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U1. Protein Production Platform Unit (PPP)

U1. Protein Production Platform (PPP)

U1. PPP logo IBB UAB

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The PPP Unit is at the Institute of Biotechnology and Biomedicine of the Autonomous University of Barcelona (IBB-UAB). It counts with the necessary facilities for the design, production, and purification of recombinant proteins. This facility is coordinated by the Nanobiotechnology Group, led by Prof. Antonio Villaverde. It has both highly specialized personnel and the necessary equipment to offer a “tailored” service for the design, production, and purification of recombinant proteins using both prokaryotic and eukaryotic expression systems. Its location allows this service to be linked with other complementary services available within the university (Cell Culture, Cytometry, Production of Antibodies, Microscopy, Proteomic, and Bioinformatic, X-ray crystallography, and Microarray and Sequencing Services), as well as facilitating the management of subsequent uses of the produced protein.

Counts with a laboratory perfectly equipped for the bioproduction of proteins, another with all the necessary equipment for molecular cloning, plus one lab for the purification and characterization, and finally one for cryopreservation.

The PPP Unit has implemented the ISO9001 certification for standard quality control system.

IQNet ISO 9001:2015 Certification PPP

AENOR ISO 9001:2015 Certification PPP

Active projects

Title Fundin: Organism Call: Funding source Role
GA: 720942 SMART FUNCTIONAL GLA-NANOFORMULATION FOR FABRY DISEASE – Smart-4-Fabry Unión Europea (Comisión Europea) H2020-NMBP-2016-2017 Coordinator
SAF2017-90810-REDI Strategic Promotion and coordinated management of Nanbiosis: Pronanbiosis II Agencia Estatal de Investigación (AEI) Acciones de dinamización «REDES DE EXCELENCIA»
-ICTS 2017

Other projects

Ref Title Funding Organism Unit Role
PI15/00272 Design of intelligent nanoconjugates for the treatment of metastatic colorectal cancer. Instituto de Salud Carlos III Participant
2014SGR-132 Basic and Applied Microbiology AGAUR Participant
RTC-2014-2207-1 TERARMET: Development of therapies for the treatment of rare congenital metabolic diseases MINECO Participant
Marato 416/C/2030TV32013-132031 Genotoxic nanoparticles targeting colorectal cancer stem cells Marato TV3 Participant



  • Corchero J.L. Eukaryotic aggresomes: from a model of conformational diseases to an emerging type of immobilized biocatalyzers. Applied Microbiology and Biotechnology. 2016;100(2):559-569.
  • Saccardo P., Corchero J.L., Ferrer-Miralles N.. Tools to cope with difficult-to-express proteins. Applied Microbiology and Biotechnology. 2016;:1-9.
  • Torrealba D., Seras-Franzoso J., Mamat U., Wilke K., Villaverde A., Roher N. et al. Complex particulate biomaterials as immunostimulant-delivery platforms. PLoS ONE. 2016;11(10)
  • Cano-Garrido O., Sanchez-Chardi A., Pares S., Giro I., Tatkiewicz W.I., Ferrer-Miralles N. et al. Functional protein-based nanomaterial produced in microorganisms recognized as safe: A new platform for biotechnology. Acta Biomaterialia. 2016;43:230-239.
  • Serna N., Cespedes M.V., Saccardo P., Xu Z., Unzueta U., Alamo P. et al. Rational engineering of single-chain polypeptides into protein-only, BBB-targeted nanoparticles. Nanomedicine: Nanotechnology, Biology, and Medicine. 2016;12(5):1241-1251.
  • Rueda F., Gasser B., Sanchez-Chardi A., Roldan M., Villegas S., Puxbaum V. et al. Functional inclusion bodies are produced in the yeast Pichia pastoris. Microbial Cell Factories. 2016;15(1)
  • Cabrera I., Abasolo I., Corchero J.L., Elizondo E., Gil P.R., Moreno E. et al. α-Galactosidase-A Loaded-Nanoliposomes with Enhanced Enzymatic Activity and Intracellular Penetration. Advanced Healthcare Materials. 2016;5(7):829-840.
  • Céspedes MV, Unzueta U, Álamo P, Gallardo A, Sala R, Casanova I et al. Cancer-specific uptake of a liganded protein nanocarrier targeting aggressive CXCR4+ colorectal cancer models.Nanomedicine : nanotechnology, biology, and medicine. 2016;12(7)
  • Giannotti M.I., Abasolo I., Oliva M., Andrade F., Garcia-Aranda N., Melgarejo M. et al. Highly Versatile Polyelectrolyte Complexes for Improving the Enzyme Replacement Therapy of Lysosomal Storage Disorders. ACS Applied Materials and Interfaces. 2016;8(39):25741-25752.

