Flow cytometry is a highly effective method for analyzing thousands cells or other particles in a matter of minutes. Flow cytometers detect the fluorescence, size and complexity of particles that pass through laser beams as a stream. In this way they can quickly generating statistically valid data by count very large numbers of particles – something that would be extremely difficult with traditional microscopy approaches. Flow cytometers are very well established in hospitals for routine diagnosis and now widely used for different types of research application from marine biology to stem cell research. A Cell Sorter is a specialized flow cytometer which is able to physically separate different classes of particles based on their fluorescence or other physical characteristics.
>> Detection of rare events: the high throughput of Gallios allows for the statically-valid detection of very rare cellular events.
>> Multi-parameter assays: Three lasers and ten channels of Gallios allows the design of complex assays using multiple markers.
>> Cell cycle analysis: detection of DNA content, DNA damage, apoptosis and cell cycle antigens and many others.
>> Marine biology: flow cytometry is increasingly used as a tool to study marine microorganisms using their innate fluorescence properties combined with size and complexity characteristics.
>> Single cell separation: Mo-Flo is able to automatically sort single cells into individual wells of a 96-well plate with a very high degree of accuracy and is suitable for colony formation, stem cell isolation etc.
>> Cell purification from tissues: Mo-Flo allows individual cell types from complex tissues to be separated based on size, complexity or markers such as GFP. The yield and purity can be adjusted to suit different applications. Measurement of Relaxation Times (T1, T2, T1*)