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Equipments

Equipments

U29-E06. UV-Visible and luminometer Reader, multidetection system.

UV-Visible and luminometer Reader used in luciferase and MTT assays

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U29-E07. Electrophoresis apparatus with several vertical and horizontal cuvettes.

Electrophoresis apparatus with several vertical and horizontal cuvettes for agarose and polyacrylamide (PAGE) gel electrophoresis.

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U29-E01. Automatic synthesizer.

Automatic synthesizer which can be operated at a range of scales (40, 200, 1000 nanomols). A large variety of monomers can be incorporated using the phosphoramidite methodology such as natural and modified DNA, RNA monomers as well as fluorophores and other modifications.

U29-E01. Automatic synthesizer
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U29-E02. High-performance liquid chromatography (HPLC) systems with a diode array detector

Analytical and preparative high-performance liquid chromatography (HPLC) system with a diode array detector.

U29-E02. High-performance liquid chromatography (HPLC) systems with a diode array detector.
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U29-E03. Speed-Vac evaporators

Speed-Vac evaporators for removal of water in oligonucleotide samples

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U29-E04. UV spectrophotometer equipped with a Peltier temperature gradient.

UV-Visible spectrophotometer equipped with a Peltier temperature gradient to run thermal denaturation assays followed by UV absorption.

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U29-E05. Spectrofluorimeter equipped with a Peltier temperature gradient.

Spectrofluorimeter equipped with a Peltier temperature gradient to run thermal denaturation assays and characterization of DNA-binding drugs followed by fluorescence.

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U28-E19. Beckman Coulter MoFlo cell sorter

Specifications:
Validated for 70,000 sorts per second with > 99% purity at all data rates
Proven 4-Way Sort for multiple population sorting
Independent control of each stream via touch screen control panel
High power solid state 488nm and 638nm lasers

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U28-E18. Beckman Coulter Galios flow cytometer

U28-E18. Beckman Coulter Galios flow cytometer

Specifications:
Solid State Diode Lasers: Blue 488nm (22mW), Red 638nm (25mW), Violet 405 (40mW)
10 independent fluorescence channels with easily interchangeable optical filters
Suitable for common fluorescence markers including: FITC, PE, ECD, PC5, PC5.5, PE-Cy7
125μm spatially separated beam spots
Gel coupled 1.2 NA optical collection lens
Forward scatter detector with adjustable collection angle for sub-micron, small and large particles sizes
Side scatter detector with independently focused high performance photodiode and electronic attenuation
Five different signals available from each detector: Integral linear and loga-rithmic, Peak linear and logarithmic and True Time of Flight linear Time, Ratio
User selectable flow rates (Low, Medium and High)
24-tube multi-loader carousel with integrated vortexer
Permits analysis of up to 88 tubes an hour at 10,000 events per second

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U28-E17. Perkin Elmer Operetta HCS confocal

U28-E17. Perkin Elmer Operetta HCS confocal

Specifications:
Spinning disc confocal technology
10x, 20x and 40x long working distance objectives
20x high NA objectives
Temperature control and CO2 for cultured cells
à 400W Xenon based illumination
Fully automated image acquisition from 24,96 and 384 well plates
Harmony: integrated acquisition and analysis software
Phenologic – machine learning-based phenotype recognition software
Columbus Image Data Storage and Analysis System

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