08 Feb12:20pm
Nanbiosis
Freeze Substitution (FS) of specimens in methanol, acetone or any other FS media at low temperatures is the next step following high pressure freezing and other cryo-fixation methods.
Progressive Lowering of Temperature (PLT) allows substitution and resin infiltration of chemically fixed specimens. Finally, the sample is polymerized under UV light in the EM AFS2 and can be cut and immunolabelled.
Applications:
Fundamental research in cellular and structural biology, and studies involving soft materials that cannot be resin-embedded due to their composition.