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Preclinical validation tissue, biomaterialls and surfaces characterization -Services

Preclinical validation tissue, biomaterialls and surfaces characterization -Services

U16-S07. Evaluation of the adhesion capacities and formation of biolayers of microorganisms on materials. Evaluation of bactericidal and/or bacteriostatic responses of materials. OUTSTANDING

Description:

Evaluation of the adhesion of microorganisms to surfaces, including the evaluation of their viability. Adhesion can be studied under flow or static and at different contact times. Study of biolayers formed on materials after different growth times. Evaluation of bactericidal and/or bacteriostatic responses using viability kits or plate growth.


Applications:

Testing of materials with anti-infective objectives. Testing of compounds with antibacterial purpose.

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U16-S06. Topographic characterization service using optical profilometry OUTSTANDING

This service makes it possible to determine the surface tension of both solids and liquids under controlled temperature and humidity conditions, even locally by means of deposited drops with a volume of around 10-12 L. The service will be provided by specialists in the characterization of materials in dimensions macroscopic or in colloidal systems. The service can meet the needs of business R&D&i, providing support for their controls according to ISO, ASTM, ASME and EUR standards. In addition, it can also serve research groups with detailed reports according to the needs of the study.

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U16-S05. Characterization service by contact angle goniometry OUTSTANDING

This service makes it possible to determine the surface tension of both solids and liquids under controlled temperature and humidity conditions, even locally by means of deposited drops with a volume of around 10-12 L. The service will be provided by specialists in the characterization of materials in dimensions macroscopic or in colloidal systems. The service can meet the needs of business R&D&i, providing support for their controls according to ISO, ASTM, ASME and EUR standards. In addition, it can also serve research groups with detailed reports according to the needs of the study.

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U30-S09. Data analysis of proteomics experiments

Data analysis of proteomics experiments

Description: Use of dedicated software for the analysis and interpretation of the results obtained from the proteomic analyses. It includes search engines (MaxQuant, MSFragger) for protein identification and quantification, software for statistical analysis (Perseus) and programs for integrated solutions (Peaks) that also enable the analysis of post-translational modifications (PTMs), sequence variants and mutations.

Applications: Biomarker discovery, proteomic profiling, metaproteomics studies, PTM analysis, etc.

U30 S09 Data analysis of proteomics experiments
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U30-S08. Luminex or ELISA assay development and analysis

Luminex or ELISA assay development and analysis

Description: Design and development of immunoassays employing antibody pairs for the detection and absolute quantification of target proteins. These can be set up on a traditional ELISA format, or coupled to magnetic beads (xMAP, Luminex) for multiplex detection. Optimization procedures, including cross-reactivity assays, are performed to standardize the assay protocol. Alternatively, this service may also use commercial ELISA or multiplex kits for the analysis of specific protein panels.

Applications: Quantification of proteins and protein panels, including cytokines, chemokines or specific biomarker candidates, in biological samples.

U30-S08 : Luminex or ELISA assay development and analysis
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U30-S07. Protein separation by one- or two-dimensional gel electrophoresis

Protein separation by one- or two-dimensional gel electrophoresis

Description: Separation of complex protein mixtures on isoelectric focusing strips (according to their charge) and/or SDS-PAGE gels (according to their size), as a step for further protein or proteomic analysis.

Applications: Fractionation of complex protein samples prior to proteomic analysis, gel-based quantitative proteomic studies by DIGE (differential in-gel electrophoresis), evaluation of protein profiles.

U30-S07 Protein separation by one- or two-dimensional gel electrophoresis
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U30-S06. Protein or peptide separation by liquid chromatography

Protein or peptide separation by liquid chromatography

Description: Separation of complex protein or peptide mixtures using chromatography columns. The chromatographic services offered by the Unit include strong cation exchange (IEX), reversed phase (C18), gel filtration and immunoaffinity for the removal of the top 14 most abundant proteins in human plasma.

Applications: Fractionation of complex protein or peptide samples prior to proteomic analysis, preparation of serum, plasma and other biofluids for shotgun proteomics through the depletion of their most abundant proteins.

U30 S06 Protein or peptide separation by liquid chromatography
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U30-S05. Targeted proteomics analysis by MRM or PRM-MS

Targeted proteomics analysis by MRM or PRM-MS

Description: Relative or absolute quantification of protein panels by the monitorization of their peptides together with stable isotope labelled (SIL) standards.

Applications: This strategy is employed to verify or validate biological hypotheses with the highest specificity, as it detects the protein sequence and is not dependent on antibodies.

U30-S05 Targeted proteomics analysis by MRM or PRM-MS
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U30-S04. Quantitative shotgun proteomics by nLC-MS/MS

Quantitative shotgun proteomics by nLC-MS/MS

Description: Large-scale relative quantification of proteins in biological samples by nano-flow high performance liquid chromatography combined with mass spectrometry. It can use either data-dependent (DDA) or data-independent (DIA) acquisition modes. Quantification can be performed via label-free (LFQ) or protein/peptide isotopic labelling using SILAC, iTRAQ or TMT reagents. The output of this analyses are lists of proteins identified in the samples together with quantitative information obtained from the area displayed by each peptide in the mass spectra, and can then be compared across experimental conditions.

Applications: Protein profiling, biomarker discovery, drug discovery (protein target identification), development of precision medicine approaches, etc.

U30-S04 Quantitative shotgun proteomics by nLC-MS/MS
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U30-S03. Protein identification by mass spectrometry (MS) or MS/MS

Protein identification by mass spectrometry (MS) or MS/MS

Description: Identification of proteins in biological samples, either by intact protein analysis (MALDI-TOF) or protein digestion followed by peptide mass fingerprinting (PMF, MS) or peptide fragmentation (MS/MS).

Applications: Protein analysis, characterization of biological samples, analysis of protein interactions (e.g. pull-down assays).

U30 S03 Protein identification by mass spectrometry (MS) or MS/MS
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