News U1

27 Dec

NANBIOSIS U1_Protein Production Platform expands its facilities at the Autonomous University of Barcelona, strengthening its capabilities.

NANBIOSIS Unit 1 Protein Production Platform (PPP) of CIBER-BBN and UAB, has taken a significant step towards enhancing its service capabilities in the field of recombinant protein production and purification. Until now, due to space constraints at the Institute of Biotechnology and Biomedicine (IBB), U1’s activities were confined to half of a small laboratory. However, thanks to the ongoing commitment of the center to bolster the PPP’s activities, this unit has gained access to a laboratory, along with an office, exclusively designated for the platform within the IBB premises, creating an optimal environment for the process of protein production and[...]

20 Dec

Good News! Protein Nanoparticles with a New Ligand Select and Destroy Tumor-associated Fibroblasts”

With the participation of two units of NANBIOSIS ICTS and the expertise of the scientists managing these units The study, fruit of the collaboration between the Nanotechnology group of the Institute of Biotechnology and Biomedicine (IBB-UAB), led by Prof. Antonio Villaverde, and the Oncogenesis and Antitumor Drugs group of the Sant Pau Research Institute, led by Dr. Ramon Mangues, both members of CIBER-BBN, has made significant progress by identifying the natural ligand PDGFD as an effective tool to target protein nanoparticles to tumor-associated fibroblasts that overexpress the PDGFR-β receptor. Given the relevance of the discovery, this technology has been intellectually[...]

30 Nov

NANBIOSIS U1 PPP invited to PEGS, the Protein & Antibody Engineering Summit

*Image explained by Merce Márquez Martínez, Coordinator of NANBIOSIS U1 The NBT group and Unit 1 of NANBIOSIS (Protein Production Platform, PPP), from CIBER-BBN and IBB-UAB, participated in the 15th PEGS Europe conference held in Lisbon from November 14th to 16th. PEGS, or the Protein & Antibody Engineering Summit, is a prestigious conference, organized by the Cambridge Healthtech Institute (CHI), focusing on protein engineering and its applications in drug discovery, development, and delivery. This conference serves as a valuable platform for knowledge exchange among researchers, scientists, industry experts, and professionals in the field of protein science. From left to right,[...]

08 Nov

Impactful research with NANBIOSIS participation in the Poster Tour of CIBER-BBN & CIBEREHD Annual Conference.

2023 CIBER-BBN Annual meeting has taken place at Santemar Hotel, in Santander during November 6-7. This year the format of our annual conferences has been changed towards a collective event scheme between the CIBER-BBN and CIBEREHD thematic areas. On Monday 6 the scientific sessions werecommon for EHD and BBN, with appealing contents for the mixed audience. On Tuesday 7 EHD and BBN sessions will specific for each area in separate rooms (with common coffee break). Posters of both areas were on display in the exhibit hall throughout the entirety of the Annual Meeting. Moreover, at the “Posters & beers” session (Monday[...]

