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Equipments

Equipments

U12-E22. SEM Hitachi TM4000Plus II for morphological and dimensional characterization of solid samples

U12-E22. SEM Hitachi TM4000Plus II for morphological and dimensional characterization of solid samples

SEM Hitachi TM4000Plus II for morphological and dimensional characterization of solid samples.

Description:

Low vacuum Scanning Electron Microscopy with two electron detectors (secondary, SE, and backscattered, BSE). SE imaging provides a detailed information of the morphology of the sample and BSE gives information about the different components of the sample, based on the different atomic number (the higher the atomic number, the brighter the surface).

The equipment can apply different vacuum levels (using a turbo molecular pump) with accelerating voltages between 5 and 20 kV and magnifications from 10x to 100,000x. Specimens with size up to 80 mm diameter and/or 50 mm thickness can be characterized and samples.

Conductive and non-conductive samples can be characterized with little or no previous preparation. The software enables image processing of micrographs, for instance, measuring the size of solid particles after the acquisition of images.

Applications:

SEM analysis is very useful in several fields:

  • Life sciences, for the topographical and morphological characterization of tissues, hairs, fibers, etc.
  • Materials science, for quality control and failure analysis. Morphological properties are quite important in the research of innovative materials. In addition, the characterization of the surface is a key factor in porous materials used for delivery systems.
  • Semiconductor inspection and microchip assembly, to analyse the topography and to investigate the effectiveness of production methods.

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U29-E06. UV-Visible and luminometer Reader, multidetection system.

UV-Visible and luminometer Reader used in luciferase and MTT assays

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U29-E07. Electrophoresis apparatus with several vertical and horizontal cuvettes.

Electrophoresis apparatus with several vertical and horizontal cuvettes for agarose and polyacrylamide (PAGE) gel electrophoresis.

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U29-E01. Automatic synthesizer.

Automatic synthesizer which can be operated at a range of scales (40, 200, 1000 nanomols). A large variety of monomers can be incorporated using the phosphoramidite methodology such as natural and modified DNA, RNA monomers as well as fluorophores and other modifications.

U29-E01. Automatic synthesizer
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U29-E02. High-performance liquid chromatography (HPLC) systems with a diode array detector

Analytical and preparative high-performance liquid chromatography (HPLC) system with a diode array detector.

U29-E02. High-performance liquid chromatography (HPLC) systems with a diode array detector.
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U29-E03. Speed-Vac evaporators

Speed-Vac evaporators for removal of water in oligonucleotide samples

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U29-E04. UV spectrophotometer equipped with a Peltier temperature gradient.

UV-Visible spectrophotometer equipped with a Peltier temperature gradient to run thermal denaturation assays followed by UV absorption.

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U29-E05. Spectrofluorimeter equipped with a Peltier temperature gradient.

Spectrofluorimeter equipped with a Peltier temperature gradient to run thermal denaturation assays and characterization of DNA-binding drugs followed by fluorescence.

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U28-E19. Beckman Coulter MoFlo cell sorter

Specifications:
Validated for 70,000 sorts per second with > 99% purity at all data rates
Proven 4-Way Sort for multiple population sorting
Independent control of each stream via touch screen control panel
High power solid state 488nm and 638nm lasers

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U28-E18. Beckman Coulter Galios flow cytometer

U28-E18. Beckman Coulter Galios flow cytometer

Specifications:
Solid State Diode Lasers: Blue 488nm (22mW), Red 638nm (25mW), Violet 405 (40mW)
10 independent fluorescence channels with easily interchangeable optical filters
Suitable for common fluorescence markers including: FITC, PE, ECD, PC5, PC5.5, PE-Cy7
125μm spatially separated beam spots
Gel coupled 1.2 NA optical collection lens
Forward scatter detector with adjustable collection angle for sub-micron, small and large particles sizes
Side scatter detector with independently focused high performance photodiode and electronic attenuation
Five different signals available from each detector: Integral linear and loga-rithmic, Peak linear and logarithmic and True Time of Flight linear Time, Ratio
User selectable flow rates (Low, Medium and High)
24-tube multi-loader carousel with integrated vortexer
Permits analysis of up to 88 tubes an hour at 10,000 events per second

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