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News U2

News U2

Workshop on “ETHICAL ASPECTS OF RESEARCH”

The Nanobiotechnology for Diagnostics group (Nb4D) of CIBER-BBN and IQAC-CSIC, (coordinator of NANBIOSIS U2 Custom Antibody Service (CAbS)), led by Prof. Mª Pilar Marco, is organizing a workshop on “Ethical aspects of research” netx 18th November 2019, at the Institute for Advanced Chemistry of Catalonia (IQAC-CSIC). 

There will be three sessions given by experts from this topic area.

  • TECHNOLOGICAL ADVANCES VERSUS ETHICS by Dr. José Ignacio Latorre
  • ANIMAL TESTING by Dr. Lluís Montoliu
  • CLINICAL SAMPLES, BIOBANKS AND OTHER ASPECTS IN CLINICAL PRACTICE by Dr. Pieter Moons

Certificate of Attendance will be issued for each participant.

This workshop is FREE but has LIMITED SPACE. To  register, fill in the form by 10th November 2019https://bit.ly/2VI5W3j

Venue: Salón de Actos, CID-CSIC C/Jordi Girona 18-36. Barcelona. Monday, November 18, 2019, 9:30 h

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Micro & nano(bio)technology for Diagnosis: Changes and Challenges

Pilar Marco, Scientific Director of NANBIOSIS U2 Custom Antibody Service (CAbS) participated as Keynote speakers at the Second European Biosensor Symposium (EBS2019) taking about “Micro & nano(bio)technology for Diagnosis: Changes and Challenges” at the 2nd European Biosensor Symposium 2019 that took place in Florence (Italy) Last February 18-21.

The EBS brougth toguether young scientists, experienced researchersand companies to push forward new approaches on biosensor and and their applications,

Pilar Marco spoke abaut the changes ocurred on the field of Diagnosis since the days of “house calls”, relying primarily on physical examination, to nowadays in which a wide portfolio of biochemical analytical tests is available, and pointed out the challenge to improve knowledge in microfluidics, lab-on-a-chip technologies, system integration, device automation, and signal readout, as well as simultaneously measure distinct sets of biomolecular features on a reproducible and reliable manner. Prof. Marco also gave some examples of technological alternatives to circumvent these limitations in the clinical diagnostic field.

Pictures by Rudolf Schneider https://bit.ly/2T0yYNi

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PILAR MARCO, Scientific Director of NANBIOSIS U2, interviewed by CIBER-BBN ISCIII Bulletin

Pilar Marco, Scientific Director of NANBIOSIS U2 Custom Antibody Service (CAbS) has been highlighted in the January Bulletin of CIBER-BBN ISCIII

In the interview, among other topics Prof. Pilar Marco talks about diagnostic devices based on nanobiosensors and the extraordinary impact that these technologies could have on health in the coming decades.

Her research group Nb4D (Nanobiotechnology for Diagnosis) has an important collection of specific antibodies for different biomarkers and the CIBER-BNN/IQAC-CSIS platform CAbS (Custom Antibody Service) that constitutes the unit 2 of NANBIOSIS, they offer the possibility of producing specific and immunoreactive antibodies with the necessary expertise to generate antibodies “a la carte”, that is, being able to modulate to some extent the affinity and selectivity of these biomolecules, depending on the needs of each project. “And we do this, -Prof Marco explains- not only for molecules with high immunogenic capacity such as most proteins, but also for low molecular weight molecules, which in themselves are not capable of generating an immune response”. They are also able to chemically modify these antibodies and bind them to nanoparticles with defined optical, electrochemical or magnetic properties, thus converting them into nanoprobes able to detect biomarkers and generate an optical or electrochemical signal. We can also incorporate them in a controlled manner in transducer devices designed based on the latest advances in micro (nano) electronics to develop a new generation of diagnostic devices, much more sensitive and reliable, capable of providing quick answers and allowing a more accurate diagnosis early and accurate.

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NANBIOSIS Scientific Women in the International Day of Women and Girls in Science

Today February 11 is the International Day of Women and Girls in Science, a day to raise awareness of the gender gap in science and technology.

According to the United Nations, while yet women and girls continue to be excluded from participating fully in science, science and gender equality are vital to achieve the internationally agreed development goals, including the 2030 Agenda for Sustainable Development. Thus, in recent years, the international community has made a great effort to inspire and promote the participation of women and girls in science.

