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Equipments

Equipments

U20-E01. Optical imaging equipment

Optical imaging equipment:

– Xenogen IVIS ® Spectrum: is a high sensitivity, low background noise system for non-invasive optical imaging which enables bioluminescence and fluorescence imaging of cells (in vitro), entire animals (in vivo) and organs (ex vivo).

– Leica MacroFluo™: is a precision macroscope with filters for multiple fluorochromes, a Z16 APO (16:1) zoom system and a CCD camera to monitor and record the fluorescence of entire organs and tissues at high resolution with no need to process them histologically.

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U22-E06. Room for receipt and handling of animals

One hall for the reception and handling of animals.

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U22-E07. Quarantine rooms pre-experimental process

2 quarantinerooms pre-experimental process

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U22-E08. Room to perform small procedures and cures and preparation rooms for large animals prior to the surgical area

Room to perform small procedures and cures and preparation rooms for large animals prior to the surgical area

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U22-E05. Multi-use rooms for rodents and lagomorphs maintenance

Four multi-use rooms for maintenance of rodents and lagomorphs.
Lagomorphs can individually accommodate upto 102 rabbits.

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U22-E04. Integrated operating room with in the barrier area

One barrier zone for rodents maintenance under SPF conditions with capacity for about 3,000 animals in microisolators. It has autoclaves, SAS to the material inlet, automatic beverage system by reverse osmosis, laminar flow hoods for animal handling and integrated operating room within the barrier zone.

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U22-E03. Barrier zone for maintenance of rodentsunder SPF conditions

One barrier zone for rodents maintenance under SPF conditions with capacity for about 3,000 animals in microisolators. It has autoclaves, SAS to the material inlet, automatic beverage system by reverse osmosis, laminar flow hoods for animal handling and integrated operating room within the barrier zone.

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U22-E01. Rooms for the maintenance of large animals

This unit has facilities with a perimeter area of 3,200 m2 and it has large rooms to house the experimental animals according to their species characteristics. Among the equipment and facilities include:
9 rooms for large animals (pigs) maintenance with capacity for about 350 animals, for long periods of time and environmental, nutritional and microbiological conditions controlled.

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U22-E02. Rooms for maintaining small ruminants

2 rooms for the maintenance of small ruminants (sheep and goats) with capacity for about 60 animals over long periods of time and environmental, nutritional and microbiological conditions controlled.

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U17-E01. Leica TCS-SP5 confocal microscope

Leica TCS-SP5 confocal microscope with especial features that allows studying interactions between cells/tissues and materials. Indeed, the experience of the research group in charge of this Unit makes this a unique service for the study of cells and tissues and the interactions between various materials and cell components as well as between implants/scaffolds and tissues of the recipient organism. For example, it makes it possible to characterize in detail the specific markers of particular cell populations for tissue engineering applications and in vitro tests of biocompatibility of new materials, allowing the process of cell colonization of surfaces to be explored and simultaneous analysis of the cells or subcellular structures and materials. The combined use of fluorescence and reflection makes it possible to study the tissue-implant interface, with up to 8 fluorophores, at wavelengths from 405 to 633 nm allowing histological studies of a wide range of tissue types which would be restricted at certain fixed wavelengths due to autofluorescence phenomena.

 

Leica TCS-SP5 confocal microscope with:

DMI 6000 inverted microscope with 4 objectives:
10X (dry); 20X; 40X and 63X(for immersion).

Confocal module:
•• 3 channels for spectral detection
•• AOBS (Acousto Optical Beam Splitter)
•• Resonant scanning system.

4 lasers:
•• Argon laser (excitation at 458, 477, 488, 496 and 514 nm)
•• He/Ne laser (excitation at 633 nm)
•• DPSS (diode-pumped solid-state) laser (excitation at 561 nm)
•• UV laser (excitation at 405 nm)

Incubation chamber on a motorized stage.

Workstation with 4 types of software for data acquisition and processing:

•• 3D imaging
•• Colocalization analysis
•• FRAP (Fluorescence Recovery After Photobleaching)
•• FLIP (Fluorescene Loss In Photobleaching)
•• FRET (Fluorescence Resonance Energy Transfer)

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