Small equipment for protein separation
Description: Under this concept, the Unit has the following instruments:
- High-performance liquid chromatography (HPLC) system with UV detector (Agilent 1200 Series), for the chromatographic separation of proteins by reversed phase, ion exange, gel filtration or affinity.
- Two IPGPhor systems (GE Healthcare), for the separation of proteins by isoelectric focusing.
- Protein electrophoresis units: MiniProtean (Bio-Rad), SE600 Ruby (GE Healthcare) y Ettan™ DALTsix (GE Healthcare), for running gels up to 24×26 cm to separate proteins according to their size.
- Microfraction collector and spotter SunCollect (SunChrom), to collect fractions coming out of the nLC and deposit themo onto MALDI plates for mass spectrometry.
Applications: Separation of proteins in liquid or gel supports, according to a wide variety of physicochemical properties.