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Biomolecules production - Services

Biomolecules production – Services

U29-S02. Modification of oligonucleotides. (Remote) OUTSTANDING

Modification of oligonucleotides during and post synthesis to meet user requirements:

  • Conjugation of oligonucleotides with fluorophores (fluoresceine, Cy3, Ct5, etc..) and other types of small molecules such as biotine, lipids.
  • Conjugation to peptides.
  • Phosphorothioate linkages
  • Modified backbones such as locked nucleic acids (LNA), 2’-O-alkyl-RNA, etc..
  • Modified nucleobases: 2-aminopurine, 5-methyl-dC, etc…
  • 5’, 3’-modifications such as 5’-, 3’-amino, 5’-, 3’-thiol, etc..
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U29-S03. Special nucleotides for oligonucleotide synthesis. (Remote) OUTSTANDING

Special nucleotides for oligonucleotide synthesis

For those services identified as outstanding, at least 20% of their capacity is open under competitive access. See Annex 1 of ACCESS PROTOCOL (provided by Nanbiosis) for details on % of openness for each service

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U29-S01. Synthesis of oligonucleotides and characterization (On-site & Remote). OUTSTANDING

Synthesis of oligonucleotides at various different scales (100 microg to 5 mg) and purification using HPLC and/or desalting.

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U28-S07. Transmission Electron Microscopy (TEM) (Onsite&Remote)

Conventional TEM provides structural information of samples prepared to electron transparency in the sub-nanometer range. Once prepared, many biological samples, including bacteria, viruses, yeasts, cells, and tissue sections can be characterized using TEM. Moreover, TEM can be used to characterize many other types of material such as substrates or nanoparticles making it a highly versatile technique.

Applications:
>> Cellular characterization
>> Tissues characterization
>> Biomaterials characterization
>> Macromolecular complexes characterization

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U3-S04. Peptide libraries (Remote) OUTSTANDING

Peptide libraries.

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U3-S03. Special amino acids for peptide synthesis (Remote) OUTSTANDING

Special amino acids for peptide synthesis.

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U2-S08. Inmunochemical methods development (Remote) OUTSTANDING

CAbS offers personalized development of immunoassays. Antibodies and other necessary immunoreagents can be synthesized.

The development of the immunoassay includes different stages:

  1. Design of the immunoassay format, sample matrix, the sensibility and the working range. Identification of necessary reagents and their conditions to obtain optimal results.
  2. Development Phase. Establishing assay parameters, for example the detection limit and working range. Evaluation of the effects of the matrix on the assay. Reproducibility study.
  3. Assay validation. Evaluation of the stability and robustness of the reagents. Measurement of inter and intra-assay reproducibility, precision and specificity. Validation with blind samples. Assay platforms: ELISA, microarrays with different formats based on the detection of individual analytes or multiplexed assays.
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U2-S07. Antibody purification (Remote) OUTSTANDING

The purification of the antibodies includes the isolation of the antibody from the serum (polyclonal antibodies) or culture supernatant from hybridoma cell lines (monoclonal antibodies). The services offers previous advise on the different possibilities for purification according to the needs of the user.

The methods of purification that we offer vary from crude methods (precipitation of the proteins from the sample including any antibody present) to general purification (purification by affinity of certain classes of antibodies without taking account of the specificity to the antigen) to specific (purification by affinity of the antibodies which join specifically to a definite antigen).

  • Ammonium sulfate precipitation
  • Protein A, protein G affinity purification
  • Specific antigen affinity purification
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U2-S06. Hybridoma cell culture antibody production (Remote) OUTSTANDING

Hybridoma cell culture in ultralow serum medium. We use CELLine™  Bioreactor Technologys to produce monoclonal antibodies.

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U2-S05. Monoclonal Antibody development (Remote) OUTSTANDING

The mAb development contains the following phases:

  1. Immunisation of mice, titre determination in the antiserum.
  2. Cultivation of the myeloma cell line, cell fusion, selection in HAT medium and screening.
  3. Cloning , screening and cultivation of positive cultures. Further cloning and obtaining stable clones.
  4. Expansion and Cryo conservation of the selected clones. Delivery of cell culture supernatants and frozen hybridoma cells.
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