+34 620 10 75 37info@nanbiosis.com

U1. Protein Production Platform (PPP)

U1. Protein Production Platform (PPP)

U1-S05. Training courses in protein production (Biomolecules production)

Training courses in protein production (Biomolecules production)

The PPP unit provides personalised training courses in recombinant protein production and purification either at our facilities or at the client’s.
The courses can include practical training sessions, for example, in the use of FPLC-AKTA systems.

Examples of trainings are:

  • “Strategies for optimization of recombinant protein production” Advanced and Initial levels.
  • Theoretical-practical training in the management of the FPLC-AKTA system.

Applications: For users who want to use FPLC-AKTA systems. For staff of biotechonology companies. For students of Ph.D. programs.

Customer benefits

The PPP Unit specialises in designing, producing, and purifying recombinant proteins on demand, tailored to customers‘requirements. We have an extensive expertise in designing different strategies to achieve successfully final products according customers‘ needs. The service is completely personalised to ensure that the training fits the needs of the costumer. The training courses can be made collectively or individually.

Target customer

The PPP Unit extends its services across the scientific community, serving both private and public research organizations. This includes support for research centres, universities, hospitals, and companies in the sector. Leveraging our connection with the university, the PPP unit also provides specialised courses as part of the official master’s degrees and PhD programs at UAB. The training courses can be made collectively or individuality.

Read More

U1.E05. Tangential filtration Unit for purification

Tangential filtration Unit for purification.

U1.E05_TANGENTIAL FILTRATION UNIT FOR PURIFICATION_1
Read More

U1.E04. FPLC ÄKTA Chromatography systems for purification

ÄKTA pure™ is a flexible and intuitive chromatography system for fast purification of proteins, peptides, and nucleic acids from microgram levels to tens of grams of target product.

ÄKTA pure™ chromatography system provides you with the following benefits:
• Modular system design with a large range of options to allow flexibility in purification of proteins and peptides
• Intuitive and flexible method creation, system control, and evaluation with UNICORN™ software
• Practical size, for easy placement on laboratory bench or in cold cabinet
• Reliable system with components and integrated
features based on the proven design of ÄKTA™ protein purification systems
• Predefined method settings for all our laboratory-scale chromatography columns

A double-door refrigerator (Unichromat 1500 HPLC cooling cabinet (UNIEQUIP) is available to contain FPLC-AKTA purification equipment inside, refrigerated, to be able to carry out low-temperature purifications of proteins that are sensitive to room temperature.

It also has a probe for acquiring temperature data from the refrigerator, to be able to track at all times the status of the samples that are stored inside the services during the protein production and purification service process and after

.

ÄKTA Purifier equipment for protein purification
Read More

U1.E03. Incubator shaker with cooling unit for insect cells suspension cultures

Incubator shaker with cooling unit for insect cells suspension cultures.

U1.E03_INCUBATOR SHAKER WITH COOLING UNIT FOR INSECT CELL SUSPENSION
Read More

U1.E02. 10 liter WAVE bioreactor for insect cells

10 liter WAVE bioreactor for insect cells.

U1.E02_10 LITER WAVE BIOREACTOR FOR INSECT CELLS
Read More

U1.E01. 2 and 7 liter bioreactors for bio production

2 and 7 liter bioreactors for bio production

U1.E01_2 AND 7 LITER BIOREACTORS FOR BIO PRODUCTION_1
Read More

U1-S06. Criopreservation

Criopreservation

The PPP unit offers the oportunity to cryopreserve client samples under carefully controlled conditions using a temperature control system. This system notify us of any equipment malfunctions or temperature fluctuations that may affect the samples.

Customer benefits

For customers that don’t have equipment/space or control systems to store their samples adequately.

Target customer

The PPP Unit extends its services across the scientific community, serving both private and public research organizations. This includes support for research centres, universities, hospitals, and companies in the sector.