23 Oct

NANBIOSIS U1 at UAB Innovation Fair

On October 17, 2023, the NANBIOSIS U1 of Protein Production Platform PPP, took part in the UAB Innovation Fair, where various research groups, spin-offs, service providers, research centers, and companies gathered to foster networking opportunities. This event proved highly advantageous for the PPP, as it not only facilitated the identification of potential new clients but also paved the way for collaborative efforts aimed at enhancing and refining the final product offered by the PPP. PPP NANBIOSIS U1 Scientists at PPP stand at the Fair: Neus Ferrer, Scientific Director; Rosa Mendoza, Laboratory Manager, and Mercedes Marquez, Scientific Coordinator. The UAB Innovation[...]

28 Sep

Nanoligent raised €2.8 M and completed Seed round financing with support of i&i Biotech Fund

September 27th 2022 – Nanoligent SL, a Spanish biotech company specialized in the development of cancer treatments based on unique protein conjugates, today announces the completion of a Seed financing round of total € 2.8 M. The final closing of the Seed round consists of € 1 M investment by i&i Biotech Fund I (i&i Bio), an early-stage Life Science fund with teams in Luxemburg and Prague which is backed by the European Investment Fund. i&i Bio joined previous Nanoligent investors, Italian Angels for Growth, the largest network of business angels in Italy, and AVANTECA Partners, a Swiss privately held[...]

16 Aug

Two NANBIOSIS Units of CIBER-BBN at UAB, one of the 300 best universities in the world, according the Shanghai Ranking

The Academic Ranking of World Universities (ARWU), known as Shanghai Ranking, which was made public on August 15, places the Autonomous University of Barcelona among the elite of the 300 best universities in the world. This indicator organizes up to 20,000 university centers worldwide,based on transparent methodology and objective third-party data. ARWU is regarded as one of the three most influential and widely observed university rankings Two of NANBIOSIS Units created by UAB and CIBER-BBN are part of this university: U1 Protein Production Platform (PPP), led by Toni Villaverde, Neus Ferrer and Mercedes Marquez, offer an “tailored” service for the design,[...]

25 Jun

How engineered protein helps Nanomedicine againts Cancer

The use of protein nanoparticles as biomaterials have been rising in recent years due to their characteristics: high biocompatibility, structural versatility, biodegradability and plasticity of design. We can later incorporate peptide ligands for specific targeting as fusion proteins and use these nanoparticles for targeted nanomedicine. However, not all proteins can be used as scaffolds for targeted drug delivery, as they need to meet certain criteria. First, it is crucial that the proteins used as a scaffold allow site-specific drug conjugation. The stability and proteolysis resistance of these proteins is also important to remain assembled during the bloodstream circulation. In addition,[...]

U1-Protein Production Platform - PPP
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U27. High Performance Computing

Ciber bbn

U27. High Performance Computing

  • Scientific Director: Prof. Pablo Laguna laguna@unizar.es
  • Scientific Coordinator: Dariel Figueredo dfigueredo@unizar.es
  • Entities: I3A-Universidad de Zaragoza.
  • Address: C/Mariano Esquillor s/n 50018, Zaragoza
  • Phone: +34 976 76 27 07
  • Web: I3A-UZ

Nanabiosis-logo Universidad de Zarargoza-U9-U13-U27.

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Located at the Aragon Institute of Engineering Research (I3A), an Interdisciplinary University Research Institute specialized in engineering of the University of Zaragoza, this HPC service counts with a conditioned room to host a cluster of computers that allows remote access for High Performance Computing, massive storage and different software applications for the research in four divisions: Biomedical Engineering, Information and Communications Technologies Division, Processes and Recycling Division and Industrial Technology Division. This Unit is coordinated by Prof. Pablo Laguna, PI of the Biomedical Signal Interpretation & Computational Simulation Groups (BSICoS).

