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Preclinical validation tissue, biomaterialls and surfaces characterization -Services

Preclinical validation tissue, biomaterialls and surfaces characterization -Services

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U30-S03. Protein identification by mass spectrometry (MS) or MS/MS

Protein identification by mass spectrometry (MS) or MS/MS

Identification of proteins in biological samples, either by intact protein analysis (MALDI-TOF) or protein digestion followed by peptide mass fingerprinting (PMF, MS) or peptide fragmentation (MS/MS).

Customer benefits

Identification of proteins by mass spectrometry allows the characterization of biological samples, analysis of protein interactions (e.g. pull-down assays), peptide sequencing, etc. Identifications are performed with the highest specificity.

Target customer

Organizations and research groups interested in small or large-scale protein analysis.

Additional information

E03 MALDI-TOF/TOF System

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U30-S02. Determination of molecular mass of peptides, proteins and other biomolecules

Determination of molecular mass of peptides, proteins and other biomolecules

Analysis of intact proteins, peptides and other biomolecules such as oligonucleotides using mass spectrometry (MALDI-TOF or ESI-Q-TOF) for the determination of their exact molecular mass.

Customer benefits

This service is useful to characterize a variety of biomolecules by the determination of their molecular mass with an extreme high precision. This is an essential step in many biotechnological strategies involving protein synthesis and production.

Target customer

Organizations or research groups aiming to characterize specific proteins, peptides or other biomolecules by the determination of their molecular mass.

U30 S02 Determination of molecular mass of peptides, proteins and other biomolecules
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U30-S01. Sample preparation for proteomic analysis

Sample preparation for proteomic analysis

This service includes all necessary steps to prepare samples for different proteomic analyses: protein extraction from tissues, cells or fluids, precipitation, cleaning, desalting, quantification and digestion (in gel or in solution) with proteolytic enzimes (trypsin, chymotrypsin, lys-C…), to obtain the correspondent peptides.

Customer benefits

Biological samples are processed and prepared following standardized protocols by personnel highly specialized in techniques for protein analysis.
The service allows organizations without prior experience in protein or proteomic analysis to avoid performing any experiment, only needing to provide the biological specimen to the Unit.

Target customer

Organizations or research groups interested in performing protein or proteomic analyses in a wide range of biological samples: cells, tissues, biofluids, protein extracts, etc.

U30 S01 Sample preparation for proteomic analysis
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U19-S03. Clinical Analysis Laboratory

Clinical Analysis Laboratory

The clinical analysis laboratory that performs a wide variety of analysis: (biochemistry, hematology, coagulation, urine analysis, blood gas analysis, different biomarkers, hormones, allergens) in various animal species (rat, mouse, rabbit, pig, sheep, dog, and cat). Specific methodologies are used in each animal species, having all techniques validated according to their physiological and pathological identity. It also has a formulation laboratory equipped to formulate, reconstitute and guarantee the correct storage of the test products used in these studies.

Customer benefits

The laboratories are certified with ISO-9001 and Good Laboratory Practices (GLP), strict quality standards that allow the production of high-precision results.
Also, the studies can be performed under regulatory requirements too, since the performing institution is Certified for Good Laboratory Practices.
Therefore, studies can be carried out in compliance with the strict guidelines of regulatory agencies, ensuring the reliability and traceability of all results and tests carried out in their different services.

Target customer

The services offered in this unit may be of interest to different companies and laboratories that work within the pharmaceutical industry. Companies whose objective is to test possible candidates for molecules, drugs or medical devices.
With this service, companies and laboratories ensure that they report reliable and reproducible results in all their studies, guaranteeing the traceability and viability of analyzes obtained.

References

  1. J Bote, et al. Novel ex-vivo database of a murine model of colorectal cancer using optical coherence tomography. Surg Endosc (2022) 36:S325–S674
  2. V Lucas-Cava, et al. Prostatic artery occlusion: Initial findings on pathophysiological response in a canine prostate model. Translational Andrology and Urology. Transl Androl Urol 2022.
  3. Soria F, et al. Heparin coating in biodegradable ureteral stents does not decrease bacterial colonization-assessment in ureteral stricture endourological treatment in animal model. Transl Androl Urol. 2021 Apr;10(4):1700-1710.
  4. Baez-Díaz C, et al. Microencapsulated Insulin-Like Growth Factor-1 therapy improves cardiac function and reduces fibrosis in a porcine acute myocardial infarction model. Sci Rep. 2020 Apr 28;10(1):7166.
  5. Moreno-Lobato, B, et al. Use of nanomedicine in preclinical wound healing studies. FarmaJournal; Salamanca T 4, N.º 1, (Feb 2019): 178.
  6. Picado Román, N, et al. Drugs most used in experimental animals in a Research Center. FarmaJournal; Salamanca T 4, N.º 1, (Feb 2019): 236.
  7. Moreno-Lobato B, et al. Analytical Validation Study of Hematological Parameters under Good Laboratory Practice Regulations in Different Laboratory Animal Species. Thromb Haemost Res. 2019; 3(1): 1018.
  8. Ballestín A, et al. A Pre-clinical Rat Model for the Study of Ischemia reperfusion Injury in Reconstructive Microsurgery. J Vis Exp. 2019 Nov 8;(153).
  9. R Blázquez , et al. Altered hematological, biochemical and immunological parameters in a porcine myocardial infarction model: predictive biomarkers for the severity of myocardial infarction. Veterinary Immunology and Immunopathology 205 (2018) 49–57.
  10. Vela FJ, et al. Evaluation of antigen-induced synovitis in a porcine model: Immunological, arthroscopic and kinetic studies. BMC Vet Res. 2017. Apr 7;13(1):93.
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U14-S06. Lymphocyte proliferation assay (On-site&Remote) OUTSTANDING

