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U16-S07. Evaluation of the adhesion capacities and formation of biolayers of microorganisms on materials. Evaluation of bactericidal and/or bacteriostatic responses of materials. OUTSTANDING

Description:

Evaluation of the adhesion of microorganisms to surfaces, including the evaluation of their viability. Adhesion can be studied under flow or static and at different contact times. Study of biolayers formed on materials after different growth times. Evaluation of bactericidal and/or bacteriostatic responses using viability kits or plate growth.


Applications:

Testing of materials with anti-infective objectives. Testing of compounds with antibacterial purpose.

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U16-S06. Topographic characterization service using optical profilometry OUTSTANDING

This service makes it possible to determine the surface tension of both solids and liquids under controlled temperature and humidity conditions, even locally by means of deposited drops with a volume of around 10-12 L. The service will be provided by specialists in the characterization of materials in dimensions macroscopic or in colloidal systems. The service can meet the needs of business R&D&i, providing support for their controls according to ISO, ASTM, ASME and EUR standards. In addition, it can also serve research groups with detailed reports according to the needs of the study.

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U16-S05. Characterization service by contact angle goniometry OUTSTANDING

This service makes it possible to determine the surface tension of both solids and liquids under controlled temperature and humidity conditions, even locally by means of deposited drops with a volume of around 10-12 L. The service will be provided by specialists in the characterization of materials in dimensions macroscopic or in colloidal systems. The service can meet the needs of business R&D&i, providing support for their controls according to ISO, ASTM, ASME and EUR standards. In addition, it can also serve research groups with detailed reports according to the needs of the study.

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U13-S09.

Compression-shear test cellscale MicroTester G2

Compression and shear test applied to a wide range of biomaterial and soft tissues. Force is measured using the difference between the tip and base displacement and the stiffness of the beam that connects them.
Mechanical and viscoelastic properties can be measured.

Customer benefits

  • Adaptable system to the material stiffness properties.
  • Well define testing protocols.
  • Tests are recorded in high definition.

Target customer

Public and private research groups focused on biomaterials and tissue mechanical characterization.

References

Nicolás Laita, Ricardo M. Rosales, Ming Wu, Piet Claus, Stefan Janssens, Miguel Ángel Martínez, Manuel Doblaré, Estefanía Peña, On modeling the in vivo ventricular passive mechanical behavior from in vitro experimental properties in porcine hearts, Computers & Structures, 292, 2024, 107241, ISSN 0045-7949, Doi: 10.1016/j.compstruc.2023.107241.

Additional information

Used in BravE project (https://projectbrave.eu/)

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U30-S09.

Data analysis of proteomics experiments

Use of dedicated software for the analysis and interpretation of the results obtained from the proteomic analyses. It includes search engines (MaxQuant, DIANN) for protein identification and quantification, software for statistical analysis (Perseus) and programs for integrated solutions (Peaks) that also enable the analysis of post-translational modifications (PTMs), sequence variants and mutations.

Customer benefits

This service provides comprehensive reports of the data obtained in the proteomic analyses, following the customer’s indications and the objective of each specific project. Upon request, data and reports can be uploaded to private or public repositories.

Target customer

Organizations and research groups focused in biomarker discovery, metaproteomics studies, analysis of specific post-translational modifications, etc.

U30 S09 Data analysis of proteomics experiments
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U30-S08.

Luminex or ELISA assay development and analysis

Design and development of immunoassays employing antibody pairs for the detection and absolute quantification of target proteins. These can be set up on a traditional ELISA format, or coupled to magnetic beads (xMAP, Luminex) for multiplex detection. Optimization procedures, including cross-reactivity assays, are performed to standardize the assay protocol. Alternatively, this service may also use commercial ELISA or multiplex kits for the analysis of specific protein panels.

Customer benefits

This service provides quantitative data of proteins and small protein panels, including cytokines, chemokines or specific biomarker candidates, in biological samples. The assays are run with high sensitivity and throughput.

Target customer

Organizations and research groups that aim to quantify in an absolute way single proteins or small panels in large number of samples. Groups interested in cytokine or chemokine profiling.

U30-S08 : Luminex or ELISA assay development and analysis
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U30-S07.

Protein separation by one- or two-dimensional gel electrophoresis

Separation of complex protein mixtures on isoelectric focusing strips (according to their charge) and/or SDS-PAGE gels (according to their size), as a step for further protein or proteomic analysis.

Customer benefits

This service allows the fractionation of complex protein samples prior to proteomic analysis, performing gel-based quantitative proteomic studies by DIGE (differential in-gel electrophoresis), and evaluating protein profiles by SDS-PAGE followed by gel staining.

Target customer

Organizations and research groups interested in protein or proteomic analysis of biological samples.

U30-S07 Protein separation by one- or two-dimensional gel electrophoresis
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U30-S06.

Protein or peptide separation by liquid chromatography

Separation of complex protein or peptide mixtures using chromatography columns. The chromatographic services offered by the Unit include strong cation exchange (IEX), reversed phase (C18), gel filtration and immunoaffinity for the removal of the top 14 most abundant proteins in human plasma.

Customer benefits

Fractionation of complex protein or peptide samples prior to proteomic or protein analysis. Processing of serum, plasma and other biofluids for shotgun proteomics analysis through the depletion of their most abundant proteins.

Target customer

This strategy is employed to verify or validate biological hypotheses with the highest specificity, as it detects the protein sequence and is not dependent on antibodies.

U30 S06 Protein or peptide separation by liquid chromatography
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U30-S05.

Targeted proteomics analysis by MRM or PRM-MS

Relative or absolute quantification of individual proteins or protein panels by the analysis of their peptides together with stable isotope labelled (SIL) standards.

Customer benefits

This strategy is employed to verify or validate biological hypotheses with the highest specificity, as it detects the protein sequence and is not dependent on antibodies. The use of isotope labelled standards allows the absolute quantification of the protein/s of interest.

Target customer

Organizations and research groups interested in the analysis and quantification of a protein or panel of proteins with high specificity.

U30-S05 Targeted proteomics analysis by MRM or PRM-MS
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U30-S04.

Quantitative shotgun proteomics by nLC-MS/MS

Large-scale relative quantification of proteins in biological samples by nano-flow high performance liquid chromatography combined with mass spectrometry. It can use either data-dependent (DDA) or data-independent (DIA) acquisition modes. Quantifications can be performed via label-free (LFQ) or protein/peptide isotopic labelling using SILAC, iTRAQ or TMT reagents.

Customer benefits

The output of this analyses are lists of proteins identified in the samples, together with quantitative information obtained from the area displayed by each peptide in the mass spectra, and can then be compared across experimental conditions.

Target customer

Organizations and research groups interested in biomarker discovery, drug discovery (protein target identification), development of precision medicine approaches, etc.

U30-S04 Quantitative shotgun proteomics by nLC-MS/MS
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