U3. Synthesis of Peptides Unit

U3-S02. Post synthesis peptide modification (On-site&Remote) OUTSTANDING

Post synthesis peptide modification (On-site&Remote) OUTSTANDING

Post-synthesis peptide modification such as:
– Cyclization through a disulphide bridge, amide, ester, thiosester, thioether, or various staple bonds.
– Biotinylation and/or incorporation of various imaging probes at the N-terminus, C-terminus or on some amino acid side chains (Lys, Cys…).
– Convenient peptide derivatisation for further conjugation by introducing a maleimide, succinimidyl, alkyne or azide-containing spacers.
– Peptide conjugation to polymers, proteins, probes and small molecules.
Multivalent peptide presentation molecules.

Customer benefits

• Extensive experience in the synthesis of cyclic peptides (disulfide bridge, lactam, lactone, thioester, thioether).
• Extensive experience in the synthesis of stapled peptides
• Development of several peptide cyclization methods.
• Extensive experience in the derivatisation of peptides for conjugation and attachment to various types of molecules (nanoparticles, polymers, imaging probes, small molecules).
• Development of various strategies for the introduction of post-synthesis modifications.
• Development of conjugation methodology.
• Development of methodology for the generation of multivalent peptide presentation molecules.

Target customer

• Research groups (drug delivery, molecular biology, pharmacology, nanotechnology, biotechnology)
• Companies (biotech and pharma companies).

References

• Direct Quantitative Immunochemical Analysis of Autoinducer Peptide IV for Diagnosing and Stratifying Staphylococcus aureus Infections. Montagut, Enrique-J.; Acosta, Gerardo; Albericio, Fernando; Royo, Miriam; Godoy-Tena, Gerard; Lacoma, Alicia ; Prat, Cristina ; Salvador, Juan-Pablo; Marco, Maria-Pilar. ACS Infectious Diseases (2022), 8, 645-656.
• Hierarchical Quatsome-RGD Nanoarchitectonic Surfaces for Enhanced Integrin-Mediated Cell Adhesion. Martinez-Miguel, Marc; Castellote-Borrell, Miquel; Kober, Mariana; Kyvik, Adriana R. ; Tomsen-Melero, Judit ; Vargas-Nadal, Guillem; Munoz, Jose; Pulido, Daniel ; Cristobal-Lecina, Edgar; Passemard, Solene; oyo, Miriam ; Mas-Torrent, Marta ; Veciana, Jaume ; Giannotti, Marina I. ; Guasch, Judith ; Ventosa, Nora ; Ratera, Imma. ACS Applied Materials & Interfaces (2022), 14, 48179-48193.
• Engineering a Nanostructured Nucleolin-Binding Peptide for Intracellular Drug Delivery in Triple-Negative Breast Cancer Stem Cells. Pesarrodona, Mireia; Sanchez-Garcia, Laura; Seras-Franzoso, Joaquin; Sanchez-Chardi, Alejandro; Balta-Foix, Ricardo; Camara-Sanchez, Patricia; Gener, Petra; Jara, Jose Juan; Pulido, Daniel ; Serna, Naroa; Schwartz, Simo; Royo, Miriam ; Villaverde, Antonio; Abasolo, Ibane ; Vazquez, Esther. ACS Applied Materials & Interfaces (2020), 12, 5381-5388.
• Synthesis of Stable Cholesteryl-Polyethylene Glycol-Peptide Conjugates with Non-Disperse Polyethylene Glycol Lengths. Cristobal-Lecina, Edgar; Pulido, Daniel; Martin-Malpartida, Pau; Macias, Maria J.; Albericio, Fernando; Royo, Miriam. ACS Omega (2020), 5, 5508-5519.
• Highly Versatile Polyelectrolyte Complexes for Improving the Enzyme Replacement Therapy of Lysosomal Storage Disorders. Giannotti, Marina I.; Abasolo, Ibane; Oliva, Mireia; Andrade, Fernanda; Garcia-Aranda, Natalia; Melgarejo, Marta; Pulido, Daniel; Corchero, Jose L.; Fernandez, Yolanda; Villaverde, Antonio; Royo, Miriam; Garcia-Parajo, Maria F.; Sanz, Fausto; Schwartz, Simo. ACS Applied Materials & Interfaces (2016), 8(39), 25741-25752.
• Gated mesoporous silica nanoparticles using a double-role circular peptide for the controlled and target-preferential release of doxorubicin in CXCR4-expressing lymphoma cells. de la Torre, Cristina; Casanova, Isolda; Acosta, Gerardo; Coll, Carmen; Moreno, Maria Jose; Albericio, Fernando; Aznar, Elena; Mangues, Ramon; Royo, Miriam; Sancenon, Felix; Martinez-Manez, Ramon. Advanced Functional Materials (2015), 25, 687-695.
• Multivalent dendrimers presenting spatially controlled clusters of binding epitopes in thermoresponsive hyaluronan hydrogels. Seelbach, Ryan J.; Fransen, Peter; Peroglio, Marianna; Pulido, Daniel; Lopez-Chicon, Patricia; Duttenhoefer, Fabian ; Sauerbier, Sebastian; Freiman, Thomas; Niemeyer, Philipp; Semino, Carlos; Albericio, Fernando; Alini, Mauro; Royo, Miriam; Mata, Alvaro; Eglin, David. Acta Biomaterialia (2014), 10, 4340-4350
• Triazene as a powerful tool for solid-phase derivatization of phenylalanine containing peptides: Zygosporamide analogues as a proof of concept. Torres-Garcia, Carolina; Pulido, Daniel; Albericio, Fernando; Royo, Miriam; Nicolas, Ernesto. Journal of Organic Chemistry (2014), 79(23), 11409-11415.