+34 620 10 75 37info@nanbiosis.com

Preclinical validation tissue, biomaterialls and surfaces characterization -Services

Preclinical validation tissue, biomaterialls and surfaces characterization -Services

U16-S09. Kelvin Probe Force Microscopy (KPFM) and Atomic Force Microscopy (AFM)

Electrical properties of solid surfaces using Kelvin Probe Force Microscopy (KPFM) and mechanical and topographical quantification using Atomic Force Microscopy (AFM)

The AFM Dimension Icon-PT has a microscopy method that allows to obtain the electrical properties of the surface of a material in air or liquid. In addition, the mechanical and viscoelastic properties of these surfaces can be determined and their morphology and topography can be studied qualitatively and quantitatively through images and 3D roughness parameters. The measuring range of this equipment reaches tenths of nanometres in height and hundreds of nanometres in spacing.

Customer benefits

The service will be provided by specialists in characterisation of materials in micro-nanoscopic dimensions.
The service works in accordance with ISO, ASTM, ASME and EUR standards. In addition, it can also provide detailed reports according to the needs of the study.

Target customer

The service can meet the needs of companies by providing support for their ISO, ASTM, ASME and EUR controls. In addition, it can also service research groups with detailed reports according to the needs of the study.

Additional information

Read More

U16-S08. Electrical characterisation of surfaces through the determination of zeta potential

Electrical characterisation of surfaces through the determination of zeta potential

This service allows the determination of the electrical interaction potential or zeta potential of large surfaces with different geometries and sizes such as flat solids, powders, fibres, foils or porous materials. It allows working with conductive and non-conductive materials. By determining the zeta potential, this service can determine the surface electrical properties of these materials. It also makes it possible to monitor the electrical changes that a surface may undergo during adsorption-desorption processes of different molecular species. The electrical effects of physical or chemical treatments applied to the surface of materials can be known. The removal of trace impurities present on the surface of a sample during a cleaning process can be monitored electrically.

Customer benefits

The service will be provided by specialists in materials characterisation and works in accordance with ISO, ASTM, ASME and EUR standards. In addition, it can also provide detailed reports according to the needs of the study.

Target customer

The service can meet the needs of companies by providing support for their ISO, ASTM, ASME and EUR controls. In addition, it can also service research groups with detailed reports according to the needs of the study.

Additional information

Read More

U16-S07. Evaluation of the adhesion capacities and formation of biolayers of microorganisms on materials. Evaluation of bactericidal and/or bacteriostatic responses of materials. OUTSTANDING

Description:

Evaluation of the adhesion of microorganisms to surfaces, including the evaluation of their viability. Adhesion can be studied under flow or static and at different contact times. Study of biolayers formed on materials after different growth times. Evaluation of bactericidal and/or bacteriostatic responses using viability kits or plate growth.


Applications:

Testing of materials with anti-infective objectives. Testing of compounds with antibacterial purpose.

Read More

U16-S06. Topographical characterisation service using optical profilometry

Topographical characterisation service using optical profilometry

This service makes use of a non-invasive optical method to determine the 3D texture of surfaces using spatial, volumetric and height parameters, from the millimetre to the nanometre range. Confocal profilometry can be used to obtain a complete surface characterisation with roughness parameters, as well as a dimensional characterisation of living microorganisms and their structures. In addition, this service has a high-resolution technique for the characterisation of surfaces with roughness in the nanometre range, such as interferometry, and a fast technique for surfaces with roughness of hundreds of microns, such as focus variation.

Customer benefits

The service will be provided by specialists in characterisation of materials in macroscopic dimensions and in living micro-organisms.
The service works in accordance with ISO, ASTM, ASME and EUR standards. In addition, it can also provide detailed reports according to the needs of the study.

Target customer

The service can meet the needs of companies by providing support for their ISO, ASTM, ASME and EUR controls. In addition, it can also service research groups with detailed reports according to the needs of the study.

Additional information

Read More

U16-S05. Contact angle goniometry characterisation service

Contact angle goniometry characterisation service

This service permits the determination of the surface tension of both solids and liquids under controlled conditions of temperature and humidity, including locally by means of deposited droplets of a volume of the order of 10-12 pL. The service offers the possibility to perform static, advancing and receding contact angle measurements between different liquids and a solid surface. Surface tension and liquid-liquid interfacial tension measurements can also be carried out using the pendant drop method. All these measurements can be carried out in a humidity and temperature controlled environment.

