JUMISC

JUMISC OBTAINS TWO INTERNATIONAL RECOGNITIONS AT THE ANNUAL MEETING OF THE LAPAROSCOPIC SURGEONS SOCIETY HELD IN NEW YORK

Dr. Francisco Miguel Sánchez Margallo, Assistant Director of NANBIOSIS and Scientific Director of JUMISC, has attended the multidisciplinary Congress organized by the Society of Laparoendoscopic Surgeons (SLS), held in New York from August 29 to September 1, where he has presented scientific communications with very innovative results in the specialty of urology.

At the annual meeting of this Scientific Society, international specialists related to minimally invasive surgery meet, with the aim of increasing knowledge of laparoscopic, endoscopic and minimally invasive surgical techniques, including robotic surgery.

The papers presented by Dr. Francisco M. Sánchez Margallo, have received the Harrith M. Hasson Award for the best oral communication, for promoting Teaching and Training in the specialty of urology. He has received this award from the wife of Dr. Hasson, one of the pioneers in laparoscopic surgery and inventor of the open laparoscopic technique and the trocar that bears his name.

The objectives of this work are focused on developing a platform for training and surgical assistance in urology, based on mixed reality technology. In addition, an application with this technology is incorporated for viewing, in real time, by surgeons of tomography, resonance and three-dimensional models of patients.

The results of this work have been tested by different experts of the Spanish Association of Urology (AEU) in the operating rooms of the Center, through an application that allows viewing, in real time, the preoperative studies of tomography, resonance and a human pelvic model. in 3D, through a recreation in the Hololens mixed reality glasses, marketed by the Microsoft company.

The second of the papers presented by Dr. Sánchez Margallo has received a mention of scientific honor, showing the results of the analysis of surgical skills and ergonomic studies of surgeons during the use of a novel robotic device in laparoscopic urological surgery.

These studies have involved different professionals from the clinical and technological areas of the Center and collaborators of the Biomechanics group of the University of Extremadura (UEX) and the CIBEX technology company, in addition to the advice of various Spanish urologists.

The Jesús Usón Minimally Invasive Surgery Center Foundation is a unique infrastructure that works in research, training and innovation in the health field.

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COURSE FOR THE PERFORMANCE OF FUNCTIONS A, B AND C IN RODENTS, LAGOMORFOS, CARNIVORES, PIGS AND SMALL RUMINANTS

On November 2th-30th, Centro de Cirugía de Mínima Invasión Jesús Usón, partner of NANBIOSIS, organizes a theorical and practical course: “Course for the performance of functions a, b and c in rodents, lagomorfos, carnivores, pigs and small ruminants”. Some researchers of NANBIOSIS Units at JUMISC, will transmit their knowhow and experience to the attendees.

The course objetives are to provide adequate training to researchers in the science and welfare of the laboratory animal, as well as the use of bloodless methods of euthanasia that will be carried out with experimental animals in accordance with current legislation and existing recommendations.

This course, once met all the requirements demanded by the Competent Authority (supervised work period) by the students, will provide accreditation for the performance of FUNCTIONS A (care of animals), B (euthanasia of animals) and C (realization of procedures), granted by the Ministry of Environment and Rural, Agrarian Policies and Territory. These functions correspond to those established in article 15, point 2 letters a), b) and c) of Royal Decree 53/2013, of February 1, which establishes the basic rules applicable for the protection of animals used in experimentation and other scientific purposes, including teaching

Program

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Use of Nanomedicine in preclinical studies and animal models

In this video, Beatriz Moreno Lobato, Head of Unit 19 of NANBIOSIS, Clinical Tests Lab and head of the Animal Modeling Service and Clinical Analysis Laboratory of CCMIJU, gives a talk about the use of Nanomedicine in preclinical studies and animal models. Models of type I and II diabetes, cutaneous ulcer models, animal models in ophthalmology, urology models, cartilage defect models or bone defects models and cardiovascular models such as mycardial infarction in pig that Beatriz explains in more detail, as well as implantation of surgical material in combination with adult stem cells

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Testing medical devices and drugs GLP Compliance

En este vídeo Jose Francisco Guillen Caro, Responsable de garantía de calidad del CCMIJU presents the Jesús Usón Minimum Invasion Surgery Center in Cáceres and, especially, the UMISC units that integrate NANBIOSIS, as well as the services and tests they perform, which are certified as GLP

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Science and welfare of the Laboratory animal: New theorical and practical course by JUMISC

From the JUMISC, partner of NANBIOSIS it is been organized: the first edition of the “COURSE FOR THE PERFORMANCE OF FUNCTIONS A, B AND C IN RODENTS, LAGOMORFOS, CARNIVORES, PIGS AND SMALL RUMINANTS”.