Active projects

Title Fundin: Organism Call: Funding source Role
SAF2017-90810-REDI Strategic Promotion and coordinated management of Nanbiosis: Pronanbiosis II Agencia Estatal de Investigación (AEI) Acciones de dinamización «REDES DE EXCELENCIA» -ICTS 2017 Partner
PICCOLO-732111 Multimodal highly-sensitive PhotonICs endoscope for improve in vivo COLOn Cancer diagnosis and clinical decision support European Commission-H2020 H2020-ICT-2016-1 Partner
0624_2IQBIONEURO_6_E Promotion of an R&I network in biological chemistry for the diagnosis and treatment of neurological diseases European Union Interreg : European Territorial Co-operation POCTEP Interreg España-Portugal Partner

Other projects

Ref Title Funding Organism Unit Role
UZCUD2016-TEC-03 Optimization of a submersible device for the monitoring of the photoplestimográfica signal CUD Participant



  • Laguna P., Martinez Cortes J.P., Pueyo E.. Techniques for Ventricular Repolarization Instability Assessment from the ECG. Proceedings of the IEEE. 2016;104(2):392-415.
  • Pueyo E., Dangerfield C.E., Britton O.J., Virag L., Kistamas K., Szentandrassy N. et al. Experimentally-based computational investigation into beat-to-beat variability in ventricular repolarization and its response to ionic current inhibition. PLoS ONE. 2016;11(3):-.
  • Romero D., Martinez J.P., Laguna P., Pueyo E.. Ischemia detection from morphological QRS angle changes. Physiological Measurement. 2016;37(7):1004-1023.
  • Vicente J., Laguna P., Bartra A., Bailon R.. Drowsiness detection using heart rate variability. Medical and Biological Engineering and Computing. 2016;:1-11.
  • Alcaine A., De Groot N.M.S., Laguna P., Martinez J.P., Houben R.P.M.. Spatiotemporal model-based estimation of high-density atrial fibrillation activation maps. Digital Signal Processing: A Review Journal. 2016;54:64-74.
  • Bolea J., Pueyo E., Orini M., Bailon R.. Influence of heart rate in non-linear HRV indices as a sampling rate effect evaluated on supine and standing. Frontiers in Physiology. 2016;7(NOV):-.
  • Ortigosa N., Rodriguez-Lopez M., Bailon R., Sarvari S.I., Sitges M., Gratacos E. et al. Heart morphology differences induced by intrauterine growth restriction and preterm birth measured on the ECG at preadolescent age. Journal of Electrocardiology. 2016;:-.
  • Hernando A., Lazaro J., Gil E., Arza A., Garzon J.M., Lopez-Anton R. et al. Inclusion of Respiratory Frequency Information in Heart Rate Variability Analysis for Stress Assessment. IEEE Journal of Biomedical and Health Informatics. 2016;20(4):1016-1025.

News U27

11 Nov

Researchers of two NANBIOSIS units success in the Third Millennium Awards

Researchers of two NANBIOSIS units success in the Third Millennium Awards: the Young Research Talent award to Julia Ramirez (NANBIOSIS U27) and the Research and Future Award to the NFP group (NANBIOSIS U9) Last November 8, four initiatives received the highest award in the eighth edition of the HERALDO contest Third Millennium Awards which represent the recognition of the Aragonese community from the youngest to the most consolidated trajectories in knowledge transfer, innovation, and scientific dissemination. The Paraninfo of the University of Zaragoza hosted this event in which researchers CIBER-BBN – NANBIOSIS were recognised this year: The Films and Nanostructured[...]

02 Mar

Conversations with women scientists: Cardiology

Esther Pueyo, member of the BSICoS group, which coordinates NANBIOSIS U27 “High Performance Computing” from CIBER-BBN and I3A-UZ, participes with Lina Badimon, professor at the CSIC and group leader at the CIBERCV in the first of a series of videos where two women scientists share their experiences, prepared by the Scientific Culture and Innovation Unit (UCC+i) of the CIBER Both researchers work in the field of Cardiology and share their passion for science and their vocation to help patients. In this video they talk in depth about their career, their difficulties, their achievements, and what their vision is about t[...]