Read More

U1-S04. Consultancy in protein production

Consultancy in protein production

The PPP unit provides consultancy taking into acount specific issues covering cloning, recombinant protein production, and purification strategies.
The consultancy covers the entire design process for obtaining recombinant proteins according to specific needs, as well as troubleshooting solutions for achieving soluble and stable active proteins.

Examples of consultancy include:

  • Cloning: Expression vector, DNA sequence, optimization, tags, etc.
  • Protein production: expression system, optimization, etc.
  • Protein purification: general strategy, tags, buffers, etc.
  • Protein stability: storage conditions (buffer, temperature, format)

Customer benefits

The PPP Unit specialises in designing, producing, and purifying recombinant proteins on demand, tailored to customers‘ requirements. We have an extensive expertise in designing different strategies to achieve succesfuly final products acccording to customers‘ needs. The service is completely personalised to ensure that the final product matches the intended use in terms of format, purity and activity.

Target customer

The PPP Unit extends its services across the scientific community, serving both private and public research organizations. This includes support for research centres, universities, hospitals, and companies in the sector. Leveraging our connection with the university, the PPP unit also provides specialised courses as part of the official master’s degrees and PhD programs at UAB.

Read More

U1-S03. Proteins purification

Proteins purification

The PPP Unit counts with a laboratory perfectly equipped for the purification of recombinant proteins. The protein purification procedure is carried out according the protein characteristics and customers‘ needs.
Design: identification of the most appropriate strategy for protein purification, given the physical and chemical properties, opting either for purification modules or, in the case of fusion proteins, elements which enable purification by affinity.
Protein purification: downstream processing using FPLC chromatography and/or tangential filtration either from the cellular pellet or the supernatant when the protein is secreted. When necessary, the purification is performed through solubilization of inclusion bodies or by purification under denaturing conditions with later refolding.
Final product: the purified protein is delivered in the buffer, temperature and the concentration requested by the costumer. When demanded, the protein can be delivered in lyophilised form.
Quality control: the purified recombinant protein is analysed in terms of molecular weight, purity by SDS-PAGE, aggregation by DLS and identification by Peptide-Mass Fingerprinting. Additional analysis can be performed under customer’s agreement such as buffer optimization (Microdyalisis) or freeze-thaw stability.

Customer benefits

The service is completely personalised to ensure that the final product matches the intended use in terms of format, purity and activity. We have various purification methodologies to address specific needs, offering a wide range of possibilities for obtaining the final product. All services adhere to standardised procedures, guaranteeing the quality of the end product. Thus, instead of offering a generic solution, we deliver a customised product with the exact specifications requested by the customer.

Target customer

The PPP Unit extends its services across the scientific community, serving both private and public research organizations. This includes support for research centres, universities, hospitals, and companies in the sector. Leveraging our connection with the university, the PPP unit also provides specialised courses as part of the official master’s degrees and PhD programs at UAB.

Additional information

Over its 17-year history, the PPP unit has successfully completed over 400 projects and has been recognised in more than 121 scientific publications. In the past three years, it has received citations in the following works:

  • ACS Materials Lett. 2024, 6, 3, 954–962
  • Front. Immunol. 2024. 15:1346512.
  • Applied Microbiology and Biotechnology (2024) 108:98
  • ACS Sustainable Chem. Eng. 2023, 11, 4133−4144
  • Pharmaceutics 2023, 15, 2632.
  • Microbial Cell Factories (2023) 22:81
  • Biomed Pharmacother 2023. 164:114976.
  • ACS Appl. Mater. Interfaces 2023, 15, 39167−39175
  • Pharmaceutics 2023, 15, 1197
  • Sci China Mater 2023, 66(10): 4109–4120
  • Pharmaceutics 2023, 15(3), 727
  • Biomedicine & Pharmacotherapy 2023, 169(1):115848
  • Appl Environ Microbiol 2023 Jun 28;89(6)
  • Biomedicine & Pharmacotherapy 150 (2022) 112940
  • Biomaterials 280 (2022) 121258
  • Pharmaceutics 2022, 14, 887
  • J. Exp Clin Cancer Res (2022) 41:49
  • DRUG DELIVERY 2022, VOL. 29, NO. 1, 1384–1397
  • Biomedicines 2022, 10, 1680.
  • Microbial Cell Factories (2022) 21:203
  • Acta Pharmaceutica Sinica B 2022;12(5):2578e2591
  • Pharmaceutics 2022, 14, 602.
  • Pharmaceutics 2022, 14, 192.
  • Front. Bioeng. Biotechnol. 2022 10:842256.
  • Anal. Chem. 2022, 94, 5359−5366
  • Odontology (2022) 110:545–556
Read More

