U14-S02. Flow Cytometry (Onsite&Remote) OUTSTANDING
Cellular phenotyping, cytokine assays, profiferation assays, viability and apoptosis.
+34 620 10 75 37info@nanbiosis.com
Cellular phenotyping, cytokine assays, profiferation assays, viability and apoptosis.
Isolation and expansión of mesenchymal stem cells; viability assays and cytotoxicity, porcine mesenchymal stem cells (isolated from adipose tissue, bone marrow and synovial fluid) for preclinical trials. Murine mesenchymal stem (isolated from adipose and bone marrow).
Cell Culture Room 1:
Vertical Laminar Flow Cabinet Herasafe® (Formimetal).
CO2 incubator Heracell 150 (Thermo)
Nomarski phase inverted microscope and fluorescencia con cámara (Nikon)
Camera Color 1CCD (Nikon)
Cooled Camera black and white (Nikon)
Vacuum Pump ME4 (Vacuubrand)
Cell culture water Baths (Controltecnica)
Cell Culture Room 2:
Vertical Laminar Flow Cabinet Herasafe® (Formimetal)
CO2 incubator Heracell 150 (Thermo)
CO2 incubator Heracell 150 with CO2 control (Thermo)
phases inverted microscope with fluorescence (NIKON)
Vacuum Pump ME4 (Vacuubrand)
Cell Counter (Invitrogen)
Molecular Biology
Fume Hood (Formimetal)
Stereo microscope with camera and digital image set (NIKON)
light source (for the glass) (NIKON)
Refrigerated centrifuge for microtubs Biofuge® Pico (HERAEUS)
Digital circulation thermostatic bath (GRANT)
Thermostatic bath (for cell cultures temperar medios) (MEDIGEN))
Pipette controller pipette Powerpette-plus (IZASA)
Pipettes de volumen variable continuo with accessories (RAININ)
Vertical Freezer -20ºC (FIOCHETTI)
Freezer +2/+8ºC (CLASS ELECTRONIC)
Laboratory vertical freezer Herfreeze ®HFU-486-Basic (-80ºC) (HERAEUS)
Crioconservtion system with accessories
Liquid Nitrogen tank (taylor wharton)
Thermocycler (THERMO)
Sterilization incubator (Modelo UT ) (HERAEUS)
G-Box Translumitator and accessories (PC, Sofware…) (SYNGENE)
Electrophoresis system (IZASA)
Electrophoresis vertical jar
Electrophoresis horizontal (big) jar
Electrophoresis horizontal (small) jar
Rotary shaker with incubation (HEIDOLPH)
Magnetic stirrer with heating (ARE)
Analytical balance 210gr, 0,01 gr. (ACCULAB)
Precision analytical balance (OHAUS)
Ultrasonic water bath (SELECTA)
Crushed ice machine (Schottman)
Microwave (LG)
Pipette controller (POWERPETTE PLUS)
Thermoblock (Labolan)
Vortex 230 V, EU (VWR)
Freezer +4ºC/-20ºC
UV mini-1240 Spectrophotometer (SHIMADZU)
ELISA plate reader, Synergy MX (BIOTEK)
Flow cytometry FACSCalibur (BD)
pH-Meter (Symphony)
i-Blot (Invitrogen)
Autoclave
Machines for mechanical tests:
• Instron MicroTester 5848 and 5548: electromechanical machines for displacement or load control tests:
•• load range from 1mN to 2kN at full scale.
•• load cells of:+/- 5, 50, 500 and 1000N.
•• synchronous data capture in all the channels up to
500 Hz.
• Biaxial Instron MicroTester 8874: axial-torsional, servohydraulic testing machine:
•• load capacity of 25 kN and 100 Nm.
•• fatigue tests at a low number of cycles.
›› Software for data control and analysis.
›› Accessories and tools especially designed by the group, which allow a wide flexibility in designing tests, allowing the verification and/or validation of theoretical models.
›› AVE video extensometer connected to the machines; resolution of 0.5 μm. To determine the axial and transverse deformation without contact (tension, dynamic properties and viscoelasticity) of the samples.
• E1000 Test Instrument: Dynamic systems to offer slow-speed static testing and high-frequency dynamic fatigue testing with hundreds of Hertz capability: Load range: 250 N to 2.0 KN.
• Nano BIONIX MTS: For nanomechanical characterization of biomaterials. Load range to 0.001N to 0.5N.
Temporarily OUT OF ORDERMicroCT GE eXplore Locus SP (GE): Computed X-ray Axial Tomograph for the non-destructive study of the microstructure and in vitro tomography: ›› Samples of up to 40 mm in diameter; resolution of 10 μm. ›› Different types of tissues, implants, scaffolds, etc. ›› Complete “section-by-section” imaging with a single rotation (cone beam volumetric technology); excellent image quality and “signal-to-noise” ratio. ›› “Bone Analysis Tool” software provided with: •• BMD (Bone Mineral Density): It calculates the density of minerals in the bone, the bone volume fraction and the mineral content in the bone. •• Anisotropy: It determines the degree of symmetry and the orientation of the trabecular structure. •• Cortical Analysis: It determines “section-by-section” the width, surface area and the BMD value of the cortical bone. •• Stereology: It provides the bone volume fraction, the ratio between the surface of the bone and its volume and the width of the trabecular plate. •• Direct Measures: It determines the local trabecular width of a bone and allows obtaining a visual representation of this local width.
X-Ray image for biomaterials, scaffolds and biological tissues.
Customer benefits
An axis inside is able to apply forces.
Non-destructive view of internal structure pre and post force.
Target customer
Public and private research groups focused on biomaterials and tissue mechanical characterization.
Additional information
Fatigue tests allow us to know how is the behaviour when cyclic load are applied. It is used for life expectancy of biomaterials and biological tissues.
Customer benefits
High full-scale cell load.
Target customer
Public and private research groups focused on biomaterials and tissue mechanical characterization.
Additional information
Dynamic tests allow us to know how is the behaviour along time. It is used for viscoelastic properties of biomaterials and biological tissues.
Customer benefits
High full-scale cell load.
Target customer
Public and private research groups focused on biomaterials and tissue mechanical characterization.
Additional information
Tensile, pure shear and planar tension are the tests inside the uniaxial group. Samples are stretched along an axis by measuring force and displacement. Cycling protocols can be integrated into the mechanical test. Mechanical and viscoelastic properties can be studied. Force is measured by means of load cells.
Customer benefits
Target customer
Public and private research groups focused on biomaterials and tissue mechanical characterization.
References
Additional information
