Quantitative shotgun proteomics by nLC-MS/MS
Large-scale relative quantification of proteins in biological samples by nano-flow high performance liquid chromatography combined with mass spectrometry. It can use either data-dependent (DDA) or data-independent (DIA) acquisition modes. Quantifications can be performed via label-free (LFQ) or protein/peptide isotopic labelling using SILAC, iTRAQ or TMT reagents.
Customer benefits
The output of this analyses are lists of proteins identified in the samples, together with quantitative information obtained from the area displayed by each peptide in the mass spectra, and can then be compared across experimental conditions.
Target customer
Organizations and research groups interested in biomarker discovery, drug discovery (protein target identification), development of precision medicine approaches, etc.