Description: It is the set of precise elements to carry out microbiological tests with materials. Includes laminar flow adhesion chambers, Robbin devices, shaker and drip bioreactors, fluorescence microscopes, plate readers, ovens, autoclaves, etc.
Technical specifications: Allows quantifications on different types of systems. Depending on the specific conditions, it is possible to evaluate behavior in extensive solid materials or in suspension.
Applications: The services that can be provided with this equipment are:
Evaluation of the adhesion capacities and formation of biolayers of microorganisms on materials. Evaluation of bactericidal and/or bacteriostatic responses of materials.
Test system for small samples including:
Small equipment for protein detection/quantification
Description: Under this concept, the Unit has the following instruments:
Applications: Detection and/or quantification of proteins.
Small equipment for protein separation
Description: Under this concept, the Unit has the following instruments:
Applications: Separation of proteins in liquid or gel supports, according to a wide variety of physicochemical properties.
MALDI-TOF/TOF System
Description: 4800 Proteomics Analyzer (Sciex)
Technical specifications: Mass spectrometer equipped with a matrix-assisted laser desorption/ionization (MALDI) source, using a Nd:YAG 200-Hz laser, and two time-of-flight mass analyzers in tandem with a high-energy collision-induced disociation (CID) chamber for ion fragmentation. It has a variable ion path length to accomodate MS linear, MS reflector and MS/MS acquisition modes.
Applications: Determination of molecular masses of peptides, proteins and other biomolecules. Protein identification by peptide fingerprint or peptide fragmentation. Shotgun quantitative proteomics analyses by isotopic labeling and LC-MALDI workflows.
QTRAP LC-MS/MS System
Description: Tempo™ nanoLC-2D liquid chromatography system coupled to QTRAP5500 mass spectrometer (Sciex).
Technical specifications: The QTRAP® 5500 LC-MS/MS system (Sciex) is a triple quadrupole combined with a trap-type accelerator. It has two electrospray ionization sources (nano source and turbo source).
Applications: Determination of molecular masses of peptides, proteins and other biomolecules. Absolute quantification of proteins using isotope-labeled standards. Targeted quantitative proteomics analyses by multiple reaction monitoring (MRM).
TIMS TOF Pro LC-MS/MS System
Description: Liquid nanochromatography system coupled to high resolution mass spectrometry with ion mobility (Bruker).
Technical specifications: The system consists of a nanoElute® chromatograph, which works at gradients between 50-2000 nL/min and is coupled to a TimsTOF mass spectrometer (Bruker) with an electrospray source. This spectrometer has two analyzers in hybrid configuration quadrupole and time of flight. It features a TIMS ion mobility trap that adds a fourth dimension of separation, as well as PASEF (Parallel Accumulation Serial Fragmentation) technology, which enables sequencing speeds of over 100 Hz, providing extremely high throughput and sensitivity.
Applications: Determination of molecular masses of peptides, proteins and other biomolecules. Protein identification by peptide fragmentation. Shotgun quantitative proteomics analyses by isotopic labeling (SILAC, iTRAQ, TMT…) or label-free (LFQ). Targeted quantitative proteomics analyses by PRM.
Equipment Features:
Description: 3T preclinical imaging equipment with PET ClipOn attached.
Technical specifications: Imaging equipment with superconducting magnet, capable of varying the angle from 0 to 90º, without cryogenics of 3 Teslas; mouth diameter of 170mm; 600 mT/m gradients with 5% linearity; 30 mm DSV for Homogeneity and 0.05 ppm/hour for stability; 350 Kg of total weight.
Applications:
Acquisition and viewing of in vivo images of mice and rats as well as other beings or materials of similar size.