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U12. Nanostructured liquid characterization unit – Equipment

U12. Nanostructured liquid characterization unit – Equipment

U12-E22. SEM Hitachi TM4000Plus II for morphological and dimensional characterization of solid samples

U12-E22. SEM Hitachi TM4000Plus II for morphological and dimensional characterization of solid samples

SEM Hitachi TM4000Plus II for morphological and dimensional characterization of solid samples.

Description:

Low vacuum Scanning Electron Microscopy with two electron detectors (secondary, SE, and backscattered, BSE). SE imaging provides a detailed information of the morphology of the sample and BSE gives information about the different components of the sample, based on the different atomic number (the higher the atomic number, the brighter the surface).

The equipment can apply different vacuum levels (using a turbo molecular pump) with accelerating voltages between 5 and 20 kV and magnifications from 10x to 100,000x. Specimens with size up to 80 mm diameter and/or 50 mm thickness can be characterized and samples.

Conductive and non-conductive samples can be characterized with little or no previous preparation. The software enables image processing of micrographs, for instance, measuring the size of solid particles after the acquisition of images.

Applications:

SEM analysis is very useful in several fields:

  • Life sciences, for the topographical and morphological characterization of tissues, hairs, fibers, etc.
  • Materials science, for quality control and failure analysis. Morphological properties are quite important in the research of innovative materials. In addition, the characterization of the surface is a key factor in porous materials used for delivery systems.
  • Semiconductor inspection and microchip assembly, to analyse the topography and to investigate the effectiveness of production methods.
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Design of parenteral MNP-loaded PLGA nanoparticles by a low-energy emulsification approach as theragnostic platforms for intravenous or intratumoral administration

Encapsulation of magnetic nanoparticles (MNP) into PLGA nanoparticles has been achieved by nano-emulsion templating using for the first time both, a low-energy emulsification method as well as biocompatible components accepted for pharmaceuticals intended for human use. The incorporation of MNP by nano-emulsion templating method proposed in this work has been investigated in two different systems applying mild process conditions and is shown to be simple and versatile, providing stable MNP-loaded PLGA nanoparticles with tunable size and MNP concentration. MNP-loaded PLGA nanoparticles showed sizes below 200 nm by DLS and 50 nm by TEM, and mean MNP loading per PLGA nanoparticle of 1 to 4, depending on the nanoparticle dispersion composition. Physical-chemical features suggest that the MNP-loaded PLGA nanoparticles obtained are good candidates for intravenous or intratumoral administration.

The equipment of Dynamic Light Scattering (Dynamic Light Scattering) of NANBIOSIS Unit 12 has been used to determine the size distribution of polymer particles and Microscopy with Hyperspectral Analysis (Hyperspectral enhanced dark field microscopy) to detect the encapsulation of magnetic nanoparticles in polymer particles.

Article of reference:

https://doi.org/10.1016/j.colsurfb.2017.09.060

Graphical abstract: Schematic representation of the preparation process of Fe3O4 MHighlightsN P-loaded PLGA nanoparticles by nano-emulsion templating.

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U12-E21. Fluorimeter Cary Eclipse (Varian)

U12-E21. Fluorimeter Cary Eclipse (Varian)

Fluorimeter Cary Eclipse (Varian) for fluorescence, phosphorescence, chemiluminiscence, bioluminiscence measurements.

U12-E21. Fluorimeter Cary Eclipse (Varian)
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U12-E20. Osmometer Type 15 (Löser Messtechnik)

U12-E20. Osmometer Type 15 (Löser Messtechnik)

Osmometer Type 15 (Löser Messtechnik) for osmolality measurements.

U12-E20. Osmometer Type 15 (Löser Messtechnik)
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U12-E19. HPLC 1525 Series (Waters)

U12-E19. HPLC 1525 Series (Waters)

HPLC 1525 Series (Waters)

U12-E19. HPLC 1525 Series (Waters)
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U12-E18. Viscosimeter RVDV-II+P (Brookfield)

U12-E18. Viscosimeter RVDV-II P (Brookfield)

Viscosimeter RVDV-II+P (Brookfield) for viscosity measurements.

U12-E18. Viscosimeter RVDV-II P (Brookfield)
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U12-E17. Zetasizer NanoZ (Malvern Instruments)

U12-E17. Zetasizer NanoZ (Malvern Instruments)

Zetasizer NanoZ (Malvern Instruments) for zeta potential measurements.

U12-E17. Zetasizer NanoZ (Malvern Instruments)
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U12-E16. Autosorb IQ (Quantachrome Instruments)

U12-E16. Autosorb IQ (Quantachrome Instruments)

Autosorb IQ (Quantachrome Instruments) for determination of surface area and pore volume.

U12-E16. Autosorb IQ (Quantachrome Instruments)
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U12-E15. SEM Hitachi TM-1000 (Hitachi)

U12-E15. SEM Hitachi TM-1000 (Hitachi)

SEM Hitachi TM-1000 (Hitachi) for morphological characterization.

U12-E15. SEM Hitachi TM-1000 (Hitachi)
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U12-E14. Avanti J30I ultracentrifuge (Beckman Coulter)

U12-E14. Avanti J30I ultracentrifuge (Beckman Coulter)

Avanti J30I ultracentrifuge (Beckman Coulter)

U12-E14. Avanti J30I ultracentrifuge (Beckman Coulter)
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