NANBIOSIS wants to acknowledge  the efforts made by scientific women who struggle every day to contribute their bit to Science and highlight their essential role in nowadays research. Especially we want to recognize the work of scientists women involved in our units, whatever is the nature of their contribution: technical, scientific development, management, coordination, direction, etc; just to mention some examples:
Neus Ferrer in the Scientific Direction of Unit 1 Protein Production Platform (PPP)
Pilar Marco and Nuria Pascual in the Management and Scientific Coordination of U2 Custom Antibody Service (CAbS) 
Miriam Royo in the Scientific Direction of U3 Synthesis of Peptides Unit
Laura Lechuga and M.Carmen Estevez in the Direction and Scientific Coordination of U4 Biodeposition and Biodetection Unit
Nora Ventosa and Nathaly Segovia in the Scientific Direction and Technical Coordination of U6 Biomaterial Processing and Nanostructuring Unit
Isabel Oliveira and Teresa Galán in the Coordination of U7 Nanotecnology Unit
Rosa Villa and Gemma Gabriel in the Management and Scientific Coordination of U8 Micro – Nano Technology Unit
Gema Martínez in the Scientific Coordination of U9 Synthesis of Nanoparticles Unit
Fany Peña in the Scientific Coordination of U13 Tissue & Scaffold Characterization Unit
Mª Luisa González Martín in the of Direction and Scientific Coordination of U16 Tissue & Scaffold Characterization Unit
Gemma Pascual and Isabel Trabado in the Coordination of the U17 Confocal Microscopy Service
Mª Virtudes Céspedes in the Scientific Coordination of U18 Nanotoxicology Unit
Beatriz Moreno in the Scientific Direction of Unit 19 Clinical tests lab
Ibane Abásolo in the Scientific Coordination of Unit 20 In Vivo Experimental Platformt
Verónica Crisóstomo in the Scientific Direction of Unit 24 Medical Imaging 

Ana Paula Candiota in the Scientific Coordination of Unit 25 Biomedical Applications I 
Maria Luisa García in the Scientific Direction of U28 NanoImaging Unit from Bionand, recently incorporated to NANBIOSIS

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New device could monitor treatments with anticoagulant to achive personalized therapies

  • Researchers from NANBIOSIS units 2 and 4 have designed a biosensor device based on gold nanostructures that precisely detects the presence of the anticoagulant drug from a small blood sample.
  • Treatments with anticoagulants such as Sintrom® (acenocoumarol) face the difficulty of adjusting the drug doses. This device will allow patients to self-regulate the dose.

 

Researchers of NANBIOSIS unit 4  Biodeposition and Biodetection Unit of the Consejo Superior de Investigaciones Científicas (CSIC), the Catalan Institute of Nanoscience and Nanotechnology (ICN2) and CIBER Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) have developed a biosensor device which allows the monitoring of anticoagulants such as Sintrom® (acenocoumarol) to deliver a personalized therapy in which the patient or doctor can adjust the drug dose to achieve the optimal effect.

The ICN2 Nanobiosensors and Bioanalytical Applications (NanoB2A) Group, led by CSIC researcher Prof. Laura Lechuga, Scientific Director of NANBIOSIS U4 (Biodeposition and Biodetection Unit), designed this plasmonic device containing gold nanostructures to which specific bioreceptors can be attached, in order to detect biomarkers in a reproducible and accurate way using a small sample of the patient’s blood and without the need for any prior treatment.

Previously, the Nanobiotechnology for Diagnostics (Nb4D) Group of the CSIC’s Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), led by Prof. M. Pilar Marco, Scientific Director of NANBIOSIS U2 (Custom Antibody Service (CAbS),  produced haptenized proteins (similar to Sintrom®) and polyclonal antibodies specific for this anticoagulant in the U2 of NANBIOSIS. The large experience of U2 unit in the preparation of labeled proteins and production of monoclonal and polyclonal antibodies has resulted in the generation of specific antibodies capable of recognizing Sintrom®. Their usefulness has been demonstrated in a clinical study using an immunoanalytical technique with high sample throughput.

Building on their previous results, the two groups collaborated to develop an automated biosensor device allowing a personalized dose adjustment of this oral anticoagulant. Their results were recently published in Biosensors and Bioelectronics.

As stated by CSIC researcher Prof. Laura Lechuga, “we have studied the optimal conditions to develop this biosensor device and have obtained excellent sensitivity to detect the anticoagulant, which indicates that it is possible to measure very low concentrations of this drug”.

Since it is a quantitative and highly sensitive technology, this device is very suitable for its use in clinical environments, where it could provide a real time follow-up of the medication, or even for patients to manage the medication themselves, a significant breakthrough for people receiving anticoagulants.

