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U6. Biomaterial Processing and Nanostructuring Unit

U6. Biomaterial Processing and Nanostructuring Unit

U6-S06. Fluorescence spectroscopic analysis (On-site&Remote)

Sample analysis by the equipment

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U6-S07. UV-Vis spectroscopic analysis (On-site&Remote)

Sample analysis by the equipment

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U6-S08. Visual observation (On-site&Remote)

Visual observation by an optical microscope ( image processing, optical microscopy).

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U6-S09. Centrifugation (On-site)

Centrifugation of samples

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U6-S02. High-pressure phase analysis – solubility, emulsification (Remote) OUTSTANDING

For characterization of the biomaterial

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U6-S01. Use of High-pressure laboratory-scale plant with 50, 100 and 300 m reactors for the processing of biomaterials. Processing of cytotoxic compounds when required. (Remote) OUTSTANDING

Preparation of micro- and nanostructured materials, using compressed fluids as Green solvents (high pressure plants from 30 to 300 mL).

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U6-E05. Nanosight NS-300 for Nanoparticle Tracking Analysis by fluorescence mode

Nanosight NS300; Malvern Instruments

Descripción: Automated analysis of the size distribution and concentration

The Malvern NanoSight NS300 uses the technology of Nanoparticle Tracking Analysis (NTA). This unique technology utilizes the properties of both light scattering and Brownian motion in order to obtain the size distribution and concentration measurement of particles in liquid suspension. A laser beam is passed through the sample chamber, and the particles in suspension in the path of this beam scatter light in such a manner that they can easily be visualized via 20x magnification microscope onto which is mounted a camera. The camera operates at 30 frames per second (fps), capturing a video file of the particles moving under Brownian motion. The software tracks many particles individually and using the Stokes-Einstein equation calculates their hydrodynamic diameters.

Location: Laboratory SCF.  (MATGAS)

More info:  http://www.malvern.com/en/products/product-range/nanosight-range/nanosight-ns300/default.aspx

Especificaciones técnicas:

Wavelength: 405nm (violet), 488nm (blue), 532nm (green) or 642nm (red).

Temperature control range: 5°C below ambient to 55°C.

Stage: Fixed stage.

Focus: Computer controlled motorized focus.

Camera:

Fluorescence: Motorized 6 place filter wheel with choice of filters.

Particle size: 10nm to 2000nm.

Concentration range: 106to 109 particles per mL.

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U6-S14. Quantitative studies of biomolecular interactions by calorimetric measurements (On-site) OUTSTANDING

Quantitative studies of biomolecular interactions by calorimetric measurements.

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U6-S13. Detection of labeled nanoparticles and exosomes by fluorescence mode (On-site&Remote) OUTSTANDING

Detection of labeled nanoparticles and exosomes by fluorescence mode.

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