Cationic nioplexes in supramolecular hydrogels as hybrid materials to deliver nucleic acids
Jose Luis Pedraz, Scientific Director of Unit 10 of NANBIOSIS Drug Formulation unit and Ramon Eritja Scientific Director of Unit U29 of NANBIOSIS Oligonucleotide Synthesis Platform (OSP) (CIBER-BBN) have participated in the entrapment of cationic nioplexes in supramolecular hydrogels and the use of these materials for transfecting cells.
This work is focused on entrapping cationic nioplexes within supramolecular hydrogels based on N-protected phenylalanine. To modulate the supramolecular hydrogel diffusion properties, hydrogels were easily tuned with ĸ-carrageenan (≤ 1%). These materials were fully characterized using rheology. The niosomal liberation in solution through hydrogels was monitored by fluorescence and this release was controlled by diffusion mechanisms. The lack of toxicity of these materials allowed these materials to be used in cell culture. Preliminary transfection results confirmed the suitability of entrapping niosomal formulations in supramolecular hydrogels and the potential opening up of alternative strategies in therapy.
This study was published in RSC Advances:
S. Grijalvo, G. Puras, J. Zárate, R. Pons, J.L. Pedraz, R. Eritja, D. Díaz. “Nioplexes encapsulated in supramolecular hybrid biohydrogels as versatile delivery platforms for nucleic acids” 2016, 6, 39688-39699. DOI: 10.1039/C6RA01005A