+34 679 490 537info@nanbiosis.com

U14. Cell Therapy

U14. Cell Therapy

New book by NANBIOSIS U14: Basic research procedures in the cell therapy laboratory

Researchers of NANIOSIS Unit 14 at JUMISC have published a new book titled Basic research procedures in the cell therapy laboratory, in which some basic techniques and general principles for adult stem cell research are explained.

Stem cells are cells capable of differentiating into a wide variety of cell types and can be isolated from different tissues such as. They have regenerative and anti-inflammatory properties thanks to the secretion of paracrine factors. Currently, these cells are being widely used in clinical trials for the treatment of different diseases such as Crohn disease, multiple sclerosis, diabetes, rheumatoid arthritis or myocardial infarction.
In this book, some basic techniques and general principles for working in a research laboratory in Cellular Therapy are explained in a simplified manner. In the same way, a series of basic knowledge are exposed that must be taken into account for the correct handling of the cells and their environment.
This book is not intended to be a detailed manual of laboratory procedures or a book on cell management for clinical use, but its purpose is to describe part of the protocols developed by the Cell Therapy Unit of NANBIOSIS at the Jesús Usón Minimally Invasive Surgery Center and which have the objective of evaluating the effectiveness and safety of these cells in the preclinical setting.

Book: Basic research procedures in the cell therapy laboratory. Álvarez Pérez, Verónica, García Casado, Javier, Sánchez-Margallo, Francisco.
ISBN 13: 978-84-17959-64-7

Read More

U14-S06. Lymphocyte proliferation assay (On-site&Remote) OUTSTANDING

Lymphocyte proliferation assay

Read More

U14-S05. Determination of cytokine concentration by flow cytometry (On-site&Remote) OUTSTANDING

Determination of cytokine concentration by flow cytometry

Read More

U14-S04. Cytokine quantification assays (On-site&Remote) OUTSTANDING

Cytokine quantification assays

Read More

U14-S03. Biomecanic evaluation of plantar pressure in large animal models (On-site) OUTSTANDING

Read More

U14-S02. Flow Cytometry (Onsite&Remote) OUTSTANDING

Cellular phenotyping, cytokine assays, profiferation assays, viability and apoptosis.

Read More

U14-S01. Cell cultures (Onsite&Remote) OUTSTANDING

Isolation and expansión of mesenchymal stem cells; viability assays and cytotoxicity, porcine mesenchymal stem cells (isolated from adipose tissue, bone marrow and synovial fluid) for preclinical trials. Murine mesenchymal stem (isolated from adipose and bone marrow).

Read More

U14-E01. Cell culture rooms

Cell Culture Room 1:
Vertical Laminar Flow Cabinet Herasafe® (Formimetal).
CO2 incubator Heracell 150 (Thermo)
Nomarski phase inverted microscope and fluorescencia con cámara (Nikon)
Camera Color 1CCD (Nikon)
Cooled Camera black and white (Nikon)
Vacuum Pump ME4 (Vacuubrand)
Cell culture water Baths (Controltecnica)

Cell Culture Room 2:
Vertical Laminar Flow Cabinet Herasafe® (Formimetal)
CO2 incubator Heracell 150 (Thermo)
CO2 incubator Heracell 150 with CO2 control (Thermo)
phases inverted microscope with fluorescence (NIKON)
Vacuum Pump ME4 (Vacuubrand)
Cell Counter (Invitrogen)

Read More

U14-E02. Molecular biology

Molecular Biology
Fume Hood (Formimetal)
Stereo microscope with camera and digital image set (NIKON)
light source (for the glass) (NIKON)
Refrigerated centrifuge for microtubs Biofuge® Pico (HERAEUS)
Digital circulation thermostatic bath (GRANT)
Thermostatic bath (for cell cultures temperar medios) (MEDIGEN))
Pipette controller pipette Powerpette-plus (IZASA)
Pipettes de volumen variable continuo with accessories (RAININ)
Vertical Freezer -20ºC (FIOCHETTI)
Freezer +2/+8ºC (CLASS ELECTRONIC)
Laboratory vertical freezer Herfreeze ®HFU-486-Basic (-80ºC) (HERAEUS)
Crioconservtion system with accessories
Liquid Nitrogen tank (taylor wharton)
Thermocycler (THERMO)
Sterilization incubator (Modelo UT ) (HERAEUS)
G-Box Translumitator and accessories (PC, Sofware…) (SYNGENE)
Electrophoresis system (IZASA)
Electrophoresis vertical jar
Electrophoresis horizontal (big) jar
Electrophoresis horizontal (small) jar
Rotary shaker with incubation (HEIDOLPH)
Magnetic stirrer with heating (ARE)
Analytical balance 210gr, 0,01 gr. (ACCULAB)
Precision analytical balance (OHAUS)
Ultrasonic water bath (SELECTA)
Crushed ice machine (Schottman)
Microwave (LG)
Pipette controller (POWERPETTE PLUS)
Thermoblock (Labolan)
Vortex 230 V, EU (VWR)
Freezer +4ºC/-20ºC
UV mini-1240 Spectrophotometer (SHIMADZU)
ELISA plate reader, Synergy MX (BIOTEK)
Flow cytometry FACSCalibur (BD)
pH-Meter (Symphony)
i-Blot (Invitrogen)
Autoclave

Read More