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U20. In Vivo Experimental Unit – Equipment

U20. In Vivo Experimental Unit – Equipment

U20-E04 General laboratory equipment

General laboratory equipment

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U20-E03. Animal Facility

Animal Facility:

The VHIR’s Animal Facility includes areas for rodents, rabbits, pigs, minipigs and sheeps. In the case of rodents the facility includes:
– Standard clean area and passive quarantine with an area of 136 m2 with
-10 holding rooms for 225 rats and 11,200 mice
-1 passive quarantine and isolation room with modern microisolators and capacity for 560 mice
-6 handling areas for carrying out experimental and surgical procedures with supplies of gases and inhalable anesthesia as well as surgical microscopes
– A Barrier area with 65 m2 for housing and handling of specific pathogen free (SPF) animals, including immunodeficient mice
-The Molecular Imaging Platform Room, where the molecular imaging equipment is located

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U20-E02. Equipment for histological processing

Equipment for histological processing:

The facility has the equipment necessary for fixation(chemical or by freezing), preparations of blocks, slicing and staining using various techniques, from conventional to immunohistochemical stains. The set-up includes everything from the automatic tissue processor to a paraffin embedding station, two microtomes, anautomated staining systems and a microscope for digital registration of the samples.

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U20-E01. Optical imaging equipment

Optical imaging equipment:

– Xenogen IVIS ® Spectrum: is a high sensitivity, low background noise system for non-invasive optical imaging which enables bioluminescence and fluorescence imaging of cells (in vitro), entire animals (in vivo) and organs (ex vivo).

– Leica MacroFluo™: is a precision macroscope with filters for multiple fluorochromes, a Z16 APO (16:1) zoom system and a CCD camera to monitor and record the fluorescence of entire organs and tissues at high resolution with no need to process them histologically.

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