Customer benefits

The service will be provided by specialists in characterisation of materials in macroscopic dimensions or in colloidal systems.
The service works in accordance with ISO, ASTM, ASME and EUR standards. In addition, it can also provide detailed reports according to the needs of the study.

Target customer

The service can meet the needs of companies by providing support for their ISO, ASTM, ASME and EUR controls. In addition, it can also service research groups with detailed reports according to the needs of the study.

Additional information

Read More

U13-S09. Compression-shear test cellscale MicroTester G2

Compression-shear test cellscale MicroTester G2

Compression and shear test applied to a wide range of biomaterial and soft tissues. Force is measured using the difference between the tip and base displacement and the stiffness of the beam that connects them.
Mechanical and viscoelastic properties can be measured.

Customer benefits

  • Adaptable system to the material stiffness properties.
  • Well define testing protocols.
  • Tests are recorded in high definition.

Target customer

Public and private research groups focused on biomaterials and tissue mechanical characterization.

References

Nicolás Laita, Ricardo M. Rosales, Ming Wu, Piet Claus, Stefan Janssens, Miguel Ángel Martínez, Manuel Doblaré, Estefanía Peña, On modeling the in vivo ventricular passive mechanical behavior from in vitro experimental properties in porcine hearts, Computers & Structures, 292, 2024, 107241, ISSN 0045-7949, Doi: 10.1016/j.compstruc.2023.107241.

Additional information

Used in BravE project (https://projectbrave.eu/)

Read More

U30-S09. Data analysis of proteomics experiments

Data analysis of proteomics experiments

Use of dedicated software for the analysis and interpretation of the results obtained from the proteomic analyses. It includes search engines (MaxQuant, DIANN) for protein identification and quantification, software for statistical analysis (Perseus) and programs for integrated solutions (Peaks) that also enable the analysis of post-translational modifications (PTMs), sequence variants and mutations.

Customer benefits

This service provides comprehensive reports of the data obtained in the proteomic analyses, following the customer’s indications and the objective of each specific project. Upon request, data and reports can be uploaded to private or public repositories.

Target customer

Organizations and research groups focused in biomarker discovery, metaproteomics studies, analysis of specific post-translational modifications, etc.

U30 S09 Data analysis of proteomics experiments
Read More

U30-S08. Luminex or ELISA assay development and analysis

Luminex or ELISA assay development and analysis

Design and development of immunoassays employing antibody pairs for the detection and absolute quantification of target proteins. These can be set up on a traditional ELISA format, or coupled to magnetic beads (xMAP, Luminex) for multiplex detection. Optimization procedures, including cross-reactivity assays, are performed to standardize the assay protocol. Alternatively, this service may also use commercial ELISA or multiplex kits for the analysis of specific protein panels.

Customer benefits

This service provides quantitative data of proteins and small protein panels, including cytokines, chemokines or specific biomarker candidates, in biological samples. The assays are run with high sensitivity and throughput.

Target customer

Organizations and research groups that aim to quantify in an absolute way single proteins or small panels in large number of samples. Groups interested in cytokine or chemokine profiling.

U30-S08 : Luminex or ELISA assay development and analysis
Read More

U30-S07. Protein separation by one- or two-dimensional gel electrophoresis

Protein separation by one- or two-dimensional gel electrophoresis

Separation of complex protein mixtures on isoelectric focusing strips (according to their charge) and/or SDS-PAGE gels (according to their size), as a step for further protein or proteomic analysis.

Customer benefits

This service allows the fractionation of complex protein samples prior to proteomic analysis, performing gel-based quantitative proteomic studies by DIGE (differential in-gel electrophoresis), and evaluating protein profiles by SDS-PAGE followed by gel staining.

Target customer

Organizations and research groups interested in protein or proteomic analysis of biological samples.

U30-S07 Protein separation by one- or two-dimensional gel electrophoresis
Read More

U30-S06. Protein or peptide separation by liquid chromatography

Protein or peptide separation by liquid chromatography

Separation of complex protein or peptide mixtures using chromatography columns. The chromatographic services offered by the Unit include strong cation exchange (IEX), reversed phase (C18), gel filtration and immunoaffinity for the removal of the top 14 most abundant proteins in human plasma.

Customer benefits

Fractionation of complex protein or peptide samples prior to proteomic or protein analysis. Processing of serum, plasma and other biofluids for shotgun proteomics analysis through the depletion of their most abundant proteins.

Target customer

This strategy is employed to verify or validate biological hypotheses with the highest specificity, as it detects the protein sequence and is not dependent on antibodies.

U30 S06 Protein or peptide separation by liquid chromatography
Read More