Patients suffering from cardiovascular diseases or thromboembolic disorders are usually treated with anticoagulants such as Sintrom® to prevent blood clots formation. The problem is that this treatment entails risks, explain the researchers. “For example, in case the dose is too low, it would not achieve the expected effect and blood clotting may occur; On the other hand, in case the dose is too high, it could cause side effects such as internal bleeding. The right dose depends on many factors such as weight, age, diet and interaction with other medications. ”

 

Reference articles:

Nanoplasmonic biosensor device for the monitoring of acenocoumarol therapeutic drug in plasma Cristina Peláez, M Carmen Estévez, Alejandro Portela, Pablo Salvador, Pilar Marco, Laura M. Lechuga. Biosensors and Bioelectronics, 119, 2018, 149-155 – DOI: 10.1016/j.bios.2018.08.011

Enzyme-linked immunosorbent assays for therapeutic drug monitoring coumarin oral anticoagulants in plasma J.Pablo Salvador, Dolors Tassies, Joan-Carles Reverter, M.-PilarMarco. Analytica Chimica Acta. https://doi.org/10.1016/j.aca.2018.04.042

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Two units of NANBIOSIS in the project selected by La Marató TV3 to fight against bacterial infections

La Marató TV3 in its call for biomedical projects in infectious diseases has selected the project “Uses of molecular analysis of Quorum Sensing with the diagnosis of bacterial infections” of the Nb4D group, led by Mª Pilar Marco, Scientific Director of unit 2 of NANBIOSIS and in which Miriam Royo also participates Scientific Coordinator of unit 3 of NANBIOSIS, both in the IQAC-CSIC of Barcelona

The project of the Nanobiotechnology for Diagnosis (Nb4D) group of the CSIC and the CIBER-BBN has been selected among more than 200 candidates as a project of scientific excellence to be funded in the call for biomedical projects of the Fundació la Marató de TV3 and Catalonia Radio. The project, led in the Nb4D group by Dr. M.-Pilar Marco, Scientific Director of unit 2 of NANBIOSIS and Dr. Lluïsa Vilaplana, will be carried out in collaboration with Dr. M.-Teresa Martín (Hospital Vall d’Hebrón) and aims to study the potential of various molecules of Quorum Sensing as diagnostic tools for bacterial infections. The project, financed with € 227,684.84, also has the collaboration of Dr. Miriam Royo, Scientific Coordinator of Unit 3 of NANBIOSIS, of the Multivalent Systems for Nanomedicine group, also belonging to the CSIC and the CIBER-BBN.

The funds raised in the 2017 edition of the Marató de TV3 and Cataluña Radio will finance 36 biomedical research projects of excellence in infectious diseases to promote the creation of new tools for prevention and diagnosis, as well as more efficient treatments with the aim of earning more and more quality of life for patients.

Link to the news:
http://www.ccma.cat/324/la-marato-impulsa-36-projectes-de-recerca-biomedica-en-malalties-infeccioses/noticia/2884103/

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NANBIOSIS in the Posters presentation in CIBER-BBN ANNUAL CONFERENCE 2018

Last 12 and 13 of November, CIBER-BBN  has celebrated its 12th Annual Conference in Hotel Felipe IV in Valladolid. In poster session participated  NANBIOSIS  itself and some of its units.

