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Posts on Jan 1970

U28-E06. Bruker Minispec MQ-60

U28-E06. Bruker Minispec MQ-60

The Minispec TD-NMR is broadly used in research, development and quality control of MRI Contrast Agents. These agents are used to enhance contrast in Magnetic Resonance images between tissues that otherwise would be difficult to differentiate. Most of these contrast agents are based on Gd chelates or iron oxide nanoparticles, which produce signal contrast enhancement by affecting the magnetic relaxation properties of water molecules. The Minispec MQ-60 provides T1 and T2 measurements with high accuracy and reproducibility, and, most importantly, at clinical field, which is fundamental for translational research. Hence, the Minispec MQ-60 plays a key role in the characterization of new contrast agents for clinical use.

Specifications:
Permanent 1.4T magnetic field.
“Intelligent” 1H probe (“plug and play”) with 60 MHz operating frequency
Fast magnet warm-up and precise digital temperature regulation for ultimate results stability
Optical detector to any kind of glass, quartz and plastic samples. Specific filters for natural and fluorescent light suppression and energy saving lamps

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U28-E05. Bruker Avance 600 MHz NMR spectrometer + HR-MAS Probe

U28-E05. Bruker Avance 600 MHz NMR spectrometer + HR-MAS Probe

Specifications:
UltraShieldTM Plus 14.1 T magnet
5 mm BBFOPLUS probe, optimized for X-nuclei direct observation in a frequency range between 31P and 15N. Automated tuning and matching (ATM).
5 mm TXI probe, optimized for higher sensitivity 1H-nuclei observation. The outer coil is optimized for decoupling of 13C and 31P.Automated tuning and matching device (ATM).
TCI CryoProbe Prodigy, uses nitrogen-cooled RF coils and preamplifiers for 1H, 2H and 13C, delivering a 2.5x 1H sensitivity boost. The probe is ATM compatible and has 2H “lock” channel and Z-gradient.
Control temperature unit (from 0 ºC to 50 ºC)

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U28-E04. Bruker Ascend 400 MHz NMR spectrometer

U28-E04. Bruker Ascend 400 MHz NMR spectrometer

Specifications:
UltraShieldTM Plus 9.4T magnet
5 mm BBFOPLUS probe, optimized for X-nuclei direct observation. Broad band in a frequency range between 31P and 15N. This probe has 2H “lock” channel and z gradient that allows 2D spectroscopy and hetero correlation 19F/1H and 1H/19F, to be performed. It also includes an automated tuning and matching device (ATM)
Sample Xpress Lite (for 16 samples)
Control temperature unit (from 0 ºC to 50 ºC)

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U28-E03. Bruker Albira small animal CT system

U28-E03. Bruker Albira small animal CT system

Specifications:
Dedicated beds for individual rats and mice with integrated gas anesteasia.
Albira Software Suite 5.0 with integrated acquisition and tomography software.
PMOD biomedical image quantification software.
X-ray Focal Spot Size (Nominal) 35 μm.
Energy Range (kVp) 10-50.
Field of View (FOV) (transaxial x axial) 70 mm x 70mm
Multiple FOV windows can be combined for whole animal imaging
Bore Size 105 mm

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U28-E02. Bruker In vivo Xtreme

The In-Vivo Xtreme is a multi-modal optical and X-ray small animal imaging system, designed to combine sensitivity, speed and flexibility for the broadest range of applications for molecular imaging. Equipped with a high performance f/1.3 58mm lens and 4MP back-illuminated CCD camera, the In Vivo Xtreme is suitable for demanding bio-luminescence and fluorescence intravital imaging challenges

Specifications:
Back-illuminated 4 MP camera
f/1.1 – f/16, 58 mm fixed lens (on movable platform)
400W Xenon Light Source
28 Excitation Filters 410 nm – 760 nm
Fluorescent imaging from the visible to near IR
Imaging chamber for up to five mice at a time with
integrated anesthesia and temperature control
Automated switching between modalities – the animal never moves
Multimodal Animal Rotation System (MARS)
Integrated high resolution X-ray imaging (>18lp/mm spot size <60μm)
X-ray illumination optimized for small animal imaging with 5 selectable filter settings for fine tuning

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U28-E01. Bruker BioSpec 9.4T animal MRI system

U28-E01. Bruker BioSpec 9.4T animal MRI system


The Biospec 94/20 is a multipurpose research system for high-resolution magnetic resonance spectroscopy and imaging. The system is designed for preclinical and molecular MR imaging. State-of-the-art RF probes technology together with ultra high field USR magnets deliver high spatial resolution in vivo enabling researchers to come closer to the molecular and cellular level (molecular imaging). Thanks to its innovative modular concept, virtually any small animal MR imaging application in life science, biomedical and preclinical research can be conducted.
Specifications:
Magnetic field strength of 9.4T
20 cm bore diameter, suitable for mice and rats
Automated positioning system (AutoPac™)
High performance actively shielded
gradient system with integrated shims
RF coils for rats and mice

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U28-S021. Flow Citometry/Cell Sorter (Onsite&Remote)

Flow cytometry is a highly effective method for analyzing thousands cells or other particles in a matter of minutes. Flow cytometers detect the fluorescence, size and complexity of particles that pass through laser beams as a stream. In this way they can quickly generating statistically valid data by count very large numbers of particles – something that would be extremely difficult with traditional microscopy approaches. Flow cytometers are very well established in hospitals for routine diagnosis and now widely used for different types of research application from marine biology to stem cell research. A Cell Sorter is a specialized flow cytometer which is able to physically separate different classes of particles based on their fluorescence or other physical characteristics.