This course has the following characteristics:
• Total duration: 98.5 hours, from November 2 to November 30, 2018, with a semi-face-to-face format and mandatory face-to-face practices on November 26, 27 and 28, 2018.
• Cost: € 300
• 25 Places by strict order of registration.

The course is already authorized by the Rural Environment Training Service of the Junta de Extremadura. In addition, accreditation has been requested by the Commission for Continuing Education of the Health Professions of Extremadura. This course, once passed and fulfilled all the requirements demanded by the Competent Authority (qualification required and period of work under supervision) by students, will provide accreditation for the performance of FUNCTIONS A (care of animals), B (euthanasia of the animals) and C (realization of the procedures), granted by the Ministry of Environment and Rural, Agrarian Policies and Territory. These functions correspond to those established in article 15, point 2 letters a), b) and c) of Royal Decree 53/2013, of February 1, which establishes the basic rules applicable for the protection of animals used in experimentation and other scientific purposes, including teaching.

For further information click here

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Seminal plasma influences the fertilizing potential of cryopreserved stallion sperm

Beatriz Macías García, researcher at NANBIOSIS U23. Assisted Reproduction, is co-author of the article “Seminal plasma influences the fertilizing potential of cryopreserved stallion sperm”, publish by the Journal Theriogenology.

Seminal plasma (SP) contains proteins that may influence cryosurvival and prevent capacitation-like changes due to freezing and thawing. The objective of this study was to investigate the effect of adding pooled SP from “good” (GF) or “bad” (BF) freezer stallions on sperm cells’ fertilizing ability. “Good freezers” refers to stallions that usually produce ejaculates which can withstand cryopreservation, whilst “bad freezer” stallions produce ejaculates which cannot tolerate the freezing process. A heterologous zona binding assay with in vitro matured bovine oocytes was used to assess the binding ability of equine sperm cells as a possible alternative to artificial insemination trials. The effect of adding SP i) prior to cryopreservation; ii) after thawing of sperm cells selected by single layer centrifugation (SLC); iii) to capacitation medium, was evaluated. Adding SP from GF stallions prior to cryopreservation reduced the mean number of sperm cells bound to the zona pellucida (ZP) compared to control (P = 0.0003), SP-free sperm cells and group received SP from BF stallions (P ≤ 0.0001 for both). After thawing SLC-selected sperm cells treated with 5% SP showed a decrease in binding ability compared with SP-free sperm cells (P ≤ 0.0001). The binding affinity of sperm cells was higher in the group treated with SP from GF than with SP from BF stallions (P ≤ 0.05). Prolonged exposure to SP impaired the ability of stallion sperm cells to undergo capacitation and bind to ZP, regardless of the source of SP (P ≤ 0.0001). The response of equine sperm cells to SP is influenced by the ability of the sperm cells to withstand cryopreservation and is affected by the timing of exposure and the origin of SP. Customization of the protocol for individual stallions is recommended to optimize the effect.

The research has been carried out with the participation of the NANBIOSIS Assisted Reproduction Lab which ofer the services of

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Mimicking physiological O2 tension in the female reproductive tract improves assisted reproduction outcomes in pig

Francisco M. Sánchez-Margallo, Assistant Director of NANBIOSIS and Scientific Coordinator o NANBIOSIS U23. Assisted Reproduction, is co-author of the article “Mimicking physiological O2 tension in the female reproductive tract improves assisted reproduction outcomes in pig”, publish by the Journal Molecular human reproduction

The research has been carried out with the participation of the NANBIOSIS Assisted Reproduction Lab that has a 120 m2 laboratory, small animal surgery, clinical analysis support, etc. and it is featured with two intracitoplasmatic micromanipulation equipment of the latest generation with IMSI, Laser and Oosight system, embryo biopsy systems, vision systems of the mitotic spindle, with flow cabinets with stereo-microscopes and heated plates, incubators with different gasses systems, equipment and cryopreservation freeze gamete and embryo, among others

STUDY QUESTION:

Is O2 tension in the pig oviduct and uterus affected by the estrous cycle stage and the animal’s age, and can the outcome of in vitro embryo development be improved by mimicking these physiological values?