  • NANBIOSIS, Infrastructure for the Production & Characterization of Biomaterials, Nanomaterials and medical devices up to preclinical validation. Nanbiosis management team.
  • Divalent cation effects on assembly of histidine-rich protein nanoparticles. López-Laguna, U. Unzueta, O. Conchillo-Solé, A. Sánchez-Chardi, M. Pesarrodona, O. Cano-Garrido, E. Voltà, L. Sánchez-García, N. Serna, P. Saccardo, R. Mangues, A. Villaverde, E. Vázquez (NBT-UAB) (U1 -U18)
  • Antimicrobial peptides (AMPs) anchored on the surface of contact lenses to prevent corneal infections. Emiliano Salvagni, Clara García, Àngels Manresa, Carlos Rodríguez, María José García-Celma, Claudia Müller-Sánchez, Manuel Reina, Jordi Esquena (QCI-CSIC) (U12)
  • Preliminary studies on ultrasound characterization of perfluorocarbon-loaded polymeric nanocapsules. Gabriela Calderó, Marie Pierre Krafft, Da Shi, María José García-Celma, Conxita Solans, Carlos Rodríguez-Abreu (QCI-CSIC) (U12)
  • Multiplexed analytical platforms based on the use of antibodies for monitoring pollutants in marine environment samples. -Pablo Salvador, Ana Sanchís, Klaudia Kooper, Andrea Miti, M.-Pilar Marco (Nb4D-IQAC-CSIC) (U2)
  • In vitrodiagnostics of neurological disorders through the immunochemical detection of kynurenine. Montserrat Rodríguez Núñez, Ana Sanchís, Lluïsa Vilaplana, Roger Galve, M.-Pilar Marco (Nb4D-IQAC-CSIC) (U2)
  • Custom Antibody Service: From the molecule to the bioassay. Núria Pascual, Ana González-Gomzález, M.-Pilar Marco (Nb4D-IQAC-CSIC) (U2)
  • Hyaluronic acid enhances insulin release of microencapsulated pancreatic progenitors differentiated from mesenchymal stem cells. Alberto Cañibano-Hernández, Laura Sáenz del Burgo, Albert Espona-Noguera, Gorka Orive, Rosa María Hernández, Jesús Ciriza, Jose Luis Pedraz (NANOBIOCEL) (U10 )
  • Type 1 Diabetes Mellitus reversal via implantation of magnetically purified microencapsulated pseudoislets. Albert Espona-Noguera, J. Etxebarria-Elezgarai, L. Saenz del Burgo, A. Cañibano-Hernández, H. Gurruchaga, Gorka Orive, Rosa M. Hernández, F. Benito-Lopez, J. Ciriza, L. Basabe-Desmonts and J.L. Pedraz (NANOBIOCEL) (U10 )
  • Unraveling the immune system participation in therapy response in GL261 glioblastoma: correlation with MRSI-based molecular imaging techniques. Calero, N. Arias-Ramos, R. Rabanal, M. Pumarola, C. Arús, A.P. Candiota (GABRMN-UAB) (U25)
  • Dual T1/T2 nanoscale coordination polymers as novel contrast agents for MRI: a preclinical study for brain tumor. Suárez-García, N. Arias-Ramos, C. Frias, A.P. Candiota, C. Arús, J. Lorenzo, D. Ruiz-Molina, F. Novio (GABRMN-UAB) (U25)
  • Impact of high glucose levels and ketoacidosis associated to diabetic complications on bacterial growth and attachment to Ti6Al4V. Miguel A. Pacha-Olivenza, María Fernández-Grajera, Daniel Romero-Guzmán, M. Luisa González-Martín (AM-UEX) (U16)
  • Preparation, characterization and bacterial performance on PLDA and PLDA 10% Mg films. Verónica Luque-Agudo, Daniel Romero-Guzmán, Coronada Fernández-Calderón, Miguel A. Pacha-Olivenza, María Fernández-Grajera, Margarita Hierro-Oliva, M. Luisa Navarro-Pérez, Ciro Pérez-Giraldo, M. Luisa González-Martín, Amparo M. Gallardo-Moreno (AM-UEX) (U16)
  • A wavelet-based approach for automatic diagnosis of strict left bundle branch block. Alba Martín-Yebra, Juan Pablo Martínez (BSICoS) (U27)
  • ECG-derived respiration in atrial fibrillation. Spyridon Kontaxis, Jesús Lázaro, Valentina D. A. Corino, Frida Sandberg, Raquel Bailón, Pablo Laguna, and Leif Sörnmo (BSICoS) (U27)
  • Development of highly metastatic, CXCR4-overexpressing, colorectal cancer models. Rita Sala, Alberto Gallardo, Ugutz Unzueta, Patricia Álamo, Carmen Cabrera, Esperanza Medina, Isolda Casanova, Irene Arroyo, Aïda Falgás, Carlos Navas, Manuel Trías, Antonio Villaverde, Esther Vázquez, Ramón Mangues, Mª Virtudes Céspedes (GOA-HSPau) (U18)
  • PATHGATE: Oligonucleotide-gated sensing nanodevices for pathogen detection. Luis Pla, Angela Ribes, M. Angeles Tormo-Mas, Javier Pemán, Félix Sancenón, Elena Aznar, Ramón Martínez-Máñez and Sara Santiago-Felipe (IQMA-IDM-UPV) (U26)
  • Towards chemical communication between abiotic nanoparticles and living systems. Beatriz de Luis, Paola Ricón, Cristina de la Torre, Antoni LLopis, Jose Gadea, Jose R. Murguía, Félix Sancenón, Ramón Martínez-Máñez, Elena Aznar (IQMA-IDM-UPV) (U26)
  • Development  and  in vitro evaluation  of  antimicrobial  polymer  coatings  for the prophylactic treatment of hernia repair materials. Bárbara Pérez-Köhler, Selma Benito, Mar Fernández-Gutiérrez, Gemma Pascual, Marta Rodríguez, Verónica Gómez-Gil, Francisca García-Moreno, Julio San Román, Juan Manuel Bellón (GITBIT-UAH) (U17)
  • Experimental study of the application of a new bone cement loaded with broad spectrum antibiotics for the treatment of bone infection. Joaquín García, Galo Azuara, Blanca Ibarra, Miguel A. Ortega, Ángel Asúnsolo, Blanca Vázquez, Julio San Román, Julia Buján, Basilio De la Torre, Natalio García-Honduvilla (GITBIT-UAH) (U17)
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Nanoplasmonic biosensor device for the monitoring of acenocoumarol therapeutic drug in plasma