08 Feb

Women in Signal Processing: Raquel Bailón

The International Day of Women and Girls in Science on February 11 honor women’s significant achievements in science and place a much-needed focus on girls entering Science, Technology, Engineering, and Mathematics (STEM) careers. We want to take this day to congratulate all the women scientists, especially to our scientists at NANBIOSIS ICTS. In this occasion we want to put the spotlight on Raquel Bailon, researcher of Bsicos Group coordinating NANBIOSIS Unit 27 of High-Performance Computing form CIBER-BBN and University of Zaragoza-I3A, who last month has been highlighted by Inside Signal Processing Newsletter. In the interview Dr. Raquel Bailon talks about her motivations[...]

15 Oct

Pablo Laguna talking about Physiologically driven biomedical signal processing at the Autonomous University of San Luis Potosí

Zaragoza, October 14th, 2021 Bsicos.i3a.es Pablo Laguna, Scientific Director of NANBIOSIS U27 High Performance Computing and researcher of BSICoS Group of I3A-UNIZAR and CIBER-BBN gave a talk about Physiologically driven biomedical signal processing at the Faculty of Physics at the Autonomous University of San Luis Potosí (UASLP) in Mexico. He explaned how biomedical signals convey information about biological systems and can emanate from sources of as varied origins as electrical, mechanical or chemical. In particular, biomedical signals can provide relevant information on the function of the human body. This information, however, may not be apparent in the signal due to measurement noise,[...]

24 Sep

Cristina Pérez, Young Investigator Award at the STAFF congress

Cristina Pérez, has been the winner of the Young Researcher Award (YIA) at the STAFF congress held in Sirolo (Italy), from Septembre 1 to 4, for her work entitled “Characterization of impaired ventricular repolarization by quantification of QT delay after heart rate changes in stress test” Cristina Pérez is a researcher from BSICoS. Research Group that coordinates NANBIOSIS ICTS Unit 27 “High Performance Computing”, led by PAblo Laguna (from I3A-UNIZAR and CIBER-BBN) Further information at BSICoS Websit[...]

24 Sep

Diego García, finalist in the Young Researcher Award at the CinC

Researchers from BSICoS Research Group – NANBIOSIS U27 (from I3A-UNIZAR and CIBER-BBN presented their works in the Computing in Cardiology (CinC) Conference, held in Brno (Czech Republic) on 12-15 September. Diego García was Young Researcher Award (YIA) finalist with the work “Ventilatory Thresholds Estimation Based on ECG-derived Respiratory Rate”. The purpose of this work is to study the feasibility of estimating the first and second ventilatory thresholds (VT1 and VT2, respectively) by using electrocardiogram (ECG)-derived respiratory rate during exercise testing. The computation was performed by the ICTS NANBIOSIS, specifically by the High Performance Computing Unit of the CIBER-BBN at the I3A-University of[...]

18 Mar

The relevance of biomedical signal processing in the understanding of biological systems

Within the framework of NeoCom2021 Jesús Lázaro, researcher of BSICoS group and NANBIOSIS U27 High Performance Computing form CIBER-BBN and I3A-UZ will explain how biomedical signal processing can be used to improve the current understanding of the functioning of biological systems, conditions related to the cardiovascular, respiratory, and autonomic nervous systems, as well as their interactions. Prof. Lázaro will review the progress of the WECARMON European Project whose objective is the development of a system for long-term monitoring (months / years) of patients with cardiorespiratory diseases. NEOCOM: As every year, the Territorial Demarcation of the COIT in Aragon and t[...]

25 Jan

Electrocardiogram-Derived Tidal Volume During Treadmill Stress Test

Researchers of BSICoS Group and NANBIOSIS ICTS U27 High Performance Computing from CIBER-BBN and I3A-UZ have published a new article in the scientific journal IEEE Transactions Biomedical Engineering. proposing a new method to estimate tidal volume during stress test based only on the electrocardiogram signal. Electrocardiogram (ECG) has been regarded as a source of respiratory information with the main focus in the estimation of the respiratory rate. Although little research concerning the estimation of tidal volume (TV) has been conducted, there are several ECG-derived features that have been related with TV in the literature, such as ECG-derived respiration, heart rate variability or respiratory rate. In this work, resarchers[...]

U27- High Performance Computing
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