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U2-S08. Inmunochemical methods development (Remote) OUTSTANDING

CAbS offers personalized development of immunoassays. Antibodies and other necessary immunoreagents can be synthesized.

The development of the immunoassay includes different stages:

  1. Design of the immunoassay format, sample matrix, the sensibility and the working range. Identification of necessary reagents and their conditions to obtain optimal results.
  2. Development Phase. Establishing assay parameters, for example the detection limit and working range. Evaluation of the effects of the matrix on the assay. Reproducibility study.
  3. Assay validation. Evaluation of the stability and robustness of the reagents. Measurement of inter and intra-assay reproducibility, precision and specificity. Validation with blind samples. Assay platforms: ELISA, microarrays with different formats based on the detection of individual analytes or multiplexed assays.
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U2-S07. Antibody purification (Remote) OUTSTANDING

The purification of the antibodies includes the isolation of the antibody from the serum (polyclonal antibodies) or culture supernatant from hybridoma cell lines (monoclonal antibodies). The services offers previous advise on the different possibilities for purification according to the needs of the user.

The methods of purification that we offer vary from crude methods (precipitation of the proteins from the sample including any antibody present) to general purification (purification by affinity of certain classes of antibodies without taking account of the specificity to the antigen) to specific (purification by affinity of the antibodies which join specifically to a definite antigen).

  • Ammonium sulfate precipitation
  • Protein A, protein G affinity purification
  • Specific antigen affinity purification
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U2-S06. Hybridoma cell culture antibody production (Remote) OUTSTANDING

Hybridoma cell culture in ultralow serum medium. We use CELLine™  Bioreactor Technologys to produce monoclonal antibodies.

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U2-S05. Monoclonal Antibody development (Remote) OUTSTANDING

The mAb development contains the following phases:

  1. Immunisation of mice, titre determination in the antiserum.
  2. Cultivation of the myeloma cell line, cell fusion, selection in HAT medium and screening.
  3. Cloning , screening and cultivation of positive cultures. Further cloning and obtaining stable clones.
  4. Expansion and Cryo conservation of the selected clones. Delivery of cell culture supernatants and frozen hybridoma cells.
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U2-S04. Polyclonal Antibody Production (Remote) OUTSTANDING

The service has a standard immunization protocol that includes monthly inoculations with Freund adjuvant during a period of 4 months. This protocol can be adapted to final project objective.

To determine the progress of the immune response, a bleed is taken after 10 days after the second immunization to obtain antiserum and the titer is determined through ELISA.

Among the controls is included obtaining preimmune serum from the animals obtained from the blood previously to the first injection. At the end of the process 50-70 mL of hyperimmune serum is obtained per animal from final production bleed.

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U2-S03. Preparation of Bioconjugates and Molecular Probes (Remote) OUTSTANDING

Protein and hapten conjugation services with different types of molecules such as: Enzymes, Fluorophores, Biotin, KLH, BSA, gold particles, magnetic particles, nanotubes…

Preparation of antibody fragments.

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U2-S02. Hapten synthesis (Remote) OUTSTANDING

Selection of suitable leading structures for antigen preparation, hapten synthesis, hapten modification and derivation.

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Strong Ministerial Support to NANBIOSIS

MINECO grants a “Network of Excellence” with the maximum funding of 178,000 euro to consolidate the unified management model of NANBIOSIS, to boost its internationalization and strategic positioning as well as to promote a complete cascade service for nanomaterials characterization.

The two years lasting project has been granted within the frame of the National Programme for Fostering Excellence in Scientific and Technical Research.

This Excellence project, funded by the Spanish Ministry of Economy and Competitiveness, will consolidate and promote the One Stop Shop model of NANBIOSIS, which facilitate to public and private centers of research and companies a simply and competitive access to an offer that ranges from the product´s design and procurement itself, its development and characterization up to the complete pre-clinical validation.

The actions of this project are also aimed at achieving other objectives include: Improve governance and coordination within the network, transfer of knowledge to the company and innovation, boost the competitiveness of Spanish infrastructures in the international context…. This, has been scheduled a series of actions including, establish standardized administrative processes and protocols, regular meetings between members of the Coordination Committee and the advisory committee; developing a Marketing Plan, organization of forums with companies and hospitals, assisting partnering events, training, mobility grants for researchers and generating a new internal structure with selected units of NANBIOSIS to offer through a single contact point, a cascade service package for characterization and advisory (“cascade assay services”) to external users (manufacturers, hospitals, academic and institutional).

Nanbiosis-concesión Redes
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Concepció Rovira from ICMAB (CSIC/CIBER-BBN) has been awarded with the Prize of Research Excellence of the RSEQ

Concepció Rovira from ICMAB (CSIC/CIBER-BBN) has been awarded with the Prize of Research Excellence of the RSEQ (Spanish Royal Society of Chemistry). The prize was delivered by Marina Villegas, General Director of Research of MINECO and Jesús Jiménez-Barbero, President of the RSEQ.

The Prizes of the RSEQ in 2015, in the modality of Medal of the RSEQ, Prizes of Research Excellence and Prizes of Dissemination have been for:

Medal of RSEQ:

  • Prof. José Luis Mascareñas Cid (Centro Singular de Investigación en Química Biológica y Materiales Moleculares (CIQUS) – Universidad de Santiago de Compostela)

Prizes of Research Excellence:

  • Prof. Nuria López Alonso, Instituto Catalán de Investigación Química (ICIQ).
  • Prof. Rubén Martín Romo, Instituto Catalán de Investigación Química (ICIQ).
  • Prof. Kilian Muñiz Klein, Instituto Catalán de Investigación Química (ICIQ).
  • Prof. Concepció Rovira Angulo, Instituto de Ciencia de Materiales de Barcelona (ICMAB-CSIC).
  • Prof. Félix J. Zamora Abanades, Universidad Autónoma de Madrid, (UAM).

Prizes of Dissemination:

  • Dña. María Luisa Prolongo Sarria, I.E.S. Manuel Romero. Villanueva de la Concepción, Málaga

 

 

Nanbiosis-U6-Concepció Rovira from ICMAB Prize of Research Excellence of the RSEQ
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U2-E08. Laboratory perfectly equipped for chemical synthesis (to hapten synthesis)

Laboratory perfectly equipped for chemical synthesis (to hapten synthesis)

Laboratory immunoassays E08
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