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Posts Taged antimicrobials

Wide representation of NANBIOSIS research in NALS 2022 and best Oral Presentation to Eloi Parladé

During the last 27-29 April, the 3rd International Conference on Nanomaterials Applied to Life Sciences 2022 (NALS 2022) has taken place in the Excellence Campus of Universidad de Cantabria in Santander (Spain), organized by the University of Cantabria and Institute Valdecilla-IDIVAL.

NALS 2022 has been a multidisciplinary conference series sharing new results and ideas in the fields of biosensors, lab on a chip, drug delivery nanopharmacy. nanobiotechnology, intelligent nanomaterials, magnetic materials, nanotoxicity, antimicrobials, novel applications of 3d bioprinting and nanoimaging.

NANBIOSIS has been represented at this edition by members of several of its units, among them we must highlight the set of four oral communication presented by members of the Nanobiotechnology group-Unit 1 of NANBIOSIS “Protein Production Platform (PPP)”, from CIBER-BBN and IIB-UAB the talks were delivered by:

Eloi Parladé: “Development of ion-dependent microscale secretory granules for nanomedical applications

Carlos Martínez-Torró: “Design of a human GFP-like protein scaffold for targeted nanomedicines

Eric Voltá Durán: “Antitumoral nanoparticles with multiple activities, a close reality

Jan Atienza-Garriga: “Characterization of protein-only NPs containing amps and analysis of their protection with liposomes and micelles

They summarized a wide area of the team activities on the design of protein-based protein materials for clinical uses, produced by means of diverse types of cell factories. In particular, antimicrobial peptides, cytotoxic proteins with antitumoral targeting and drug-carrying scaffold proteins are engineered to confer self-assembling properties as either microparticles or nanoparticles, that can be further functionalized with chemical drugs through covalent binding. Microparticles are of special interest as they can be used as slow drug delivery systems for nanostructured drugs upon subcutaneous administration. Alternatively, nanoparticles can be also presented as embedded in liposomes or other micellar structures that stabilize them for enhanced performance.

Three NANBIOSIS units supported the presented research, which has been executed in a highly cooperative way: namely U1 (Protein Production Platform), led by Tony Villaverde U18 (Nanotoxicology), led by Ramón Mangues and U29 (Oligonucleotide Synthesis Platform), led by Ramón Eritja.

Among all the excellent contributions by the team, it is worthy to stress that the prize for the best Oral Presentation was granted to Dr Eloi Parladé.

Other talk by researchers from NANBIOSIS were “Antioxidant-loaded polymeric NPs prepared by nano-emulsion templating for the management of neurological diseases” by Santiago Grijalvo, from NANBIOSIS U12 and  “Exploiting GSH oxidation with nanocatalysts to promote cancer cell death” by Javier Bonet-Aletá from NANBIOSIS U9

On the other hand, Jesús Santamaría,  Scientific Director of NANBIOSIS U9 was a Keynote Speaker in the Conference with the talk: “A change of paradigm in cancer therapy? Using catalysts to make drugs inside the tumor, rather than trying systemic chemotherapy”

NALS 2022 has been an excellent conference, with presentations covering a wide range of topics in nanomaterials for health, and a great opportunity for our researchers, especially for young’s, to let know their collaborative work, as well as make new connections on common research interests, thanks to the good socializing opportunities afforded by the scheduling of the organization conference.

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In search of antimicrobials from natural bee products to coat implantable biomaterials, avoiding resistance.

The Microbial Adhesion research group-NANBIOSIS ICTS U16 Surface Characterization and Calorimetry Unit of the University of Extremadura (AM-UEX)-, belonging to the CIBER-BBN, led by Maria Luisa González, is searching in natural products, specifically in propolis, compounds with antimicrobial activity to help fight infections associated with biomaterials.