SUMMARY ANSWER:

O2 tension within the pig reproductive organs is affected by the animal’s age, and values close to those measured in vivo have a positive impact on embryo development and quality when used during IVF and embryo culture (EC).

Article of reference: doi: 10.1093/molehr/gay008

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JUMISC, partner of NANBIOSIS has hosted PICCOLO Project Face-to-Face Meeting.

During two days meeting, the PICCOLO consortium 4^th face to face meeting has taken place. Project partners reviewed the current state of the development, results, deliverables and future actions.

Inspired in the experimental setting up of the JUMISC, one of the most interesting topics in this meeting was the pre-clinical discussion about the validation plan of the PICCOLO prototypes. The PICCOLO consortium analysed both laboratory and pre-clinical trials that will start at the end of this year. Some detailed protocols and preliminary results of the experimental models for these trials were presented as results of a close collaboration between clinical and technological partners.

It has been a great chance to share know-how between clinicians, technological partners and pre-clinical researchers.

Image of the meeting participants at the face to face meeting in the JUMISC, partner of NANBIOSIS.

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NANBIOSIS Unit 14 of Cell Therapy receives the Research Excellence Accreditation

Dr. Javier García Casado, Scientific Director of NANBIOSIS Unit 14, of Cell Therapy, has been awarded by the General Secretariat of Science, Technology and Innovation, belonging to the Ministry of Economy and Infrastructure of the Autonomous Community of Extremadura, the accreditation of research excellence of the Autonomous Community of Extremadura in the field of Life Sciences, which includes the research areas of Cellular and Molecular Biology, Medical Sciences, Pharmacology and Physiology, Food Science and Technology and other related fields.

The accreditation, which is valid for 4 years, is the recognition of Javier García Casado’s research career, highlighted by his contributions to scientific-technological knowledge, due to the impact and international relevance of his scientific results.

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Parasympathetic tone activity evaluation to discriminate ketorolac and ketorolac/tramadol analgesia level in swine

Francisco M. Sánchez-Margallo, Assistant Director of NANBIOSIS and Scientific Director of JUMISC is co-author of the article “Parasympathetic tone activity evaluation to discriminate ketorolac and ketorolac/tramadol analgesia level in swine”, publish by Anesth Analg. 2018 Jun 14. doi: 10.1213/ANE.0000000000003573. [Epub ahead of print]

Abstract

BACKGROUND:

Evaluation of nociceptive–antinociceptive balance during general anesthesia is still challenging and routinely based on clinical criteria. Analgesic drug delivered may be optimized with parasympathetic tone activity (PTA) monitor. This study compares ketorolac and ketorolac/tramadol balance analgesia using a PTA monitor.

METHODS:

Pain intensity response was assessed using a 0–100 numerical state scale (PTA) after nociceptive stimuli in pigs under stable sevoflurane anesthesia. Bispectral index, heart rate, noninvasive blood pressure, and respiratory parameters were also measured. Animals were divided into 3 groups: without analgesia, ketorolac, and ketorolac/tramadol. Mean values or mean areas under the curve (AUC) in selected time periods were compared over time and between groups through a mixed-model repeated measures analysis of variance and nonparametric Kruskal-Wallis tests, followed by Bonferroni or Dunn’s multiple comparisons.

RESULTS:

It was observed a significant decrease in the PTA AUC mean value after application of the stimulus in animals treated without analgesia and only with ketorolac. The PTA AUC mean value in the control group was significantly lower than the corresponding mean in ketorolac group. The ketorolac/tramadol group showed the highest PTA AUC mean values, significantly different from those obtained for the other 2 groups, with no significant differences detected over time. Bispectral index means showed no statistically significant differences either over time periods or between different treatment groups. Heart rate showed only a statistically significant increase in AUC mean between without analgesia and ketorolac/tramadol group, in the time period after the stimulus application. Noninvasive blood pressure means showed no statistically significant differences over time and between treatment groups.

CONCLUSIONS:

This study shows that a low dose combination of ketorolac and tramadol is sufficient to block the pain responses induced with a needle holder in pigs 20 minutes after its administration. The PTA monitor was able to clearly recognize the analgesic level between treatments and may be used to optimize analgesic drug delivered

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STUDY QUESTION:

SUMMARY ANSWER:

Abstract