

U1.E02_10 LITER WAVE BIOREACTOR FOR INSECT CELLS
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U1.E01. 2 and 7 liter bioreactors for bio production

2 and 7 liter bioreactors for bio production

U1.E01_2 AND 7 LITER BIOREACTORS FOR BIO PRODUCTION_1
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U1-S06. Criopreservation (On-site&Remote)

Criopreservation of Proteins, Plasmids, etc

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U1-S04. Consultancy in protein production (On-site&Remote)

In all matters dealing with protein production, expresion systems, etc, according to the final application of the product.

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U1-S03: Proteins purification (On-site&Remote) OUTSTANDING

  • Identification of the most appropriate strategy for purification, given the physical and chemical properties, opting either for purification modules or, in the case of fusion proteins, elements which enable purification by affinity.
  • Purification (downstream processing) using FPLC chromatography and/or tangential filtration.
  • Quality control of the purified protein ( molecular weight and purity by SDS-PAGE, biological activity, conformation by FTIR and DLS.
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U1-S02. Bioproduction of proteins (On-site&Remote) OUTSTANDING

  • Production of the plasmid DNA that contains the gene of the relevant protein to be incorporated into the producing cells by transformation/transfection.
  • Selection of the optimal protein production conditions using small and medium-scale expression; confirmation of the expression using Westernblot and SDS-PAGE analysis and quantification by densitometry.
  • Measurements of the solubility/aggregation of the protein under study.
  • Scale-up of the upstream processing in bioreactors or incubator shaker according to requirements.”
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