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Preclinical validation characterization - Equipment

Preclinical validation characterization – Equipment

U30-E05. Small equipment for protein detection/quantification

Small equipment for protein detection/quantification

Description: Under this concept, the Unit has the following instruments:

  1. MagPix reader (Luminex), for the readout of magnetic bead–based immunoassays designed on xMAP beads. Allows the quantification of up to 50 analytes per sample.
  2. DIGE Imager (GE), for the digitalization of difference in-gel electrophoresis (DIGE) gels or stained with fluorescence (SYPRO, etc.).
  3. Amersham Imager 600 (GE), for the detection and digitalization of fluorescence or quimioluminiscence signals in gels or membranes.

Applications: Detection and/or quantification of proteins.

E05 MagPix reader
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U30-E04. Small equipment for protein separation

Small equipment for protein separation

Description: Under this concept, the Unit has the following instruments:

  1. High-performance liquid chromatography (HPLC) system with UV detector (Agilent 1200 Series), for the chromatographic separation of proteins by reversed phase, ion exange, gel filtration or affinity.
  2. Two IPGPhor systems (GE Healthcare), for the separation of proteins by isoelectric focusing.
  3. Protein electrophoresis units: MiniProtean (Bio-Rad), SE600 Ruby (GE Healthcare) y Ettan™ DALTsix (GE Healthcare), for running gels up to 24×26 cm to separate proteins according to their size.
  4. Microfraction collector and spotter SunCollect (SunChrom), to collect fractions coming out of the nLC and deposit themo onto MALDI plates for mass spectrometry. 

Applications: Separation of proteins in liquid or gel supports, according to a wide variety of physicochemical properties.

E04 SunChrom spotter
E04 HPLC 1200 series
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U30-E03. MALDI-TOF/TOF System

MALDI-TOF/TOF System

Description: 4800 Proteomics Analyzer (Sciex)

Technical specifications: Mass spectrometer equipped with a matrix-assisted laser desorption/ionization (MALDI) source, using a Nd:YAG 200-Hz laser, and two time-of-flight mass analyzers in tandem with a high-energy collision-induced disociation (CID) chamber for ion fragmentation. It has a variable ion path length to accomodate MS linear, MS reflector and MS/MS acquisition modes.

Applications: Determination of molecular masses of peptides, proteins and other biomolecules. Protein identification by peptide fingerprint or peptide fragmentation. Shotgun quantitative proteomics analyses by isotopic labeling and LC-MALDI workflows.

E03 MALDI-TOF/TOF System
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U30-E02. QTRAP LC-MS/MS System

QTRAP LC-MS/MS System

Description: Tempo™ nanoLC-2D liquid chromatography system coupled to QTRAP5500 mass spectrometer (Sciex).

Technical specifications: The QTRAP® 5500 LC-MS/MS system (Sciex) is a triple quadrupole combined with a trap-type accelerator. It has two electrospray ionization sources (nano source and turbo source).

Applications: Determination of molecular masses of peptides, proteins and other biomolecules. Absolute quantification of proteins using isotope-labeled standards. Targeted quantitative proteomics analyses by multiple reaction monitoring (MRM).

U30 E02 QTRAP LC-MS/MS System
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U30-E01. TIMS TOF Pro LC-MS/MS System

TIMS TOF Pro LC-MS/MS System

Description: Liquid nanochromatography system coupled to high resolution mass spectrometry with ion mobility (Bruker).

Technical specifications: The system consists of a nanoElute® chromatograph, which works at gradients between 50-2000 nL/min and is coupled to a TimsTOF mass spectrometer (Bruker) with an electrospray source. This spectrometer has two analyzers in hybrid configuration quadrupole and time of flight. It features a TIMS ion mobility trap that adds a fourth dimension of separation, as well as PASEF (Parallel Accumulation Serial Fragmentation) technology, which enables sequencing speeds of over 100 Hz, providing extremely high throughput and sensitivity.

Applications: Determination of molecular masses of peptides, proteins and other biomolecules. Protein identification by peptide fragmentation. Shotgun quantitative proteomics analyses by isotopic labeling (SILAC, iTRAQ, TMT…) or label-free (LFQ). Targeted quantitative proteomics analyses by PRM.

