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Equipments

Equipments

U9-E08. Laser Pyrolysis improvement

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U16-E07. Equipment for microbiology tests on materials. OUTSTANDING

Description: It is the set of precise elements to carry out microbiological tests with materials. Includes laminar flow adhesion chambers, Robbin devices, shaker and drip bioreactors, fluorescence microscopes, plate readers, ovens, autoclaves, etc.

Technical specifications: Allows quantifications on different types of systems. Depending on the specific conditions, it is possible to evaluate behavior in extensive solid materials or in suspension.

Applications: The services that can be provided with this equipment are:
Evaluation of the adhesion capacities and formation of biolayers of microorganisms on materials. Evaluation of bactericidal and/or bacteriostatic responses of materials.

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U13-E04. Micro-scale tension-compression test system CellScale.

Test system for small samples including:

  • Main equipment (Micro-scale tension-compression test system)
  • Tangential test module (Shear Axis add-on for MicroTester system)

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U30-E05. Small equipment for protein detection/quantification

Small equipment for protein detection/quantification

Description: Under this concept, the Unit has the following instruments:

  1. MagPix reader (Luminex), for the readout of magnetic bead–based immunoassays designed on xMAP beads. Allows the quantification of up to 50 analytes per sample.
  2. DIGE Imager (GE), for the digitalization of difference in-gel electrophoresis (DIGE) gels or stained with fluorescence (SYPRO, etc.).
  3. Amersham Imager 600 (GE), for the detection and digitalization of fluorescence or quimioluminiscence signals in gels or membranes.

Applications: Detection and/or quantification of proteins.

E05 MagPix reader
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U30-E04. Small equipment for protein separation

Small equipment for protein separation

Description: Under this concept, the Unit has the following instruments:

  1. High-performance liquid chromatography (HPLC) system with UV detector (Agilent 1200 Series), for the chromatographic separation of proteins by reversed phase, ion exange, gel filtration or affinity.
  2. Two IPGPhor systems (GE Healthcare), for the separation of proteins by isoelectric focusing.
  3. Protein electrophoresis units: MiniProtean (Bio-Rad), SE600 Ruby (GE Healthcare) y Ettan™ DALTsix (GE Healthcare), for running gels up to 24×26 cm to separate proteins according to their size.
  4. Microfraction collector and spotter SunCollect (SunChrom), to collect fractions coming out of the nLC and deposit themo onto MALDI plates for mass spectrometry. 

Applications: Separation of proteins in liquid or gel supports, according to a wide variety of physicochemical properties.

E04 SunChrom spotter
E04 HPLC 1200 series
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U30-E03. MALDI-TOF/TOF System

MALDI-TOF/TOF System

Description: 4800 Proteomics Analyzer (Sciex)

Technical specifications: Mass spectrometer equipped with a matrix-assisted laser desorption/ionization (MALDI) source, using a Nd:YAG 200-Hz laser, and two time-of-flight mass analyzers in tandem with a high-energy collision-induced disociation (CID) chamber for ion fragmentation. It has a variable ion path length to accomodate MS linear, MS reflector and MS/MS acquisition modes.

Applications: Determination of molecular masses of peptides, proteins and other biomolecules. Protein identification by peptide fingerprint or peptide fragmentation. Shotgun quantitative proteomics analyses by isotopic labeling and LC-MALDI workflows.

E03 MALDI-TOF/TOF System
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U30-E02. QTRAP LC-MS/MS System

QTRAP LC-MS/MS System

Description: Tempo™ nanoLC-2D liquid chromatography system coupled to QTRAP5500 mass spectrometer (Sciex).

Technical specifications: The QTRAP® 5500 LC-MS/MS system (Sciex) is a triple quadrupole combined with a trap-type accelerator. It has two electrospray ionization sources (nano source and turbo source).

Applications: Determination of molecular masses of peptides, proteins and other biomolecules. Absolute quantification of proteins using isotope-labeled standards. Targeted quantitative proteomics analyses by multiple reaction monitoring (MRM).

U30 E02 QTRAP LC-MS/MS System
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U30-E01. TIMS TOF Pro LC-MS/MS System

TIMS TOF Pro LC-MS/MS System

Description: Liquid nanochromatography system coupled to high resolution mass spectrometry with ion mobility (Bruker).

Technical specifications: The system consists of a nanoElute® chromatograph, which works at gradients between 50-2000 nL/min and is coupled to a TimsTOF mass spectrometer (Bruker) with an electrospray source. This spectrometer has two analyzers in hybrid configuration quadrupole and time of flight. It features a TIMS ion mobility trap that adds a fourth dimension of separation, as well as PASEF (Parallel Accumulation Serial Fragmentation) technology, which enables sequencing speeds of over 100 Hz, providing extremely high throughput and sensitivity.

Applications: Determination of molecular masses of peptides, proteins and other biomolecules. Protein identification by peptide fragmentation. Shotgun quantitative proteomics analyses by isotopic labeling (SILAC, iTRAQ, TMT…) or label-free (LFQ). Targeted quantitative proteomics analyses by PRM.

E01 TimsTOF Pro LC-MS/MS system (Bruker)
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U26-E07. MRS*DRYMAG3017-Flexiscan

Equipment Features:

Description: 3T preclinical imaging equipment with PET ClipOn attached.

Technical specifications: Imaging equipment with superconducting magnet, capable of varying the angle from 0 to 90º, without cryogenics of 3 Teslas; mouth diameter of 170mm; 600 mT/m gradients with 5% linearity; 30 mm DSV for Homogeneity and 0.05 ppm/hour for stability; 350 Kg of total weight.

Applications:

Acquisition and viewing of in vivo images of mice and rats as well as other beings or materials of similar size.

  • Qualitative and quantitative studies (relaxation times T1 and T2 and even making maps) at the brain level and other organs.
  • Cardiological sequences: diffusion, determination of blood volume…
    Anatomical and functional studies of different organs (for example: renal, abdominal, liver…).
  • Bone-level imaging, distinguish between bone material from soft tissue.
    Fat suppression techniques.
  • Oncological studies to detect and locate tumors and volume calculation.
  • Carry out studies on fruits and vegetables (grapes, kiwi, tomato, seeds…).
  • Perform studies with/without contrast (Gadolinium) and see the differences between the 2 images.
  • Thanks to the possibility of rotating the equipment in a vertical position, images of plants can be obtained (because by their nature, they have to be in a vertical orientation, such as a stem).
  • Ex-vivo studies can also be carried out vertically and in this way we maintain the integrity of the organ (eg heart).
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U8-E15. Dual extrusion 3D printer (Ultimaker S3, Ultimaker BV)

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