U1. Protein Production Platform (PPP)

e.g. Course on “Strategies for optimization of recombinant protein production”)

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Tangential filtration Unit for purification.

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FPLC ÄKTA Chromatography systems for purification

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Incubator shaker with cooling unit for insect cells suspension cultures.

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10 liter WAVE bioreactor for insect cells.

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2 and 7 liter bioreactors for bio production

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Criopreservation of Proteins, Plasmids, etc

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In all matters dealing with protein production, expresion systems, etc, according to the final application of the product.

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Identification of the most appropriate strategy for purification, given the physical and chemical properties, opting either for purification modules or, in the case of fusion proteins, elements which enable purification by affinity.
Purification (downstream processing) using FPLC chromatography and/or tangential filtration.
Quality control of the purified protein ( molecular weight and purity by SDS-PAGE, biological activity, conformation by FTIR and DLS.

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Production of the plasmid DNA that contains the gene of the relevant protein to be incorporated into the producing cells by transformation/transfection.
Selection of the optimal protein production conditions using small and medium-scale expression; confirmation of the expression using Westernblot and SDS-PAGE analysis and quantification by densitometry.
Measurements of the solubility/aggregation of the protein under study.
Scale-up of the upstream processing in bioreactors or incubator shaker according to requirements.”

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