Lymphocyte proliferation assay

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U14-S05. Determination of cytokine concentration by flow cytometry (On-site&Remote) OUTSTANDING

Determination of cytokine concentration by flow cytometry

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U14-S04. Cytokine quantification assays (On-site&Remote) OUTSTANDING

Cytokine quantification assays

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U14-S03. Biomecanic evaluation of plantar pressure in large animal models (On-site) OUTSTANDING

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U13-S08. Atomic Force Microscopy indentation test

Atomic Force Microscopy indentation test

Atomic Force Microscopy can be used to measure the forces between the probe and the sample as a function of their mutual separation. This can be applied to perform force spectroscopy.

Customer benefits

Low forces on cells (nano-scale).

Target customer

Public and private research groups focused on mechanobiology

Additional information

Cantilever + cells
Force curve
Capsules
AFM Software
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U19-S01. Preclinical studies

Preclinical studies

This service is responsible for conducting regulatory preclinical studies for the pharmaceutical industry and interested companies. Among the studies that can be carried out are: in vivo toxicity studies, local and systemic tolerance and efficacy studies. Pharmacokinetic studies, dosage studies of test products and biocompatibility studies of new drugs and medical devices are also performed.

Customer benefits

These studies are carried out under strict quality regulations, certified with ISO-9001 and Good Laboratory Practices (GLP), quality standards that allow the production of high-precision results.
Therefore, preclinical studies can be carried out in compliance with the strict guidelines of regulatory agencies, ensuring the reliability and traceability of all results and tests carried out in their different services.

Target customer

The services offered may be of interest to different companies and laboratories that work within the pharmaceutical industry. Companies whose objective is to test possible candidates for molecules, drugs or medical devices.

References

  1. J Bote, et al. Novel ex-vivo database of a murine model of colorectal cancer using optical coherence tomography. Surg Endosc (2022) 36:S325–S674
  2. V Lucas-Cava, et al. Prostatic artery occlusion: Initial findings on pathophysiological response in a canine prostate model. Translational Andrology and Urology. Transl Androl Urol 2022.
  3. Soria F, et al. Heparin coating in biodegradable ureteral stents does not decrease bacterial colonization-assessment in ureteral stricture endourological treatment in animal model. Transl Androl Urol. 2021 Apr;10(4):1700-1710.
  4. Baez-Díaz C, et al. Microencapsulated Insulin-Like Growth Factor-1 therapy improves cardiac function and reduces fibrosis in a porcine acute myocardial infarction model. Sci Rep. 2020 Apr 28;10(1):7166.
  5. Moreno-Lobato, B, et al. Use of nanomedicine in preclinical wound healing studies. FarmaJournal; Salamanca T 4, N.º 1, (Feb 2019): 178.
  6. Picado Román, N, et al. Drugs most used in experimental animals in a Research Center. FarmaJournal; Salamanca T 4, N.º 1, (Feb 2019): 236.
  7. Moreno-Lobato B, et al. Analytical Validation Study of Hematological Parameters under Good Laboratory Practice Regulations in Different Laboratory Animal Species. Thromb Haemost Res. 2019; 3(1): 1018.
  8. Ballestín A, et al. A Pre-clinical Rat Model for the Study of Ischemia reperfusion Injury in Reconstructive Microsurgery. J Vis Exp. 2019 Nov 8;(153).
  9. R Blázquez , et al. Altered hematological, biochemical and immunological parameters in a porcine myocardial infarction model: predictive biomarkers for the severity of myocardial infarction. Veterinary Immunology and Immunopathology 205 (2018) 49–57.
  10. Vela FJ, et al. Evaluation of antigen-induced synovitis in a porcine model: Immunological, arthroscopic and kinetic studies. BMC Vet Res. 2017. Apr 7;13(1):93.
• Read More