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U6-E07. Dynamic LightScattering equipment (Zetasizer ULTRA)


Dynamic LightScattering equipment (Zetasizer ULTRA)

The Zetasizer Ultra is the world’s most capable combined DLS and ELS system, incorporating Non-Invasive Back Scatter (NIBS) and, uniquely, Multi-Angle Dynamic Light Scattering (MADLS) technology for the measurement of particle and molecular size. When it comes to those crucial measures, MADLS offers a higher resolution image of your sample’s size distribution, while NIBS offers the adaptability and sensitivity to test over a broad concentration range. The Zetasizer Ultra Red Label addon to MADLS makes it possible to analyse particle concentration directly. Particle concentration measurement is an excellent screening approach since it works well with a variety of materials, requires little to no dilution, and is easy to apply. This is a special feature of the Zetasizer Ultra that may even be used with samples that were previously exceedingly difficult to quantify, such viruses and virus-like particles (VLPs).

Technical specifications:

Particle size and molecular size

  • Measurement principle: Non-Invasive Back Scatter (NIBS) Dynamic Light Scattering. Dynamic Light Scattering (90° and 13°) Multi-Angle Dynamic Light Scattering (MADLS)
  • Measurement angle: 173º, 13º, 90º
  • Size ranges: from 0.3 nm to 10 µm.
  • Minimum volume: 3 µL
  • Minimum sample concentration:
    • Blue Label: 0.2 mg/mL 15 kDa Protein
    • Red Label: 0.1 mg/mL 15 kDa Protein
  • MADLS: 0.1 mg/ml 15 kDa Protein
  • MADLS Particle Concentration Enabled
    • Blue Label: No
    • Red Label: Yes
  • Maximum sample concentration: 40% w/v

Zeta potential

  • Measurement principle: Mixed-Mode Measurement phase analysis light scattering (M3-PALS)
  • Concentration range. Minimum sample concentration:
    • Blue Label: 10 mg/mL to 40% w/v
    • Red Label: 1 mg/mL to 40% w/v
  • Sample conductivity range: Maximum 260 mS/cm
  • Size ranges: 3.8 nm to 100 µm
  • Minimum volume: 20 µL

Compatible cells: A range of disposable and reusable cells are available to optimize the measurement in terms of sample volume and concentration, including the new low volume disposable sizing cell kit, which offers the dual benefits of size measurements in as little as 3 µL, and extension to the upper size range of DLS measurements, due to the suppression of convection currents.

 Applications: The Zetasizer Ultra is suitable for a wide range of applications, including colloidal Silica, titanium dioxide, ceramics, proteins, carbon dots (C-dots), and adeno-associated virus (AAV).