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U10-E39. Equipment for Analysis of scaffolds for tissue engineering – Epifluorescence microscope

Equipment funded by European Union (NextGeneratioEU) -Plan de Transfomación y Resilencia


Epifluorescence microscope with inverted optics for morphological characterization of cell samples obtained by 3D Bioprinting technique

The new Nikon Eclipse Ti2-E imaging system is based on an inverted, fully motorized, large field-of-view wide-field fluorescence microscope equipped with state-of-the-art features for high-speed visualization of large tissues or sample sets. The microscope is equipped for imaging standard slides, culture dishes, multiwells, and microplates (e.g., 96-well plates). The system includes “Perfect Focus” for applications requiring outstanding focus control (e.g., image tiling), a high-resolution motorized stage, and NIS-Elements Advanced Research (AR) software with AI-based modules for customizing sample visualization and data analysis. The heart of the system is the outstanding Kinetix camera from Photometics. The back-illuminated sCMOS sensor of the camera delivers the fastest speed (up to 500 fps at 3200×3200 pixels), and the largest field of view (25 mm of FOV) with outstanding light sensitivity, significantly improving data throughput.

The camera is coupled to a high-speed illumination system to quickly switch (<50 ms) between different wavelengths, allowing fast multi-color imaging of large samples or the quantitative determination of biological quantities using fluorescent sensitive dyes. The Ti2’s exceptionally stable, drift-free platform is designed to meet the demands of super-stable imaging. At the same time, its unique hardware-triggering capabilities enhance even the most challenging, high-speed imaging applications. Furthermore, the Ti2’s unique, intelligent functions guide users through imaging workflows by gathering data from internal sensors, eliminating the possibility of user errors. In addition, the status of each sensor is automatically recorded during acquisition, providing quality control for imaging experiments and enhancing data reproducibility in a fraction of the time of conventional equipment.

These characteristics, in combination with Nikon’s advanced Artificial Intelligence (AI) analysis capabilities integrated into the microscope’s software, make real-time analysis and optimization of the measurements possible to ensure the best results through user-specific automatic experimental routines.

Specifications and capabilities:

  • Nikon Ti2E Fully Automated Inverted Microscope
  • Motorized Stand, XY Stage, Z-Focus, Filter Turret and Nosepiece
  • Perfect Focus System
  • Real-time deconvolution and deblurring
  • Multi-well plate holder and high-content imaging software module for high-throughput imaging and data analysis
  • Tile scanning and image stitching to produce high-resolution overview images of large tissue sections
  • High-quality air and immersion objectives (CFI Plan Fluor 4×/0.13 Dry, CFI Super Plan Fluor 20×/0.70 Dry, CFI Plan Apo 25×/1.05 Silicon, CFI Plan Apo 60×/1.43 Oil)
  • 25mm Large FOV Fluorescence
  • High-speed multi-wavelength LED illumination system (CoolLED pE-4000) offering 16 (365/385/405/435/460/470/490/500/525/550/580/595/635/660/740/770 nm) wavelengths selectable across 4 channels
  • Fluorescence filter sets: DAPI (460/50), FITC (525/50), mCherry (630/75), Cy5 (690/50), and DAPI/FITC/TRITC/Cy5 multi-band
  • State-of-the-Art High Sensitivity, Large FOV sCMOS Camera
  • NIS-Elements AR High Content Packages for Scanning Plates and Creating Custom Acquisition and Analysis Routines
  • NIS-Elements 2D/3D Deconvolution Suite and NIS.ai Artificial Intelligence Module