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In vitro Characterization of Nanomedicines

Phalloidin and DAPI staining of human pigmented epithelial cells (ARPE cells)
Phalloidin and DAPI staining of human pigmented epithelial cells (ARPE cells).

Scientific Leaders of the project: Javier García, José Luis Pedraz, Simó Schwartz & Ramón Mangues.
Coordinator of the project: Ibane Abasolo & Javier García

Industrial problem/gap covered

One of the causes that increase the time to market of nanotherapeuticsis its preclinical validation. The standard protocols for more traditional drugs are not applicable for nanomedicines. NANBIOSISoffers a preclinical study plan tailored to meet the demands of each nanomedicine product, developed in collaboration with the user and starting from a set of basic analytical tests to more sophisticated experiments.


We offer a complete In vitro Characterization. e.g., immunology, cytotoxicity, hematology, oxidative stress, etc, and more adapted to the different products by using the most sophisticated equipment and taking advantage of the expertise of scientist internationally recognized in the matter.

Some examples are described in the following publications:

Sodiumcolistimethate loaded lipid nanocarriers for the treatment of Pseudomonas aeruginosa infections associated with cystic fibrosis. M. Pastor. M. Moreno-Sastre, A. Esquisabel, E. Sans, M. Viñas, D.lBachiller, V. J. Asensio, Á. Del Pozo, E. Gainza, J. L. Pedraz. Int. J. of Pharm. 477 (2014) 485-494.

Intrapericardial Delivery of Cardiosphere-Derived Cells: An Immunological Study in a Clinically Relevant Large Animal Model. Blázquez R, Sánchez-Margallo FM, Crisóstomo V, Báez C, Maestre J, Álvarez V, Casado JG. PLoS One. 2016 Feb 11;11(2):e0149001. doi: 10.1371/journal.pone.0149001. eCollection 2016.

Immunomodulatory Potential of Human Adipose Mesenchymal Stem Cells Derived Exosomes on in vitro Stimulated T Cells. Blazquez R, Sanchez-Margallo FM, de la Rosa O, Dalemans W, Alvarez V, Tarazona R, Casado JG. Front Immunol. 2014 Nov 4;5:556. doi: 10.3389/fimmu.2014.00556. eCollection 2014.

Services involved:

Sterility Gel-Clot LAL Assay U20
Detection of Microbial Contamination U20
Detection of Bacterial Contamination U20
Detection of Mycoplasma Contamination U20
Immunology Analysis of Hemolytic Properties of Nanoparticles U20
Analysis of Platelet Aggregation by Cell Counting U20
Analysis of Platelet Aggregation by Light Transmission Aggregometry U20
Analysis of Nanoparticle Interaction with Plasma Proteins by 2D PAGE U20
Leukocyte Pro life ration Assay U14
Detection of Nitric Oxide Production by RAW 264. 7 MacrophageCell Line U18
ChemotaxisAssay U18
PhagocytosisAssay U18
Analysis of Cytokines, Chemokines and Interferons (by Elisa or Multiplex) IL-8, IL-1b, TNF-α, IFN- ϒ and many others U14
Determination of cytokine concentration by flow cytometry U14
Measurement of Nanoparticle Effects on Cytotoxic Activity of NK Cells by Label-Free RT-CES System U14 (consult)
In vitro Induction of Leukocyte Procoagulant Activity by Nanoparticles U20 (consult)
Oxidative Stress Hep G2 Hepatocyte Glutathione Assay U20
Hep G2 Hepatocyte Lipid Peroxidation Assay U20 (consult)
HepatocytePrimary ROS Assay U20 (consult)
Cytotoxicity (necrosis) Cytotoxicity (necrosis) Assay (MTT and LDH Release): LLC-PK1 Kidney, Hep G2 Hepatocarcinoma, etc U10, U18 & U20
Cytotoxicity (apoptosis) Cytotoxicity (apoptosis) (Caspase 3 Activation) LLC-PK1 Kidney; Hep G2 Hepatocarcinoma ; etc U10 U18
Cytotoxicity (apoptosis) (Caspase 3/7 Activation) Hep G2 Hepatocarcinoma ; etc U10 & U18
Autophagy Autophagic Dysfunction Assay: Qualitative Analysis of MAP LC3I to LC3-II Conversion by Western Blot U18
Autophagic Dysfunction in LLC-PK1 Cells U18
Immunology Lymphocyte proliferation U14
Detection of Antibodies (anti PEG and others) U2
Cell uptake By cytometry U14, U18 & 20
By Confocal Microscopy U2
Of metallic NPs/Fe-staining U20
Optical Analysis: HR Dark-Field optical microscope with spectral analysis(Nano-scale Optical Microscope) U12


Effect of NP son cell viability and distribution in the cell cycle.

Elisa plate.

Phalloidin and DAPI staining of human pigmented epithelial cells (ARPE cells).