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Impact of Extracellular Vesicle Isolation Methods on Downstream miRNA Analysis in Semen

An article by Sara Larriba of the Human Molecular Genetics Group of Bellvitge Biomedical Research Institute (IDIBELL), recently published by the International Journal of Molecular Science. entitled “Impact of Extracellular Vesicle Isolation Methods on Downstream miRNA Analysis in Semen: A Comparative Study“, acknowledges NANBIOSIS ICTS for its participation in the research results. The nanoparticle tracking analysis was performed by the ICTS NANBIOSIS U6 Biomaterial Processing and Nanostructuring Unit.

Abstract:

Seminal plasma (SP) contains a unique concentration of miRNA, mostly contained in small extracellular vesicles (sEVs) such as exosomes, some of which could be clinically useful for diagnosis and/or prognosis of urogenital diseases such as prostate cancer (PCa). We optimized several exosome-EV isolation technologies for their use in semen, evaluating EV purifying effectiveness and impact on the downstream analysis of miRNAs against results from the standard ultracentrifugation (UC) method to implement the use of SP sEV_miRNAs as noninvasive biomarkers for PCa. Our results evidenced that commercial kits designed to isolate exosomes/EVs from blood or urine are mostly applicable to SP, but showed quantitative and qualitative variability between them. ExoGAG 3500× g and the miRCURY Cell/Urine/CSF 1500× g methods resulted as equivalent alternative procedures to UC for isolating exosomes/sEVs from semen for nanoparticle characteristics and quality of RNA contained in vesicles. Additionally, the expression profile of the altered semen sEV-miRNAs in PCa varies depending on the EV isolation method applied. This is possibly due to different extraction techniques yielding different proportions of sEV subtypes. This is evidence that the exosome-EV isolation method has a significant impact on the analysis of the miRNAs contained within, with important consequences for their use as clinical biomarkers. Therefore, miRNA analysis results for EVs cannot be directly extrapolated between different EV isolation methods until clear markers for delineation between microvesicles and exosomes are established. However, EV extraction methodology affects combined models (semen exosome miRNA signatures plus blood Prostate specific antigen (PSA) concentration for PCa diagnosis) less; specifically our previously described (miR-142-3p + miR-142-5p + miR-223-3p + PSA) model functions as molecular marker from EVs from any of the three isolation methods, potentially improving the efficiency of PSA PCa diagnosis..

Article:

Impact of Extracellular Vesicle Isolation Methods on Downstream miRNA Analysis in Semen: A Comparative Study. Marina Mercadal, Carolina Herrero, Olga López-Rodrigo, Manel Castells, Alexandre de la Fuente, Francesc Vigués, Lluís Bassas and Sara Larriba. Int. J. Mol. Sci. 2020, 21, 5949; doi:10.3390/ijms21175949

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Nanoprobes for the next generation of Optical Imaging

Judit Morlà Folch, CIBER-BBN Researcher in Nanomol Group and NANBIOSIS U6 Biomaterial Processing and Nanostructuring Unit at ICMAB-CSIC is giving the seminar “Nanoprobes for the next generation of Optical Imaging“, today Monday, 5 October 2020 at 12 pm, Hosted by Nora Ventosa, Nanomol Group in the cycle of Online ICMAB PostDoc Talk.

Online Invited Seminar by Zoom. Register here to attend.

About the Seminar: Molecular imaging plays a vital role in the healthcare sector, since abnormal conditions and diseases are often diagnosed through imaging, while therapeutics methods used for the treatment of the abnormalities are often guided by imaging. Optical imaging is a highly sensitive technique and low-cost procedure in comparison to tomography, Magnetic Resonance Imaging (MRI) or ultrasounds among the other forms of imaging currently employed.  Among the imaging agents, fluorescent organic nanoparticles (FONs) are emerging as an attractive alternative to the well-established fluorescent inorganic nanoparticles or small organic dyes. Their proper design allows one to obtain biocompatible probes with superior brightness and high photostability, although usually affected by low colloidal stability

Judit Morlà Folch began her postdoc in 2008 at New Jersey Institute of Technology (NJIT, EEUU) under the Marie Curie Tecniospring and Postdoctoral Programme in collaboration with ICMAB-CSIC (Nanomol Group), where she is nowadays working on the development of fluorescent organic nanoparticles for bioimaging. 

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New updated version of the CSIC report on COVID-19

The report by Spanish Higher Council for Scientific Research (CSIC) summarizng the most relevant scientific results on the Covid-19 pandemic.  “A global vision of the Covid-19 pandemic: What we know and what we are investigating from CSIC”, has just published its version number 4.

This report is structured in five blocks: prevention, disease, containment and diagnosis, treatment and vaccines, and global impact.

The containment and diagnosis block describes how the virus is transmitted in the air indoors and the analysis of wastewater as a system for early detection of SARS-CoV-2 is explained. In addition, the importance of diagnosis in the face of the pandemic is underlined and the different types of detection tests available and the diagnostic strategies to control the spread of the infection are detailed. The CIBER-BBN/IQAC-CSIC Nb4D research group and its research platform NANBIOSIS-ICTS Unit 2 Custom Antibody Service (CAbS) takes and important role in this block.

Pilar Marco, Scientific Director of CAbS is co-author, toguether with Cesar Fernandez (IBM-CNM-CSIC) of the chapter 3.4 “The role of the Diagnosis in the face of pandemic” wich provides information on the types of tests, how to perform and how to use them for the different purposes for which  diagnosis is an indispensable tool. “From a broad perspective, we can  distinguish between two types of tests: A. Viral tests: they detect the SARS-CoV-2 virus, include the well-known PCR (Polymerase Chain Reaction) or molecular tests, which detect the genetic material virus B. Test that detect the response of the human body to infection. They include so-called “serological tests, which detect antibodies produced by the patient’s immune system, although other types of biomarkers can also be detected.”

For further information and access to report: http://hdl.handle.net/10261/218312 

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XIV COURSE OF LAPAROSCOPIC SURGERY OF COLON

CCMIJU has organised the XIV Course of Laparoscopic Surgery of Colon, which wil take place at JUMISC from 14 December to 16 December 2020 under the Direction Francisco Miguel Sánchez Margallo (Deputy Scientific Director of NANBIOSIS)

Two of the main lines in the activity of the Minimally Invasive Surgery Centre (CCMIJU) are the Innovation and medical training in collaboration with hospitals and companies of the medical sector, with the aim to improve the quality of healthcare delivered to patients, by providing
surgeons and other health professionals with innovative solutions and
training. The CCMIJU is part of the Singular Scientific-Technical Facility
(ICTS) NANBIOSIS, featuring state-of-the-art technology to be
used during the training courses.

Frurther information: Programm and inscriptions

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