Medical devices have greatly improved healthcare. But biofilm-associated infections related to the use of these devices are a major clinical concern. Biofilms are understood as bacterial communities that adhere to the surface of the devices and are embedded in a polymeric matrix that they themselves produce. This supracellular social organization arises as a survival strategy in hostile environments, such as the human being itself, endowing the microorganisms embedded in it with resistance to mechanical clearance, the host’s immune response and antimicrobial agents. In this context, to prevent bacterial adhesion and the subsequent formation of biofilms, one of the prevention strategies is the coating of the biomaterial surfaces or the incorporation into the biomaterial itself of antimicrobial agents that can prevent their development. These type of infection are also aggravated by the multi-resistance of the microorganisms involved. For this reason, the AM-UEX group works in the search for natural products, with antimicrobial activity, that do not generate resistance, for their incorporation into new implantable biomaterials.

Bees are our allies, and their products can be a good source of available antimicrobials. Propolis is a glue for the hive and is a potentially useful food additive as it contains antioxidant and preservative properties. However, its application in other fields is limited, due to its strong flavor and low solubility. In addition, standardization is difficult because its chemical composition varies according to the flora of the environment. However, it’s common to all that they exhibit remarkable biological activities.

In a first study, the chemical composition of a Spanish propolis with a high antimicrobial capacity against bacterial strains closely related to infections associated with the formation of biofilms on biomaterials, Staphylococcus epidermidis, has been identified. The group has found in a novel Spanish ethanolic extract of propolis (SEEP) a high amount of polyphenols (205 ± 34 mg GAE / g), of which more than half correspond to the flavonoids group ( 127 ± 19 mg QE / g). The importance of this finding lies in the remarkable antioxidant and antimicrobial activities that have been attributed to this class of phenols. In addition, a more detailed analysis revealed the presence of compounds that are also present in olive oil such as vanillic acid, 1-Acetoxypinoresinol, p-HPEA-EA and 3,4-DHPEA-EDA, not previously detected in samples of propolis, which contribute to various health benefits. Other compounds found in relatively low amounts such as ferulic acid and quercetin also provide important therapeutic benefits. Regarding the antimicrobial properties of SEEP, a high sensitivity for S. epidermidis at low concentrations and a high inhibitory capacity at lower concentrations were found.

The antibacterial activity of propolis has been extensively studied, but its mechanism of action remains unclear. Research by our group has focused on measuring alterations in the physicochemical properties of the outermost surface layer of bacterial cells, both in gram-positive (S. epidermidis) and gram-negative (E. coli) cells, after incubation. with different concentrations of this antimicrobial agent. Propolis was found to induce substantial changes in bulk charge density, electrophoretic smoothness, and degree of hydrophobicity of the outermost surface layer of cells. Furthermore, observation by electron microscopy and determination of the release of cellular components carried out in NANBIOSIS Unit 16 of CIBER-BBN and UEX showed that propolis at sub-bactericidal concentrations already causes, at least locally, structural and morphological damage and/or disturbances in the cell wall. This research proposes that the mechanism of action of propolis against bacteria comes initially from the structural damage of the membrane / wall produced by the different constituents of propolis. It is a mechanism of action to which it can be difficult for bacteria to generate resistance, especially if different SEEP molecules work together synergistically.

Reference articles:

Fernández-Calderón, M. C., Navarro-Pérez, M. L., Blanco-Roca, M. T., Gómez-Navia, C., Pérez-Giraldo, C., and Vadillo-Rodríguez, V. (2020). Chemical Profile and Antibacterial Activity of a Novel Spanish Propolis with New Polyphenols also Found in Olive Oil and High Amounts of Flavonoids. Molecules 25, 3318. [DOI]

Vadillo-Rodríguez V, Cavagnola MA, Pérez-Giraldo, Fernández-Calderón MC. (2021) A physico-chemical study of the interaction of ethanolic extracts of propolis with bacterial cells. Colloids Surf B Biointerfaces 200, 111571. [DOI]

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