Nanbiosis

U2. Custom Antibody Service (CAbS)

• Scientific Director: Prof. Pilar Marco pilar.marco@cid.csic.es
• Scientific Coordinator: Dra. Nuria Pascual npdqob@cid.csic.es
• Entities: Institute of Avanced Chemistry of Catalonia (IQAC) & Consejo Superior de Investigaciones Científicas (CSIC)
• Address: Jordi Girona, 18-26, 088034, Barcelona, Spain
• Phone: +34 934 006 100
• Web: IQAC- CSIC

U2 CAbS-Monoclonal antibody development

U2 CAbS-Hapten synthesis

U2 CAbS-Antibody purification

Description

The facility is located in the Institute of Advanced Chemistry of Catalonia (IQAC-CSIC; Barcelona). It is equipped with a laboratory for the development and production of monoclonal antibodies. The laboratory is fully equipped for obtaining, selecting and storing monoclonal antibody producing hybridomas. In addition, the facility has infrastructure for the production of polyclonal antibodies, as well as laboratories for the synthesis of bioconjugates, haptens, the purification of antibodies and the performance of immunoassays. In addition to the above mentioned equipment, the IQAC-CSIC provides animal husbandry facilities and a unit for the synthesis of organic molecules.

The equipment consists in an inverted phase contrast microscope with a camera, two Class II laminar flow biological safety cabinets, two CO2 incubators, a bioreactor for hybridoma culture, and tangential filtration unit for purification of the antibodies produced in the culture. Among the items of available equipment it should be highlighted a spotter for selection of high antibody producing cell lines and an automated ELISA microplate washer an ELISA reader, two AKTA Prime Liquid chromatography systems for antibody purification, plus standard equipment (centrifuges, fridges, liquid nitrogen containers, autoclave, baths, and vacuum equipment) and all the necessary routine laboratory apparatus.Cabs also has access to a fully equipped chemistry laboratory for the synthesis of haptens and immunoreagents.

News U2

28 Dec

New results evidence new biomarkers for early diagnosis of P. aeruginosa infections

Pseudomonas aeruginosa is a common multidrug-resistant pathogen that causes acute and chronic infections. However, P. aeruginosa, as many other bacterial species, has developed resistance to antibiotics being difficult to treat. For this reason diagnostic methods allowing detection at early  stages of the infection are required  and, therefore, efficient biomarkers of infection are very helpful. These fast diagnosis will help on the subsequent therapeutic treatment. The Nb4D group of CIBER-BBN and IQAC-CSIC (led by M.-Pilar Marco) has recently conducted a research to develop a highly sensitive, specific and reliable immunochemical assay to detect pyocyanin (PYO), one of the most important virulence factors[...]

01 Dec

Nanomedicine in the Medicine of the Future

Scientists of CIBER-BBN and NANBIOSIS ICTS have participated in the 4th Conference “Anticipating the Medicine of the Future”, which took place on November 30th, organized by the Roche Institute Foundation. The topics for this ediction had been identified by the Observatory of Trends in the Medicine of the Future: Pharmacogenomics, Nanomedicine and Epigenomic The event counted with three roundtables for discussion in relation to the three topics. The second one, on Nanomedicine, was moderated by Joaquín Arenas, Director of the Research Institute of the 12 de Octubre University Hospital. Ramón Martínez Máñez, Professor of Inorganic Chemistry at the Interuniversity Researc[...]

22 Nov

‘Point-of-care or PoC’ devices are able to directly detect the genetic material of the virus in just 30 minutes

Twenty five Spanish researchers linked to the Higher Center for Scientific Research, CSIC, who are working more directly on the project ‘Point-of-care tests for the rapid detection of SARS-CoV-2’ – Studies for the development of technologies effective diagnosis and early detection of SARS-CoV-2- have met since last Wednesday and until this Friday at the Benasque Science Center ‘Pedro Pascual’ to analyze the state or of the different programs and lines of research on the covid-19. After months of intense work, among the most outstanding achievements in relation to the detection of the genetic material of the virus, is the development[...]

02 Nov

Nanobodies for biosening at the European Biosensor Symposium digital seminars

Next November 8, the researcher of NANBIOSIS U2 Custom Antibody Service (CAbS)  J.-Pablo Salvador will host the Seminar “Nanobodies for biosensing” in the framework of European Biosensor Symposium digital seminar series which are schedulled on the third Tuesday of every month. Nanobodies® (Nbs) are the recombinant binding domain from the heavy chain antibodies tipically produced from camèlids.  Besides their great potential as molecules in drug development, Nanobodies possess excellent functional properties that aid in their development for diagnostic tools. In this seminar, Dr. Salvador will explain the the outstanding properties of Nanobodies. Three graduate student speaker and up to five graduat[...]

23 Sep

High quality antibodies for very challenging projects

Researchers of Nb4D group – Unit 2 of the ICTS NANBIOSIS (led by Pilar Marco, from CIBER-BBN and the IQAC-CSIC) have participated in Expoquimia 2021, the International Chemistry Meeting, which took place from 14 to September 17 at the Fira de Barcelona.The researchers presented their work in several projects: the FoodSmartPhone project (MSCA-ITN-720325 action), the QS4CF (RTI2018-096278-B-C21), the QS-Motion (TV32018-201825-30-31) and on the PoC4CoV project (PIE-202050E090; PTI Salud Global, CSIC). Research at the FoodSmartPhone(MSCA-ITN-720325 action) is focus on developing devices for the monitoring of contaminants of interest in food safety through the use of mobile phones. The production of t[...]

20 Sep

The European Researcher’s Night with NANBIOSIS’ scientists.

The European Researchers’s Nigth is a public event dedicated to the dissemination of science that is held every year in more than 300 cities in 30 countries of Europe at the same time, organized on the last Friday and Saturday of September. This year face-to-face activities are progrmmed in different locations of Catalonian cities and also the “Nit de la Recerca” YouTube channel offers more than 20 noves microxerrades led by researchers from Catalonia. Two NANBIOSIS’ researchers have joined this event to explain their research: Julian Guercetti – Nb4D Group – NANBIOSIS U2 CAbS, from IQAC-CSIC and CIBER-BBN, will giv[...]

15 Sep

European Biosensor Symposium digital seminars, co-organized by Pilar Marco

Pilar Marco, the Scientific Director of NANBIOSIS U2 , Custom Antibody Service (CAbS), and leader of the group Nanobiotechnology for Diagnostics (Nb4D) from CIBER-BBN and IQAC-CSIC, coorganises the European Biosensor Symposium digital seminar series. The aim of these seminar series is to continue with the purpose of the European Biosensor Symposium, held in March 2021, to allow especially juior reseachers to present their research, exchange ideas and built networds. The seminars are scheduled on the third Tuesday of every month at 18:00 CET, via zoom. Each of the seminars is organized by a host focussing on a specific topic: “Interferometric sensors“, hosted[...]

29 Apr

Five experts explain the results of the CSIC Global Health Platform after a year of research on the SARS-CoV-2 coronavirus

– Margarita del Val, Pilar Marco, Mario Mellado, Diego Ramiro and Iñaki Comas will disseminate, in a webinar through YouTube, the works on the disease, diagnosis and social impact– The platform investigates the new coronavirus in six areas: prevention, disease, containment and diagnosis, treatment and vaccines, social impact and communication Five experts from the Higher Council for Scientific Research (CSIC) will explain in a webinar broadcast on YouTube, on Wednesday, May 5 at 6:00 p.m., the results of a year of research at the CSIC on the SARS-CoV-2 coronavirus carried out by the Global Health Platform. In the session, titled[...]