U26. NMR: Biomedical Applications II – Equipment

U26-E06. Gilson 215 liquid handler for automated sample preparation of liquid samples

U26-E04. Bruker DPX300 spectrometer(7 T) with standard entrance, and two RF channels.

Accessories:

• 5mm QNP (1H, 13C, 31P and 19F), with pneumatic accessory for automatic nucleus change and field gradients along the z axis.
• BACS autosampler for 60 samples.

U26-E02. -NMR microscopy system for imaging Bruker 14T

• Functional and metabolic studies in cells, tissues and animal models. Coils 2mm, 5mm and 10mm. Image of 1H, 13C and 31P.
• Microcamera for its use in the NM R equipment for:
• The simultaneous determination of structural images (dimensions and sections of 50 μm and less)
• Metabolic images (voxel sizes < 200 μm) in model systems.

U26-E05 Bruker Avance III 400 WB spectrometer, with ultrashielded widemouth magnet (89 mm), 400 MHz (9.4 T), with two RF channels and high power amplifiers for solid-state experiments.

Accessories:

• DVT 1H/BB Probe. 4mm multinuclear probe, with 1H channel and broadband channel with range frequency from 15N to 31P.
• DVT 1H/BB Probe. 7mm multinuclear probe, with 1H channel and broadband channel with range frequency from 15N to 31P.
• 2H lock channel for observing liquids.
• PA BBOF Probe. 5mm direct multinuclear probe with broadband channel (range frequency from 15N to 31P) and additional observation of 19F.

U26-E03. Bruker DRX500 spectrometer (11.7 T), and with three RF channels

11T NMR equipment with the following probes:

•• HR-MAS,
•• Cryoprobe, and
•• multinuclear probe with service for metabolomics for tissues, cell lines and biofluids.

Accessories:

• BBOF Plus ATM. 1H/BB/19F direct multinuclear probe (5mm), with field gradients along the z axis, and automatic frequency tuning. Range of frequencies in the broadband channel from 15N to 31P.
• TBI. 1H/BB/15N triple reverse probe (5mm), with field gradients along the z axis. Range of frequencies in the broadband channel from 15N to 31P.
• BACS autosampler for 60 samples.
• Accessory to control temperature, with air down to 5ºC. Liquid nitrogen evaporator for low temperature experiments.

U26-E01. DRX-600 Bruker NM R equipment (14 Teslas)

DRX-600 Bruker NM R equipment (14 Teslas):

•• 4-channel electronic systems with applications in fluids, tissues, biomaterials and microimaging
•• Thermostating system for fluids and tissues, and for molecular imaging and microimaging
•• Set of coils: fluids 5 mm, micro-fluids 1 mm, tissues (High Resolution Magic Angle Spinning, HR-MAS) + pneumatic and microimaging system for 10, 5 and 2 mm.
•• 60 Amp GREAT unit for each X/Y/Z axis of the field gradients
•• 13C selective device with 1H decoupling.
•• 2D microcoil for 500 μm and 100 μm microsamples.
•• Refrigerated SampleJet autosampler with temperature control for 60 Samples