U1-S02. Bioproduction of proteins (On-site&Remote) OUTSTANDING
- Production of the plasmid DNA that contains the gene of the relevant protein to be incorporated into the producing cells by transformation/transfection.
- Selection of the optimal protein production conditions using small and medium-scale expression; confirmation of the expression using Westernblot and SDS-PAGE analysis and quantification by densitometry.
- Measurements of the solubility/aggregation of the protein under study.
- Scale-up of the upstream processing in bioreactors or incubator shaker according to requirements.”