As a result of the collaboration carried aout by two units of NANBIOSIS, U4. Biodeposition and Biodetection Unit and U2. Custom Antibody Service (CAbS), it has been developed a compact and simple nanoplasmonic sensing device based on gold nanodisks for the rapid monitoring of acenocoumarol, using highly specific polyclonal antibodies produced against the drug Acenocoumarol (Sintrom®) which implies:

  • A label-free nanoplasmonic device for the rapid monitoring of acenocoumarol in plasmaD.
  • Direct quantification in real time requiring low sample volume is achieved.
  • Non-specific interferences from plasma are minimized using the developed methodology.
  • Excellent accuracy has been observed measuring blind plasma samples.
  • Potential to be implemented as a POC device in decentralized settings.

 

The research details have been published in the article authored by E. CristinaPeláezM.-CarmenEstevezAlejandroPortelaJ.-PabloSalvadorM.-PilarMarco and Laura M.Lechuga, “Nanoplasmonic biosensor device for the monitoring of acenocoumarol therapeutic drug in plasma, Biosensors and Bioelectronic, 119, 2018, 149-155 – DOI: https://doi.org/10.1016/j.bios.2018.08.011

 

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Interferometric nanoimmunosensor for label-free and real-time monitoring of Irgarol 1051 in seawater

As a result of a collaboration in the context of the EC-FP7 program OCEAN 2013, NANBIOSIS Unit 2 Custom Antibody Service (CAbS) and  Unit 4. Biodeposition and Biodetection,  the research groups coordinating this NANBIOSIS Units have recently published an article titled “Interferometric nanoimmunosensor for label-free and real-time monitoring of Irgarol 1051 in seawater” in the scientific magzine  iosensors and Bioelectronics.

The CIBER-BBN-ICN2 group  Nanobiosensors and Bioanalytical Applications, led by Prof. Laura Lechuga has developed the immunosensor that is described in the article using immunoreactive, produced in the group of  Nanobiotechnology for Diagnostics Group (Nb4D), led by Prof. Pilar Marco, with the collaboration of CAbS, for Irgarol 1051. This compound is an alguicide used as an additive in the paintings of ships. Over time, the compose is being released  into the marine environment causing a risk to the ecosystem and to the health of the population.

In the article, an interferometric nanobiosensor for the specific and label-free detection of the pollutant Irgarol 1051 directly in seawater has been settled. Due to the low molecular weight of Irgarol pollutant and its expected low concentration in seawater, the sensor is based on a competitive inhibition immunoassay. Parameters as surface biofunctionalization, concentration of the selective antibody and regeneration conditions have been carefully evaluated. The optimized immunosensor shows a limit of detection of only 3 ng/L, well below the 16 ng/L set by the EU as the maximum allowable concentration in seawater. It can properly operate during 30 assay-regeneration cycles using the same sensor biosurface and with a time-to-result of only 20 min for each cycle. Moreover, the interferometric nanosensor is able to directly detect low concentrations of Irgarol 1051 in seawater without requiring sample pre-treatments and without showing any background signal due to sea matrix effect.

Article of referencehttps://doi.org/10.1016/j.bios.2018.05.044

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NANBIOSIS U2 incorporates a new Nitrogen tank to store cells

NANBIOSIS U2. Custom Antibody Service (CAbS), is incorporating new equipment as a result of its participation in the project FICTS1420-27, selected by the MINECO for co-financing by the FEDER Program in ICTS 2014-2020.

Recently the purchase and installation and set-up of a new nitrogen tank to store cells has taken place. The storage of cell lines of monoclonal antibody-producing hybridomas is a process in which the cells are conserved when they are cooled to temperatures typically at -196 ° C in liquid Nitrogen tanks. With temperatures below -130 ° C the activity Biological ceases and storage at these temperatures is vital to keep the material unaltered. The increasing activity of the NANBIOSIS U2 the makes necesary to incorporate a new tank of liquid Nitrogen for the storage of new hybridoma lines.

For further information:

 

Liquid nitrogen tank
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