Applications:
>> Detection of rare events: the high throughput of Gallios allows for the statically-valid detection of very rare cellular events.
>> Multi-parameter assays: Three lasers and ten channels of Gallios allows the design of complex assays using multiple markers.
>> Cell cycle analysis: detection of DNA content, DNA damage, apoptosis and cell cycle antigens and many others.
>> Marine biology: flow cytometry is increasingly used as a tool to study marine microorganisms using their innate fluorescence properties combined with size and complexity characteristics.
>> Single cell separation: Mo-Flo is able to automatically sort single cells into individual wells of a 96-well plate with a very high degree of accuracy and is suitable for colony formation, stem cell isolation etc.
>> Cell purification from tissues: Mo-Flo allows individual cell types from complex tissues to be separated based on size, complexity or markers such as GFP. The yield and purity can be adjusted to suit different applications. Measurement of Relaxation Times (T1, T2, T1*)

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U28-S020. High Content Screening (Onsite&Remote) OUTSTANDING

High content screening (HCS) attempts to bridge the gap between microscopy and cytometry, providing both large quantities of statically useful and phenotypically rich data. High content screening combines automated microscopy with customizable algorithms able to detect and quantify many different types of phenotypic information in both fixed and living cultured cells. Part of the Bionand Cell Culture Unit, the Perkin Elmer Operetta high content screening combines the quality of a spinning disc confocal microscope with Harmony HCS software, which contains many common algorithms such as nuclei and cells detection, as well as the ability to “learn” new phenotypes. Bionand will also offer access to the advanced server-based Columbus HCS software.

Applications:
>> Drug dose response curve calculation: On a single plate multiple concentrations or combinations of compounds can be easily and quickly tested. The software includes all the analysis and presentation tool required to calculate IC50 values. And display response curves
>> Small molecule screens based on changes in cell phenotype: Not all phenotypes can be detected easily by cytometry. Operetta allows very complex phenotypes (e.g. organelle distribution or cell organization changes) to be detected automatically and reliably.
>> Large scale siRNA screens: Automatic detections of up to 384 different siRNAs on a single plate and analysis them automatically makes it possible to perform genome-scale screens for phenotypes of interest
>> Long term cell culture studies: Operetta is equipped with temperature and CO2 control making it suitable for maintaining live cultured cells in optimal conditions during image acquisition

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U28-S019. Fluorescence microscopy (Onsite&Remote)

For many applications conventional widefield fluorescence microscopy is still the best choice. The CCD detected used for conventional microscopy are often more sensitive that the photomultiplier tubes used in confocal microscopes and flow cytometers. As the camera capture the whole field of view at the same time – it also allows for faster imaging in many cases. Examples where conventional microscopy may be more appropriate include the visualization of individual molecules, receptors or small organisms such as bacteria and yeast. Total Internal Reflection Fluorescence (or TIRF) is a powerful technique which combines the sensitivity of conventional fluorescence without out of focus light. User have access to a dedicated conventional fluorescence system equipped for time-lapse microscopy and a laser-based multi-color TIRF system (also used as the basis for the N-STORM system)

Applications:
>> Endocytosis and vesicle dynamics: High contrast, speed and sensitivity makes it possible to follow live processes close to the membrane with TIRF
>> Single molecule studies: The advanced EM-CCD camera allows the fluorescence of individual molecules to be detected even with relatively short exposures
>> Actin cytoskeleton and focal contact dynamics in live cells: Using fluorescent protein markers these processes can be followed using high time-lapse imaging
>> Yeast and bacteria studies: It is often difficult to fluorescence from microorganism by confocal microscopy without bleaching, whereas the combination of a 100x 1.49NA objective and a very sensitive camera allows fluorescence to be de-tected without need high laser power or very low exposures.
>> High speed imaging of calcium dynamics

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U28-S018. Super-resolution Microscopy (Onsite&Remote) OUTSTANDING

Fluorescence microscopy offers a wide array of tools for visualizing microscopic events. The intrinsic diffraction of light has historically made it difficult to use fluorescence to distinguish structures closer than 200nm apart. Super-resolution microscopy refers to techniques that selectively activate fluorescent molecules to map their position with up to 10 times more accuracy than conventional fluorescent microscopy. With the aim of promoting the use nanoscale imaging in biomedicine, Bionand has established one of Spain´s first N-STORM super-resolution platforms dedicated to practical applications. The Nikon N-Storm system is capable of localizing molecules with a resolution of up to 20nm.

Applications:
>> Structure determination of nanometric structures: e.g. nuclear pore receptors where individual subunits can be differentially labelled
>> High resolution neuron tracing: Super-resolution imaging has been shown to greatly improve the accuracy of neural maps given then many neural projects are below limit of conventional microscopy
>> Correlative microscopy: the higher resolution of super-resolution fluorescence makes it easier to associated with structures detected by electron microscopy
>> Single molecular studies: e.g. synapse neuroreceptor distribution
>> Nanoscale cytoskeletal and vesicle trafficking studies

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