29 Apr19:57pm
Nanbiosis
- Identification of the most appropriate strategy for purification, given the physical and chemical properties, opting either for purification modules or, in the case of fusion proteins, elements which enable purification by affinity.
- Purification (downstream processing) using FPLC chromatography and/or tangential filtration.
- Quality control of the purified protein ( molecular weight and purity by SDS-PAGE, biological activity, conformation by FTIR and DLS.