U2. Custom Antibody Service (CAbS)
- Scientific Director: Prof. Pilar Marco firstname.lastname@example.org
- Scientific Coordinator: Dra. Nuria Pascual email@example.com
- Entities: Institute of Avanced Chemistry of Catalonia (IQAC) & Consejo Superior de Investigaciones Científicas (CSIC)
- Address: Jordi Girona, 18-26, 088034, Barcelona, Spain
- Phone: +34 934 006 100
- Web: IQAC- CSIC
The facility is located in the Institute of Advanced Chemistry of Catalonia (IQAC-CSIC; Barcelona). It is equipped with a laboratory for the development and production of monoclonal antibodies. The laboratory is fully equipped for obtaining, selecting and storing monoclonal antibody producing hybridomas. In addition, the facility has infrastructure for the production of polyclonal antibodies, as well as laboratories for the synthesis of bioconjugates, haptens, the purification of antibodies and the performance of immunoassays. In addition to the above mentioned equipment, the IQAC-CSIC provides animal husbandry facilities and a unit for the synthesis of organic molecules.
The equipment consists in an inverted phase contrast microscope with a camera, two Class II laminar flow biological safety cabinets, two CO2 incubators, a bioreactor for hybridoma culture, and tangential filtration unit for purification of the antibodies produced in the culture. Among the items of available equipment it should be highlighted a spotter for selection of high antibody producing cell lines and an automated ELISA microplate washer an ELISA reader, two AKTA Prime Liquid chromatography systems for antibody purification, plus standard equipment (centrifuges, fridges, liquid nitrogen containers, autoclave, baths, and vacuum equipment) and all the necessary routine laboratory apparatus.Cabs also has access to a fully equipped chemistry laboratory for the synthesis of haptens and immunoreagents.
- U2-S01. Scientific and technical support (Remote)
- U2-S02. Hapten synthesis (Remote) OUTSTANDING
- U2-S03. Preparation of bioconjugates and molecular probes (Remote) OUTSTANDING
- U2-S04. Polyclonal Antibody Production (Remote) OUTSTANDING
- U2-S05. Monoclonal Antibody development (Remote) OUTSTANDING
- U2-S06. Hybridoma cell culture antibody production (Remote) OUTSTANDING
- U2-S07. Antibody purification (Remote) OUTSTANDING
- U2-S08. Inmunochemical methods development (Remote) OUTSTANDING
FOR THOSE SERVICES IDENTIFIED AS OUTSTANDING, AT LEAST 20% OF THEIR CAPACITY IS OPEN UNDER COMPETITIVE ACCESS. SEE ANNEX 1 OF ACCESS PROTOCOL FOR DETAILS ON % OF OPENNESS FOR EACH SERVICE
- U2-E01. Cell culture laboratory for hybridoma production.
- U2-E02. HiClass II Type A2 Biological Safety Cabinet (2 units)
- U2-E03. Inverted Microscope Leica DM IL LED with CCD camera
- U2-E04. CO2 cell culture incubator with UV contamination control (2 units)
- U2-E05. AKTA Prime FPLC system for purification of proteins
- U2-E06 Tangential Flow Filtration System (KrosFlo® Research Iii)
- U2-E07. Laboratory fully equipped for immunochemical methods.
- U2-E08. Laboratory perfectly equipped for chemical synthesis (to hapten synthesis)
- U2-E09. Animal facility.
|Ref||Title||Funding Organism||Unit Role|
|SAF2015-67476-R||Immunochemical Strategies of Diagnosis and Therapy based on Quorum Sensing (ImmunoQS)||Ministerio de Economía y Competitividad||Participant|
|PIE1400061||Molecular links between diabetes and neurodegenerative diseases||ISCIII||Oberver|
|2014SGR1484||Consolidated Research Group||Generalitat de Catalunya||Participant|
|FP7-614168||Real time monitoring of SEA contaminants by an autonomous Lab-on-a-Chip biosensor (SEA-on-a-CHIP)||European Commission||Oberver|
|CI16-00031 Caixa Impulse||A new approach for the detection of Pseudomonas aeruginosa||CaixaBank||Participant|
|MSCA-ITN2015-675412||New Diagnostics for Infectious Diseases||European Commission||Participant|
|TECCTP15-1-0011||Ajuda acreditació Tecnio del grup Nb4D||ACCIÓ: Generalitat de Catalunya||Others|
- Roncador G., Engel P., Maestre L., Anderson A.P., Cordell J.L., Cragg M.S. et al. The European antibody network’s practical guide to finding and validating suitable antibodies for research. mAbs. 2016;8(1):27-36.
- Pastells C., Pascual N., Sanchez-Baeza F., Marco M.-P.. Immunochemical Determination of Pyocyanin and 1-Hydroxyphenazine as Potential Biomarkers of Pseudomonas aeruginosa Infections. Analytical Chemistry. 2016;88(3):1631-1638.
- Salvador J.-P., Marco M.-P.. Amperometric Biosensor for Continuous Monitoring Irgarol 1051 in Sea Water. Electroanalysis. 2016;28(8):1833-1838.
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