U1-S02. Bioproduction of proteins

Bioproduction of proteins

The PPP Unit counts with a laboratory perfectly equipped for the bioproduction of recombinant proteins. The expression system is selected according to the protein characteristics and customer’s needs.
Transformation/transfection: the engineered construct is introduced inside the chosen expressing cells. In certain instances, involving insect expression, this step includes the isolation of a bacmid made for protein production via the baculovirus system.
Optimisation: selection of the optimal protein production conditions using small and medium-scale expression; confirmation of the protein expression using SDS-PAGE and Western blot analysis and quantification by densitometry. At this step protein solubility/aggregation is measured to obtain the best solubilizing conditions.
Recombinant protein production: scale-up of the upstream processing by batch culture according to the best protein production conditions selected during the optimization step.

Customer benefits

The service is completely personalised to ensure that the protein matches the intended use for later protein purification. Thus, instead of offering a generic solution, we deliver a customised product with the exact specifications and needs requested by the customer.
We have various expression systems to address specific needs, offering a wide range of possibilities for obtaining the final product. All services adhere to standardised procedures, guaranteeing the quality of the end product.

Target customer

The PPP Unit extends its services across the scientific community, serving both private and public research organizations. This includes support for research centres, universities, hospitals, and companies in the sector. Leveraging our connection with the university, the PPP unit also provides specialised courses as part of the official master’s degrees and PhD programs at UAB.

Additional information

Over its 17-year history, the PPP unit has successfully completed over 400 projects and has been recognised in more than 121 scientific publications. In the past three years, it has received citations in the following works:

  • ACS Materials Lett. 2024, 6, 3, 954–962
  • Front. Immunol. 2024. 15:1346512.
  • Applied Microbiology and Biotechnology (2024) 108:98
  • ACS Sustainable Chem. Eng. 2023, 11, 4133−4144
  • Pharmaceutics 2023, 15, 2632.
  • Microbial Cell Factories (2023) 22:81
  • Biomed Pharmacother 2023. 164:114976.
  • ACS Appl. Mater. Interfaces 2023, 15, 39167−39175
  • Pharmaceutics 2023, 15, 1197
  • Sci China Mater 2023, 66(10): 4109–4120
  • Pharmaceutics 2023, 15(3), 727
  • Biomedicine & Pharmacotherapy 2023, 169(1):115848
  • Appl Environ Microbiol 2023 Jun 28;89(6)
  • Biomedicine & Pharmacotherapy 150 (2022) 112940
  • Biomaterials 280 (2022) 121258
  • Pharmaceutics 2022, 14, 887
  • J. Exp Clin Cancer Res (2022) 41:49
  • DRUG DELIVERY 2022, VOL. 29, NO. 1, 1384–1397
  • Biomedicines 2022, 10, 1680.
  • Microbial Cell Factories (2022) 21:203
  • Acta Pharmaceutica Sinica B 2022;12(5):2578e2591
  • Pharmaceutics 2022, 14, 602.
  • Pharmaceutics 2022, 14, 192.
  • Front. Bioeng. Biotechnol. 2022 10:842256.
  • Anal. Chem. 2022, 94, 5359−5366
  • Odontology (2022) 110:545–556
Read More