E01 TimsTOF Pro LC-MS/MS system (Bruker)
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U16-E06. 3D Optical Surface Metrology System Leica DCM8 (Remote) OUTSTANDING

FICTS-1420-14-09 3. Optical profilometer

3D Optical Surface Metrology System Leica DCM8

This equipment allows measuring the roughness of a multitude of samples by means of a non-destructive optical method. Based on the operation of a confocal microscope, it allows the creation of high-resolution 3D images in a fast and automatic way as well as obtaining color images thanks to the use of three LEDs: Red, Green and Blue. In addition, this system also works as an interferometer that allows to measure the roughness with greater precision of mirror polished samples.

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U16-E05. DROP SHAPE ANALYZER – DSA100E (Remote) OUTSTANDING

FICTS-1420-14-09 4. Goniometer

DROP SHAPE ANALYZER – DSA100E

The system measures the contact angle between a liquid and a solid. Offers the possibility of measuring static, advancing and receding contact angle, as well as contact angle hysteresis by a tilting table. Also, measurement of surface tension and liquid-liquid interfacial tension using the Pendant Drop method can be obtained. Thanks to tempering and humidity chambers, this equipment can realistically reproduce individual process conditions, by making measurements under controlled humidity and temperature from 5 to 90 °C.

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U12-E22. SEM Hitachi TM4000Plus II for morphological and dimensional characterization of solid samples

U12-E22. SEM Hitachi TM4000Plus II for morphological and dimensional characterization of solid samples

Description:

Low vacuum Scanning Electron Microscopy with two electron detectors (secondary, SE, and backscattered, BSE). SE imaging provides a detailed information of the morphology of the sample and BSE gives information about the different components of the sample, based on the different atomic number (the higher the atomic number, the brighter the surface).

Appropriate vacuum levels are reached using a turbo molecular pump and electron beams are directed to sample specimens with accelerating voltages between 5 and 20 kV, which allow magnifications from 10x to 100,000x. Specimens with size up to 80 mm diameter and/or 50 mm thickness can be observed.

Conductive and non-conductive samples can be characterized with little or no previous preparation. The software enables image processing of micrographs, for measuring the size of solid particles after the acquisition of images.

Applications:

Topographical and morphological characterization of tissues, hairs, fibers, etc. Quality control and failure analysis. Morphological properties are quite important in the research of innovative materials. In addition, the characterization of the surface is a key factor in porous materials used for delivery systems. Semiconductor inspection and microchip assembly, to analyse the topography and to investigate the effectiveness of production methods.

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U19-E02. Specific laboratory diagnostic equipment: Hematology, biochemistry, hormones, biomarkers analyzer

Specific laboratory diagnostic equipment: Hematology, biochemistry, hormones, biomarkers analyzer:

  • Automatic coagulometer (CLOT SP de RAL)
  • Biochemical Analysis (VetScan)
  • Solid state thermostatic equipment 37 º C + – 0,1 º C with magnetic stirrer system, 15 reaction cubiletes and a reacive bottle
  • Automatic coagulometer (AUTOCLOT SP de RAL)
  • Clinic auto-analyser (Metrolab 2300 de RAL)
  • Blood cells counter (MEK-6318. NIHON KOHDEN)
  • AQT90 FLEX analyzer (RADIOMETER)
  • Water purification system (Elix 3 de MILLIPORE)
  • Water purification system (Milli-Q Advantage A10 de MILLIPORE)
  • Laboratory freezer (MEDILOW-M SELECTA)
  • Vertical Freezer (SF-126) from -30 ºC to 150 L capacity
  • Digital automatic micropipettes
  • Centrifuge SELECTA (MIXTASEL 7000575)
  • Rotary shaker (NAHITA)
  • Recorder Device leukocyte (Leucoform 83 de CRISON)
  • Binocular microscope (LEICA CM E BA 220/20 W)
  • pH Meter (GLP 21 de CRISON)
  • NPT7 Gasometer analyzer (RADIOMETER)
  • i-STAT® 1 Analyzer (ABBOTT)
  • Veterinary Hematology Analyzer (MINDRAY BC-5300VET)
  • ABL80 FLEX Gasometer Analyzer (RADIOMETER)
  • Urine Analyzer (DIRUI H-100)
  • MAGLUMI, Chemiluminescence Analyzer (Snibe)
  • Ultrafreezer -80ºC (Froilabo)
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U19-E23. Ultrafreezer -80ºC (Froilabo)

Ultrafreezer -80ºC (Froilabo)

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