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New equipment adquired to improve NANBIOSIS U20

NANBIOSIS, U20. In Vivo Experimental Platform, led by Dr. Ibane Ibasolo, has been updated and improved as a result of its participation in the project FICTS1420-20, selected by the MICINN for co-financing by the FEDER Program in ICTS 2014-2020 (Equipment for setting standardized immunotoxicology assays for the U20 -NANBIOSIS I17 Action of the Investment Plan-)

A particle particle analyzer equipment (Exoid) has been inslalled at NANBIOSIS U20 for measurement of the concentration and the size of nanoparticles. The Exoid is the latest instrument from the Izon company to measure the physical characteristics of nanoparticles in electrolytic solution. Based on Tunable Resistive Pulse Sensing (TRPS) principles, the equipment is capable of measuring the size of the particles, their concentration and Z potential, through the use of a nanopore that allows measurements to be made in a defined size range.

Finally, a new upright laboratory freezer Liebherr _ SFNe 5227 has been adquired for the storage of the samples and reagents used in the different services of FVPR/U20,

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Representatives of the Ministry of Science and Innovation visited unit 16 of NANBIOSIS

On May 16 representatives of the General Subdirectorate of Large Scientific-Technical Facilities of the Ministry of Science and Innovation visited unit 16 of NANBIOSIS (created by Centro de Investigación Biomédica en Red -CIBER-, and the University of Extermadura).

Mrs. Beatriz Albella Rodríguez and Mrs. María Jesús Marcos Crespo, got to know first-hand the facilities of unit 16 of the CTS NANBIOSIS of “Surface Characterization and Calorimetry .

The visit was guided by the Scientific Director of Unit 16 of NANBIOSIS, Maria Luisa González Martín, and by Mr. Javier de Francisco Morcillo, Director of the Secretariat for Scientific Infrastructure and Technological Development Vice President for Research and Transfer of the University of Extremadura

The reason for the visit was to receive information on the use of European Regional Development Funds that have served to improve the infrastructures of the Spanish Map of Singular Scientific-Technical Infrastructures (ICTS, in Spanish) to support scientific research.

During the last years, this unit 16 of NANBIOSIS ICTS has enlarged its capacities through the project FICTS1420-14-09, an investment of 1.3 million euros, co-financed with FEDER funds, the Ministry of Science and Innovation, and Junta de Extremadura, Regional Ministry of Economy, Science and Digital Agency.

This Unit 16, located in Badajoz, offers the performance of tasks of physical-chemical characterization of surfaces using techniques such as ellipsometry, calorimetry, X-ray photoelectronic spectroscopy (XPS), and detection of secondary ions by means of mass spectrometry by time of flight (Tof-SIMS).

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NANBIOSIS U16 enlarges its capacities with a new FlexPS-ARPES-E kit

NANBIOSIS unit 16 Surface Characterization and Calorimetry Unit form CIBER-BBN and University of Extremadura has recently incorporated new equipment acquired through the execution of the project FICTS1420-14-09, cofinanced with FEDER funds, the Ministry of Economy and Competitiveness and Junta de Extremadura, Regional Ministry of Economy, Science and Digital Agency. A new FlexPS-ARPES-E kit, manufactured by SPECS, is now available for use by researchers

This new resource uses the technique of photoelectron spectroscopy generated by X-rays to analyze the chemical composition of a surface. The electrons given off by the irradiated surface generate a fingerprint of the molecules that make up that surface, yielding information about the chemical composition of approximately the first 10 nm (one millionth of a millimeter) of thickness, making it a technique of Last generation.

One of the advantages of the acquired equipment is that the type of analyzer it has allows for different configurations to obtain different measurement modes. Thus, depending on the application, you can choose between:

XPS/ESCA (X-ray Photoelectron Spectroscopy) mode: The excitation source is X-ray.

SEM/SAM (Scanning Electron Microscopy/Auger) mode: the excitation source is electrons.

UPS mode (Ultraviolet Ray Photoelectron Spectroscopy): The excitation source is UV rays.

ISS (Ion Scattering Spectroscopy) mode: the excitation source is ions. This excitation source can also be used for depth profiling.

At a cost of 800,000 euros, its acquisition has been achieved thanks to financial support from the Ministry of Science and Innovation, the General Secretariat for Research and FEDER funds from the multi-regional operational program of Spain in the line of action of Singular Scientific and Technical Infrastructures ( ICTS). In addition, it has been co-financed by the Junta de Extremadura, the Ministry of Economy, Science and Digital Agenda and the General Secretariat of Science, Technology, Innovation and University.

Other equipment financed in this same action is:

A DMC8 Leica profilometer: a device that allows determining the 3D texture of surfaces through spatial, volumetric and height parameters, from the millimeter to the nanometer range.

A Krüss DSA100E/ DSA100M goniometry : system for determining the surface tension of solids and liquids. The equipment has a microdrop dosing system, a thermostatic chamber and a chamber for controlling the vapor saturation of the liquids being analysed. In addition, the microdroplet system has a tilting base that allows the samples to be tilted by at least 90°.

An additional cannon for a team of TOF-SIMs: a team of secondary ion mass spectrometry by time of flight (TOF-SIMS), a very sensitive technique for analyzing the composition of surfaces that provides detailed elemental and molecular information of coatings, layers fine lines and interfaces both at the superficial and three-dimensional level.

