The purification of the antibodies includes the isolation of the antibody from the serum (polyclonal antibodies) or culture supernatant from hybridoma cell lines (monoclonal antibodies). The services offers previous advise on the different possibilities for purification according to the needs of the user.
The methods of purification that we offer vary from crude methods (precipitation of the proteins from the sample including any antibody present) to general purification (purification by affinity of certain classes of antibodies without taking account of the specificity to the antigen) to specific (purification by affinity of the antibodies which join specifically to a definite antigen).
- Ammonium sulfate precipitation
- Protein A, protein G affinity purification
- Specific antigen affinity purification