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Posts Taged myocardial-infarction

Intracoronary Delivery of Porcine Cardiac Progenitor Cells Overexpressing IGF-1 and HGF in a Pig Model of Sub-Acute Myocardial Infarction

Researchers of NANBIOSIS units U14 Cell Therapy unit, U21 Experimental operating rooms and U24Medical Imaging at JUMISC are coauthors of an article recently published by the journal Cells “Intracoronary Delivery of Porcine Cardiac Progenitor Cells Overexpressing IGF-1 and HGF in a Pig Model of Sub-Acute Myocardial Infarction“·

Large animal studies have been carried out by the ICTS ‘NANBIOSIS’, specifically Units 14, 21, 22, and 24 of the JUMISC

Human cardiac progenitor cells (hCPC) are considered a good candidate in cell therapy for ischemic heart disease, demonstrating capacity to improve functional recovery after myocardial infarction (MI), both in small and large preclinical animal models. However, improvements are required in terms of cell engraftment and efficacy. Based on previously published reports, insulin-growth factor 1 (IGF-1) and hepatocyte growth factor (HGF) have demonstrated substantial cardioprotective, repair and regeneration activities, so they are good candidates to be evaluated in large animal model of MI. We have validated porcine cardiac progenitor cells (pCPC) and lentiviral vectors to overexpress IGF-1 (co-expressing eGFP) and HGF (co-expressing mCherry). pCPC were transduced and IGF1-eGFPpos and HGF-mCherrypos populations were purified by cell sorting and further expanded. Overexpression of IGF-1 has a limited impact on pCPC expression profile, whereas results indicated that pCPC-HGF-mCherry cultures could be counter selecting high expresser cells. In addition, pCPC-IGF1-eGFP showed a higher cardiogenic response, evaluated in co-cultures with decellularized extracellular matrix, compared with native pCPC or pCPC-HGF-mCherry. In vivo intracoronary co-administration of pCPC-IGF1-eGFP and pCPC-HFG-mCherry (1:1; 40 × 106/animal), one week after the induction of an MI model in swine, revealed no significant improvement in cardiac function.

Article: Intracoronary Delivery of Porcine Cardiac Progenitor Cells Overexpressing IGF-1 and HGF in a Pig Model of Sub-Acute Myocardial Infarction Cells 202110(10), 2571

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Outstanding Young Researcher Award at ICESS 2021 to Konstantinos Mountris (NANBIOSIS U27).

Konstantinos Mountris researcher from the BSICoS group of CIBER-BBN and I3A at the University of Zaragoza has been granted the Outstanding Young Researcher Award at the International Conference on Computational & Experimental Engineering and Sciences (ICCES) in relation with the work Radial Point Interpolation Mixed Collocation (RPIMC) Method for The Solution of Reaction-Diffusion Equation in Cardiac Eletrophysiology (for the simulation of myocardial infarction).

This work was already recognized in the Congress of Computing in Cardiology (CinC) held recently where Konstantinos Mountris and Esther Pueyo have received the Maastricht Simulation Award (MSA)Konstantinos Mountris acknowledged the contribution of NANBIOSIS U27 High Performance Computing :“using the HPC services of NANBIOSIS U27 we were able to validate the RPIMC method as a promising alternative to Finite Element Method performing large-scale simulations of myocardial infarction in biventricular swine models

Related news: Understanding human heart behaviour with mathematics and engineering.

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Microencapsulated Insulin-Like Growth Factor-1 therapy improves cardiac function and reduces fibrosis in a porcine acute myocardial infarction model

Researchers of NANBIOSIS Units at JUMISC. led by Verónica Crisóstomo, Scientific Director of NANBIOSIS unit 24 of Medical Imaging, have just published an article in the Journal Scientific Report

Cardiovascular diseases, especially ischemic heart disease, are the leading cause of mortality worldwide. Conventional treatments have contributed to reduce early mortality after an acute myocardial infarction, but do not recover the damaged myocardial tissue. In the last two decades stem cell therapy has been studied for that purpose. Nowadays it is known now that stem cells are able to secrete combinations of biomolecules that modulate the composition of the damaged cardiac environment contributing to functional tissue repair by stimulating the migration, proliferation and survival of endogenous cardiac progenitor cells as well as attenuating fibrosis and modulating inflammation. Among the secreted substances, there are different cytokines, extracellular vesicles and growth factors including insulin-like growth factor-1 (IGF-1). Our researchers’ goal in this work has been to assess the safety and effectiveness of an intracoronary infusion of microencapsulated IGF-1 after acute myocardial infarction in a clinically relevant swine model of reperfused myocardial infarction.

Large animal studies have been conducted by the ICTS “NANBIOSIS”, more specifically by Units 14, 21, 22 and 24 of the Jesús Usón Minimally Invasive Surgery Centre.

Article:

Báez-Díaz, C., Blanco-Blázquez, V., Sánchez-Margallo, F. et al. Microencapsulated Insulin-Like Growth Factor-1 therapy improves cardiac function and reduces fibrosis in a porcine acute myocardial infarction model. Sci Rep 10, 7166 (2020).

https://doi.org/10.1038/s41598-020-64097-y

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Award to the best oral presentation in the 2016 Spanish Society of Cardiology Meeting

The oral presentation entitled “NonInvasive Assessment of an engineered bioactive graft using impedance spectroscopy in a swine myocardial infarction”, presented in the 2016 Spanish Society of Cardiology Meeting, has been awarded.

The Heart Diseases Meeting, held in the town of Zaragoza last 27-29th October, was focused on sharing experiences, making contacts and updating some knowledge.

The event got together cardiologists, general practitioners, researchers, internists, epidemiologists, practitioner residents and nurses, offering more than 140 activities and nearly 1000 communications.

Carolina Gálvez-Montón, Ramón Bragós, Carolina Soler-Botija, Idoia Díaz-Güemes, Cristina Prat-Vidal, Verónica Crisóstomo, Francisco Miguel Sánchez-Margallo and Antoni Bayés-Genís (three of them JUMISC’semployees), were the authors awarded by the best oral presentation.

This is the result of the collaboration between the Germans Trias i Pujol Hospital, the Politécnica of Cataluña University, the Autónoma University of Barcelona and the Jesús Usón Minimally Invasive Surgery Centre (JUMISC).

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Award to the best oral presentation in the 2016 Spanish Society of Cardiology Meeting
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