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New equipment installed in NANBIOSIS U20

NANBIOSIS, U20. In Vivo Experimental Platform, led by Dr. Ibane Ibasolo, has been updated and improved as a result of its participation in the project FICTS1420-20, selected by the MICINN for co-financing by the FEDER Program in ICTS 2014-2020 (Equipment for setting standardized immunotoxicology assays for the U20 -NANBIOSIS I17 Action of the Investment Plan-)

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Filtered Air Shower for Animal Housing

Cáceres, September 10th, 2021

In the framework of the project “Updating the Infrastructures and implementation of a LIMS document control and management system to enhance the capacities of the Distributed ICTS NANBIOSIS Units managed by the CCMIJU (AILIMS-NANBIOSIS)  a filtered air shower for Animal Housing has been bought with strictly controlled environmental parameters, to prevent possible cross-contamination and the entry of pathogens and viral particles, since during  entry and exit of personnel to this area, contamination by the mentioned particles has been detected, so the air shower is an effective solution to eliminate them and protect the controlled environment.

AILIMS has been co-funded by the European Regional Development Fund (FEDER) within the framework of the Pluriregional Operational Program of Spain (POPE) of Singular Scientific and Technical Infrastructures (ICTS) 2014-2020 and the acquisition of the shower is part of the first action of the following two, financed with FEDER Funds:

-Within Unit 22 or Animal Facility, the Installation and development of an environmental treatment-control system of the housing rooms, an Improvement and adaptation of the spaces for experimental models, the Acquisition, installation and development of a two-door ultra- filtered air shower and the Acquisition, installation and development of a post-procedural recovery chamber.

– For the rest of the NANBIOSIS Units managed by the CCMIJU, Acquisition, installation and development of a LIMS Software.

The actions and their budget are here detailed: (VI) PROJECT REFERENCE: ICTS-2019-14-46), together with the FEDER co-funded rate.

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New leaflets available of new equipment at NANBIOSIS Unit 16

We are delighted to announce the publication of our new brochures which reflects the new equiments and capabilities incorporated to NANBIOSI U16 Surface Characterization and Calorimetry Unit.

The equipment is available has been incorporated to the Unit thanks to European Regional Development Fund (FEDER) allocated by the Goberment of Spain for the development of ICTSs, specifically through the Project FICTS-1420-14-09.

Further information: NANBIOSIS News

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Agreement signed with Spanish Government for the allocation of FEDER funds for Nanbiosis ICTS

In the framework of the FEDER Program in ICTS 2014-2020, a projects related to the ICTS NANBIOSIS has been selected by the Ministry of Science, Innovation and Universities for co-financing with FEDER funds of the European Regional Development Funds program.

An agreement has been signed between Ministry of Science, Innovation and CCMIJU, institution that houses NANBIOSIS  U14, U19, U21, U22, U23, U24 for the co-financing of the Project ICTS-2019-14-46: “ACTUALIZACIÓN DE LAS INFRAESTRUCTURAS E IMPLEMENTACIÓN DE UN SISTEMA DE CONTROL DOCUMENTAL Y DE GESTIÓN LIMS PARA POTENCIAR LAS CAPACIDADES DE LAS UNIDADES DE LA ICTS DISTRIBUIDA NANBIOSIS (AILIMS-NANBIOSIS)”

 The total budget of the project amounts to € 597.000, with 80% financing with FEDER Funds.

CCMIU is processing the necessary contracting procedures for the execution of this project.

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New equipment for calorimetry and surface characterization for NANBIOSIS U16

NANBIOSIS unit 16 Surface Characterization and Calorimetry Unit form CIBER-BBN and University of Extremadura offers the performance of tasks of physical-chemical characterization of surfaces using techniques such as ellipsometry, calorimetry, X-ray photoelectronic spectroscopy (XPS) and detection of secondary ions by means of mass spectrometry by time of flight (Tof-SIMS). Recently, new equipment acquired through the execution an investment of 1.3 million euros, cofinanced with FEDER funds, the Ministry of Economy and Competitiveness and Junta de Extremadura, Regional Ministry of Economy, Science and Digital Agency, througth the project FICTS1420-14-09. These equipments are a microdroplet and picodroplet contact angle goniometry system and an optical profilometry system.

Equipment acquired

PEAK AND MICRO DROP GONIOMETRY SYSTEM

This system allows to measure contact angles automatically, controlling by software, the deposition of drops of different liquids, their analysis and the orientation of the substrate, as well as pending drops. In addition, the microdroplet system has a tilting base that allows the samples to be tilted by at least 90o. It also includes a thermostatic chamber, for temperature changes of the sample with a range between 5 and 90 ºC, and a chamber for humidity control.

Obtaining surface tension, through contact angle measurements, is a factor to consider in technologies of biomedical interest such as implants and other materials that must be in contact with biological fluids. In these systems the contact angle is related to the wettability, the hydrophobicity of the surface and the adhesion capacity of substances such as proteins or other compounds on the surfaces.

In the case of pico-droplet measurements, the system allows to very precisely dose drops much smaller than in the previous case, which can be as low as 20 picoliters, as well as to analyze their shape to measure the contact angle. This fact solves the problem of measuring wettability in very small structures, such as capillaries, microchips, joints created in the union of two materials, etc.

PROFILOMETRY SYSTEM

The system allows the roughness of a multitude of surfaces to be measured by an optical method that does not make any changes to the sample. With the operating base of a confocal microscope commonly used in different fields of science, it allows to create high-resolution 3D images quickly and automatically, as well as obtaining color images thanks to the use of three LEDs: Red, Green and Blue. With the available objectives, it is possible to measure from more macroscopic samples such as screws used as dental implants to be able to observe bacterial colonies composed of bacteria the size of a micron. This will make it possible to measure the roughness of any sample covering the range of roughness between a magnifying glass, which gives a more macroscopic view, and the atomic force microscope capable of measuring nanometric roughness (10 ^ -9 m). In addition, this equipment also works as an interferometer that allows to measure the roughness with greater precision of mirror polished samples in a simple way, obtaining images of higher resolution than any confocal technique.

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